AB188423

Anti-Cdc6 antibody - N-terminal

Name: Anti-Cdc6 antibody - N-terminal (ab188423) | Abcam
Brand: Abcam Limited
SKU: AB188423
Rating: 5 (2 reviews)

(2 Reviews)

(4 Publications)

Rabbit Polyclonal CDC6 antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDC6 aa 1-350.

View Alternative Names

CDC18L, CDC6, Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, p62(cdc6), HsCdc18, HsCDC6

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (AB188423)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (AB188423)

Immunofluorescent analysis of HeLa cells in G1 or early S phase. Cells in mid to late G1phase or hydroxyurea-treated early S phase were fixed with formaldehyde directly or after extraction with Triton X-100 and then immunostained using ab188423 at 1/100 dilution or control non-immune antibody (Alexa;green). The samples were further treated with propidium iodide for DNA staining (PI; red).

Supplier Data

Immunoprecipitation - Anti-Cdc6 antibody - N-terminal (AB188423)

Immunoprecipitation analysis of HeLa whole cell lysate.

Lane 1 : Control immunoprecipitation with non-immune IgG

Lane 2 : Immunoprecipitates wtih ab188423

Lane 3 : Input.

Western blot detection with ab188423 at 1/1000 dilution.

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Immunoprecipitation - Anti-Cdc6 antibody - N-terminal (ab188423)

Predicted band size: 63 kDa

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ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (AB188423)

Immunocytochemistry/ Immunofluorescence analysis of MEFs (mouse embryonic fibroblasts) from embryonic day 14.5 mouse embryos labeling Cdc6 with ab188423 at 1/200 dilution. The cells were fixed with 10 % formalin at room temperature (RT) for 10 min, permeabilized with ice-cold methanol on ice for 10 min, and treated with 2 % BSA/PBS at RT for 1h. Cells were incubated with Anti-Cdc6 antibody - N-terminal (ab188423) at 4°C overnight and then with Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody (1/1000) at RT for 1h. Chromosomal DNA was counterstained with 3.3 μM Hoechst 33342. Note that CDC6 abundantly localizes in nuclei of the cells at G1 and early S phase.

Supplier Data

Western blot - Anti-Cdc6 antibody - N-terminal (AB188423)

All lanes:

Western blot - Anti-Cdc6 antibody - N-terminal (ab188423) at 1/1000 dilution

All lanes:

HeLa cell lysate at 10 µg

Predicted band size: 63 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CDC6 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q99741

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc6 protein also known simply as Cdc6 plays an important role in the initiation of DNA replication. This protein with a mass of around 60 kDa is widely expressed notably in the nucleus of eukaryotic cells. Cdc6 is responsible for loading the mini-chromosome maintenance (MCM) complex onto DNA an essential step in the formation of the pre-replicative complex at the origins of replication. Acting as an ATPase Cdc6 coordinates with other replication factors during the G1 phase of the cell cycle.

Biological function summary

The Cdc6 protein serves as a regulator of the cell cycle and is part of the origin recognition complex (ORC) which is essential for proper DNA replication initiation. As an important element in the pre-replication complex Cdc6 interacts with Cdt1 to recruit the MCM helicase. This interaction ensures that DNA helicase loading is tightly controlled and regulated during the cell cycle preventing premature replication that could lead to genomic instability.

Pathways

Cdc6 protein plays a vital role in the DNA replication pathway primarily activating at the G1/S transition to initiate replication. Cdc6 associates with other proteins such as ORC and MCM to facilitate the unwinding of DNA and formation of replication forks. Furthermore Cdc6 is integrated into the checkpoint pathways that monitor and control DNA replication and repair processes contributing to maintenance of genomic integrity.

Alterations or dysregulation of the Cdc6 protein are linked to several types of cancers and may contribute to tumorigenesis. Overexpression of Cdc6 has been observed in cancers such as colorectal cancer where it might enhance cellular proliferation through interactions with cyclin proteins. Additionally Cdc6’s role in replication stress has been associated with the development of chromosomal instability syndromes leading to genomic instability and potentially contributing to cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

See full target information CDC6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5342 PubMed40533478

2025

Reversible proliferative arrest induced by rapid depletion of RNase MRP.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Liu,Shiyang He,Kawon Pyo,Reuben Franklin,Ibrahim B Maaz,Chen Cai,Kriti Shah,Sihem Cheloufi,William F Marzluff,Jernej Murn

Nature communications 14:5076 PubMed37604829

2023

NFIB facilitates replication licensing by acting as a genome organizer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Zhang,Yue Wang,Yongjie Liu,Cuifang Liu,Yizhou Wang,Lin He,Xiao Cheng,Yani Peng,Lu Xia,Xiaodi Wu,Jiajing Wu,Yu Zhang,Luyang Sun,Ping Chen,Guohong Li,Qiang Tu,Jing Liang,Yongfeng Shang

International journal of molecular sciences 23: PubMed35743017

2022

Inhibition of -Acetyltransferase 10 Suppresses the Progression of Prostate Cancer through Regulation of DNA Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Ningning Ma,Haijing Liu,Yaqian Wu,Mengfei Yao,Bo Zhang

EMBO reports 18:403-419 PubMed28077461

2017

Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation.

Applications

ICC/IF

Species

Human

Shin-Ichiro Hiraga,Tony Ly,Javier Garzón,Zuzana Hořejší,Yoshi-Nobu Ohkubo,Akinori Endo,Chikashi Obuse,Simon J Boulton,Angus I Lamond,Anne D Donaldson

View all publications

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AB245447

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