Anti-CDC5L antibody [RP23040246]

• WB

Supplier Data

Western blot - Anti-CDC5L antibody [RP23040246] (AB308127)

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) Hep G2 (Lane 2), U-2 OS (Lane 3), U-87 MG (Lane 4), T98G (Lane 5), HCT 116 (Lane 6) and NIH/3T3 (Lane 7). The blots were probed with ab308127 at 2.5 µg/mL and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution. A 93 kDa band corresponding to CDC5L was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-CDC5L antibody [RP23040246] (ab308127) at 2.5 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

HepG2 cell lysate at 30 µg

Lane 3:

U-2 OS cell lysate at 30 µg

Lane 4:

U-87 MG cell lysate at 30 µg

Lane 5:

T98G cell lysate at 30 µg

Lane 6:

HCT 116 cell lysate at 30 µg

Lane 7:

NIH/3T3 cell lysate at 30 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

Observed band size: 92 kDa

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