Rabbit Recombinant Monoclonal CDC5L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-CDC5L antibody [EPR7658] (AB129114), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-epr7658-ab129114--western-blot-img124676.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDC5L antibody [EPR7658] (AB129114)")
![Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (AB129114), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-epr7658-ab129114--immunocytochemistry-immunofluorescence-img122192.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (AB129114)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (AB129114), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-epr7658-ab129114--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82104.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (AB129114)")
Publications
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- Bioengineered 12:12647-126582021Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L).Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Wang,Baosong Li,Peng Fan,Xiang Ren,Hong JiangPubMed 34903139
- BioMed research international 2021:82492932021KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Chen,Cui-Cui Zhao,Fei-Ran Chen,Guo-Wei Feng,Fei Luo,Tao JiangPubMed 34805404
- Aging 12:11100-111152020ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyan Liu et. al.PubMed 32526706
- Proteomics 14:2350-92014Effects of GnRH antagonist on endometrial protein profiles in the window of implantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Meng,Yueshuai Guo,Yi Qian,Xuejiang Guo,Li Gao,Jiahao Sha,Yugui Cui,Ri-Cheng Chian,Jiayin LiuPubMed 25065715
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:11991638, PubMed:20176811, PubMed:28076346, PubMed:28502770, PubMed:29301961, PubMed:29360106, PubMed:29361316, PubMed:30705154, PubMed:30728453). Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR) (PubMed:20176811). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Alternative names
KIAA0432, PCDC5RP, CDC5L, Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein
Recommended products
Rabbit Recombinant Monoclonal CDC5L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7658
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CDC5L antibody [EPR7658] (ab129114), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-epr7658-ab129114--western-blot-img124676.jpg "Western blot - Anti-CDC5L antibody [EPR7658] (ab129114)")
Western blot - Anti-CDC5L antibody [EPR7658] (ab129114)
All lanes: Western blot - Anti-CDC5L antibody [EPR7658] (ab129114) at 1/1000 dilution
Lane 1: Fetal brain tissue lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
SecondaryAll lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (ab129114), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-epr7658-ab129114--immunocytochemistry-immunofluorescence-img122192.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (ab129114)")
Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (ab129114)
ab129114, at a 1/250 dilution, staining CDC5L in HepG2 cells by immunofluorescence.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (ab129114), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-epr7658-ab129114--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82104.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (ab129114)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (ab129114)
ab129114, at a 1/250 dilution, staining CDC5L in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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