Anti-CDC45L antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody (AB230178)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDC45L using ab230178 at a dilution of 1/100 in ICC/IF.

Secondary is an Alexa Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody (AB230178)

Paraffin-embedded human kidney tissue stained for CDC45L with ab230178 at 1/100 dilution in immunohistochemical analysis.

• IP

Supplier Data

Immunoprecipitation - Anti-CDC45L antibody (AB230178)

CDC45L was immunoprecipitated from K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate using ab230178.

Lane 1 : Control IgG IP in K562 whole cell lysate.

Lane 2 : ab230178 in K562 whole cell lysate.

Lane 3 : CDC45L in K562 whole cell lysate (Input).

For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50,000).

All lanes:

Immunoprecipitation - Anti-CDC45L antibody (ab230178)

Predicted band size: 66 kDa

false

• WB

Supplier Data

Western blot - Anti-CDC45L antibody (AB230178)

All lanes:

Western blot - Anti-CDC45L antibody (ab230178) at 1/1000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 66 kDa

Observed band size: 58 kDa,66 kDa

true