AB20150

Anti-Cdc6 antibody [Cdc6 9H8/5]

Name: Anti-Cdc6 antibody [Cdc6 9H8/5] (ab20150) | Abcam
Brand: Abcam Limited
SKU: AB20150
Price: 500 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal CDC6 antibody. Suitable for IP, WB and reacts with Saccharomyces cerevisiae samples. Cited in 7 publications. Immunogen corresponding to Full Length Protein corresponding to Saccharomyces cerevisiae S288C CDC6.

View Alternative Names

YJL194W, J0347, CDC6, Cell division control protein 6

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Cdc6 9H8/5

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

IP, WB

applications

Immunogen

Full Length Protein corresponding to Saccharomyces cerevisiae S288C CDC6. The exact immunogen used to generate this antibody is proprietary information.

P09119

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc6 protein also known simply as Cdc6 plays an important role in the initiation of DNA replication. This protein with a mass of around 60 kDa is widely expressed notably in the nucleus of eukaryotic cells. Cdc6 is responsible for loading the mini-chromosome maintenance (MCM) complex onto DNA an essential step in the formation of the pre-replicative complex at the origins of replication. Acting as an ATPase Cdc6 coordinates with other replication factors during the G1 phase of the cell cycle.

Biological function summary

The Cdc6 protein serves as a regulator of the cell cycle and is part of the origin recognition complex (ORC) which is essential for proper DNA replication initiation. As an important element in the pre-replication complex Cdc6 interacts with Cdt1 to recruit the MCM helicase. This interaction ensures that DNA helicase loading is tightly controlled and regulated during the cell cycle preventing premature replication that could lead to genomic instability.

Pathways

Cdc6 protein plays a vital role in the DNA replication pathway primarily activating at the G1/S transition to initiate replication. Cdc6 associates with other proteins such as ORC and MCM to facilitate the unwinding of DNA and formation of replication forks. Furthermore Cdc6 is integrated into the checkpoint pathways that monitor and control DNA replication and repair processes contributing to maintenance of genomic integrity.

Alterations or dysregulation of the Cdc6 protein are linked to several types of cancers and may contribute to tumorigenesis. Overexpression of Cdc6 has been observed in cancers such as colorectal cancer where it might enhance cellular proliferation through interactions with cyclin proteins. Additionally Cdc6’s role in replication stress has been associated with the development of chromosomal instability syndromes leading to genomic instability and potentially contributing to cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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Target data

Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.

See full target information CDC6

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 38:110531 PubMed35320708

2022

Mobile origin-licensing factors confer resistance to conflicts with RNA polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias J Scherr,Syafiq Abd Wahab,Dirk Remus,Karl E Duderstadt

Nucleic acids research 42:2257-69 PubMed24234446

2013

The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation.

Applications

Species

Unspecified reactive species

Cecile Evrin,Alejandra Fernández-Cid,Alberto Riera,Juergen Zech,Pippa Clarke,M Carmen Herrera,Silvia Tognetti,Rudi Lurz,Christian Speck

Nucleic acids research 41:3162-72 PubMed23376927

2013

In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization.

Applications

Species

Unspecified reactive species

Cecile Evrin,Alejandra Fernández-Cid,Juergen Zech,M Carmen Herrera,Alberto Riera,Pippa Clarke,Shlomo Brill,Rudi Lurz,Christian Speck

PLoS genetics 8:e1003099 PubMed23236290

2012

A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication.

Applications

Species

Unspecified reactive species

Amy E Ikui,Valentina Rossio,Lea Schroeder,Satoshi Yoshida

Cancer research 67:3827-34 PubMed17440097

2007

Tumor necrosis factor receptor 1/c-Jun-NH2-kinase signaling promotes human neoplasia.

Applications

Species

Unspecified reactive species

Jennifer Y Zhang,Amy E Adams,Todd W Ridky,Shiying Tao,Paul A Khavari

The EMBO journal 20:4836-45 PubMed11532947

2001

Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

G Perkins,L S Drury,J F Diffley

Proceedings of the National Academy of Sciences of the United States of America 94:5611-6 PubMed9159120

1997

Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast.

Applications

Unspecified application

Species

Unspecified reactive species

S Donovan,J Harwood,L S Drury,J F Diffley

View all publications

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AB245447

Anti-Cdc6 antibody

Immunoprecipitation - Anti-Cdc6 antibody (AB245447)

Primary Antibodies

AB85830

Anti-ORC1 antibody

Immunoprecipitation - Anti-ORC1 antibody (AB85830)

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