Rabbit Recombinant Monoclonal CDC45L antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-CDC45L antibody [EPR5758] (AB108350), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5758-ab108350--western-blot-img105472.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDC45L antibody [EPR5758] (AB108350)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (AB108350), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5758-ab108350--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132299.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (AB108350)")
![OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (AB108350), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5758-ab108350--oi-rd-scanning-img98938.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (AB108350)")
Publications
Filter by
- Cell death & disease 9:10352018Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKui Zhang et. al.PubMed 30305611
- PloS one 8:e613682013Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform antigen retrieval before commencing with IHC staining protocol Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
Alternative names
CDC45L, CDC45L2, UNQ374/PRO710, CDC45, Cell division control protein 45 homolog, PORC-PI-1
Recommended products
Rabbit Recombinant Monoclonal CDC45L antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5758
- Purity
- Tissue culture supernatant
- Dissociation constant
- 3.17 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CDC45L antibody [EPR5758] (ab108350), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5758-ab108350--western-blot-img105472.jpg "Western blot - Anti-CDC45L antibody [EPR5758] (ab108350)")
Western blot - Anti-CDC45L antibody [EPR5758] (ab108350)
All lanes: Western blot - Anti-CDC45L antibody [EPR5758] (ab108350) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
Lane 4: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Predicted band size: 66 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (ab108350), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5758-ab108350--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132299.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (ab108350)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (ab108350)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108350 at 1/50
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (ab108350), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5758-ab108350--oi-rd-scanning-img98938.jpg "OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (ab108350)")
OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (ab108350)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com