Rabbit Recombinant Monoclonal CDC45L antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (AB240032), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5759-bsa-and-azide-free-ab240032--immunocytochemistry-immunofluorescence-img99968.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (AB240032)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (AB240032), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5759-bsa-and-azide-free-ab240032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72912.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (AB240032)")
![OI-RD Scanning - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (AB240032), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5759-bsa-and-azide-free-ab240032--oi-rd-scanning-img84832.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (AB240032)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes (heat to 98 degrees C, allow to cool for 10-20 minutes) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
Alternative names
CDC45L, CDC45L2, UNQ374/PRO710, CDC45, Cell division control protein 45 homolog, PORC-PI-1
Recommended products
Rabbit Recombinant Monoclonal CDC45L antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5759
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.86 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240032 is the carrier-free version of Anti-CDC45L antibody [EPR5759] ab126762.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5759-bsa-and-azide-free-ab240032--immunocytochemistry-immunofluorescence-img99968.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)")
Immunocytochemistry/ Immunofluorescence - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
ICC/IF image of Anti-CDC45L antibody [EPR5759] ab126762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Anti-CDC45L antibody [EPR5759] ab126762 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC45L antibody [EPR5759] ab126762).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5759-bsa-and-azide-free-ab240032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72912.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
Anti-CDC45L antibody [EPR5759] ab126762, at 1/100 dilution, staining CDC45L in paraffin-embedded Human colon tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC45L antibody [EPR5759] ab126762).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032), expandable thumbnail](https://content.abcam.com/products/images/cdc45l-antibody-epr5759-bsa-and-azide-free-ab240032--oi-rd-scanning-img84832.jpg "OI-RD Scanning - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)")
OI-RD Scanning - Anti-CDC45L antibody [EPR5759] - BSA and Azide free (ab240032)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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Product protocols
- Visit the general protocols
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