Rabbit Polyclonal CDC6 antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDC6 aa 1-350.
Images
Publications
Filter by
- Nature communications 14:50762023NFIB facilitates replication licensing by acting as a genome organizer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenting Zhang,Yue Wang,Yongjie Liu,Cuifang Liu,Yizhou Wang,Lin He,Xiao Cheng,Yani Peng,Lu Xia,Xiaodi Wu,Jiajing Wu,Yu Zhang,Luyang Sun,Ping Chen,Guohong Li,Qiang Tu,Jing Liang,Yongfeng ShangPubMed 37604829
- EMBO reports 18:403-4192017Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CDC6 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q99741
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Alternative names
CDC18L, CDC6, Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, p62(cdc6), HsCdc18, HsCDC6
Recommended products
Rabbit Polyclonal CDC6 antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDC6 aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CDC6 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q99741
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Cdc6 protein also known simply as Cdc6 plays an important role in the initiation of DNA replication. This protein with a mass of around 60 kDa is widely expressed notably in the nucleus of eukaryotic cells. Cdc6 is responsible for loading the mini-chromosome maintenance (MCM) complex onto DNA an essential step in the formation of the pre-replicative complex at the origins of replication. Acting as an ATPase Cdc6 coordinates with other replication factors during the G1 phase of the cell cycle.
- Biological function summary
The Cdc6 protein serves as a regulator of the cell cycle and is part of the origin recognition complex (ORC) which is essential for proper DNA replication initiation. As an important element in the pre-replication complex Cdc6 interacts with Cdt1 to recruit the MCM helicase. This interaction ensures that DNA helicase loading is tightly controlled and regulated during the cell cycle preventing premature replication that could lead to genomic instability.
- Pathways
Cdc6 protein plays a vital role in the DNA replication pathway primarily activating at the G1/S transition to initiate replication. Cdc6 associates with other proteins such as ORC and MCM to facilitate the unwinding of DNA and formation of replication forks. Furthermore Cdc6 is integrated into the checkpoint pathways that monitor and control DNA replication and repair processes contributing to maintenance of genomic integrity.
- Associated diseases and disorders
Alterations or dysregulation of the Cdc6 protein are linked to several types of cancers and may contribute to tumorigenesis. Overexpression of Cdc6 has been observed in cancers such as colorectal cancer where it might enhance cellular proliferation through interactions with cyclin proteins. Additionally Cdc6’s role in replication stress has been associated with the development of chromosomal instability syndromes leading to genomic instability and potentially contributing to cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunoprecipitation - Anti-Cdc6 antibody - N-terminal (ab188423)
Immunoprecipitation analysis of HeLa whole cell lysate.
Lane 1: Control immunoprecipitation with non-immune IgG
Lane 2: Immunoprecipitates wtih ab188423
Lane 3: Input.
Western blot detection with ab188423 at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-Cdc6 antibody - N-terminal (ab188423)
Predicted band size: 63 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (ab188423)
Immunocytochemistry/ Immunofluorescence analysis of MEFs (mouse embryonic fibroblasts) from embryonic day 14.5 mouse embryos labeling Cdc6 with ab188423 at 1/200 dilution. The cells were fixed with 10 % formalin at room temperature (RT) for 10 min, permeabilized with ice-cold methanol on ice for 10 min, and treated with 2 % BSA/PBS at RT for 1h. Cells were incubated with Anti-Cdc6 antibody - N-terminal (ab188423) at 4°C overnight and then with Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody (1/1000) at RT for 1h. Chromosomal DNA was counterstained with 3.3 μM Hoechst 33342. Note that CDC6 abundantly localizes in nuclei of the cells at G1 and early S phase.
Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (ab188423)
Immunofluorescent analysis of HeLa cells in G1 or early S phase. Cells in mid to late G1phase or hydroxyurea-treated early S phase were fixed with formaldehyde directly or after extraction with Triton X-100 and then immunostained using ab188423 at 1/100 dilution or control non-immune antibody (Alexa;green). The samples were further treated with propidium iodide for DNA staining (PI; red).
Western blot - Anti-Cdc6 antibody - N-terminal (ab188423)
All lanes: Western blot - Anti-Cdc6 antibody - N-terminal (ab188423) at 1/1000 dilution
All lanes: HeLa cell lysate at 10 µg
Predicted band size: 63 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com