AB129114

Anti-CDC5L antibody [EPR7658]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal CDC5L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

KIAA0432, PCDC5RP, CDC5L, Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (AB129114)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (AB129114)

ab129114 at a 1/250 dilution staining CDC5L in paraffin-embedded Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (AB129114)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (AB129114)

ab129114, at a 1/250 dilution, staining CDC5L in HepG2 cells by immunofluorescence.

Unknown

Western blot - Anti-CDC5L antibody [EPR7658] (AB129114)

All lanes:

Western blot - Anti-CDC5L antibody [EPR7658] (ab129114) at 1/1000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 92 kDa

Observed band size: 100 kDa

false

Carrier free

Anti-CDC5L antibody [EPR7658] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7658

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed : 11991638, PubMed : 20176811, PubMed : 28076346, PubMed : 28502770, PubMed : 29301961, PubMed : 29360106, PubMed : 29361316, PubMed : 30705154, PubMed : 30728453). Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR) (PubMed : 20176811). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).

See full target information CDC5L

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 14:3565-3583 PubMed39113868

2024

mA modification of CDC5L promotes lung adenocarcinoma progression through transcriptionally regulating WNT7B expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nanding Yu,Yingxiao Wu,Qiongying Wei,Xiaoping Li,Mengling Li,Weidong Wu

Bioengineered 12:12647-12658 PubMed34903139

2021

Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L).

Applications

Unspecified application

Species

Unspecified reactive species

Kai Wang,Baosong Li,Peng Fan,Xiang Ren,Hong Jiang

BioMed research international 2021:8249293 PubMed34805404

2021

KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Cui-Cui Zhao,Fei-Ran Chen,Guo-Wei Feng,Fei Luo,Tao Jiang

Aging 12:11100-11115 PubMed32526706

2020

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Liu,Dan Guo,Yuou Sha,Chenlu Zhang,Yijing Jiang,Lemin Hong,Jie Zhang,Yuwen Jiang,Ling Lu,Hongming Huang

Autophagy 15:1333-1355 PubMed30767700

2019

Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs.

Applications

Unspecified application

Species

Unspecified reactive species

Åsa Birna Birgisdottir,Stephane Mouilleron,Zambarlal Bhujabal,Martina Wirth,Eva Sjøttem,Gry Evjen,Wenxin Zhang,Rebecca Lee,Nicola O'Reilly,Sharon A Tooze,Trond Lamark,Terje Johansen

Proteomics 14:2350-9 PubMed25065715

2014

Effects of GnRH antagonist on endometrial protein profiles in the window of implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Meng,Yueshuai Guo,Yi Qian,Xuejiang Guo,Li Gao,Jiahao Sha,Yugui Cui,Ri-Cheng Chian,Jiayin Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDC5L antibody [EPR7658]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (AB129114)

Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (AB129114)

Western blot - Anti-CDC5L antibody [EPR7658] (AB129114)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

Product promise