Rabbit Multiclonal CDC5L antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDC5L.
Images
![Western blot - Anti-CDC5L antibody [RP23040246] (AB308127), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-rp23040246-ab308127--western-blot-img406952.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDC5L antibody [RP23040246] (AB308127)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Recombinant Fragment Protein within Human CDC5L. Database link Q99459
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
Species Mouse | Dilution info 2.5 µg/mL | Notes - |
Target data
Function
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:11991638, PubMed:20176811, PubMed:28076346, PubMed:28502770, PubMed:29301961, PubMed:29360106, PubMed:29361316, PubMed:30705154, PubMed:30728453). Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR) (PubMed:20176811). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Alternative names
KIAA0432, PCDC5RP, CDC5L, Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein
Recommended products
Rabbit Multiclonal CDC5L antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDC5L.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- Recombinant Fragment Protein within Human CDC5L. Database link Q99459
- Clone number
- RP23040246
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-CDC5L antibody [RP23040246] (ab308127), expandable thumbnail](https://content.abcam.com/products/images/cdc5l-antibody-rp23040246-ab308127--western-blot-img406952.jpg "Western blot - Anti-CDC5L antibody [RP23040246] (ab308127)")
Western blot - Anti-CDC5L antibody [RP23040246] (ab308127)
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) Hep G2 (Lane 2), U-2 OS (Lane 3), U-87 MG (Lane 4), T98G (Lane 5), HCT 116 (Lane 6) and NIH/3T3 (Lane 7). The blots were probed with ab308127 at 2.5 µg/mL and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution. A 93 kDa band corresponding to CDC5L was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).
All lanes: Western blot - Anti-CDC5L antibody [RP23040246] (ab308127) at 2.5 µg/mL
Lane 1: HeLa cell lysate at 30 µg
Lane 2: HepG2 cell lysate at 30 µg
Lane 3: U-2 OS cell lysate at 30 µg
Lane 4: U-87 MG cell lysate at 30 µg
Lane 5: T98G cell lysate at 30 µg
Lane 6: HCT 116 cell lysate at 30 µg
Lane 7: NIH/3T3 cell lysate at 30 µg
SecondaryAll lanes: HRP conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution
Developed using the ECL technique.
Observed band size: 92 kDa
Downloads
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