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AB108350

Anti-CDC45L antibody [EPR5758]

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(2 Publications)

Rabbit Recombinant Monoclonal CDC45L antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CDC45L, CDC45L2, UNQ374/PRO710, CDC45, Cell division control protein 45 homolog, PORC-PI-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (AB108350)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (AB108350)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108350 at 1/50

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CDC45L antibody [EPR5758] (AB108350)
  • WB

Unknown

Western blot - Anti-CDC45L antibody [EPR5758] (AB108350)

All lanes:

Western blot - Anti-CDC45L antibody [EPR5758] (ab108350) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 66 kDa

false

OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (AB108350)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CDC45L antibody [EPR5758] (AB108350)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5758

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>Perform antigen retrieval before commencing with IHC staining protocol</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
See full target information CDC45
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 9:1035 PubMed30305611

2018

Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Zhang,Gang Fu,Guangzhao Pan,Chongyang Li,Li Shen,Renjian Hu,Shunqin Zhu,Yibiao Chen,Hongjuan Cui

PloS one 8:e61368 PubMed23585894

2013

Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination.

Applications

WB

Species

Unspecified reactive species

Rachel W Anantha,Allen L Alcivar,Jianglin Ma,Hong Cai,Srilatha Simhadri,Jernej Ule,Julian König,Bing Xia
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com