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AB76422

Anti-Cdc6 (phospho S106) antibody [EPR761(2)]

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(3 Publications)

Rabbit Recombinant Monoclonal CDC6 phospho S106 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CDC18L, CDC6, Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, p62(cdc6), HsCdc18, HsCDC6

1 Images
Western blot - Anti-Cdc6 (phospho S106) antibody [EPR761(2)] (AB76422)
  • WB

Unknown

Western blot - Anti-Cdc6 (phospho S106) antibody [EPR761(2)] (AB76422)

All lanes:

Western blot - Anti-Cdc6 (phospho S106) antibody [EPR761(2)] (ab76422) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours whole cell lysate at 15 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours then treated with 10%FBS for 24 hours whole cell lysate at 15 µg

Lane 3:

Raji (Human Burkitt's lymphoma B lymphocyte) starved for 24 hours then treated with 10%FBS for 24 hours whole cell lysate, then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

false

  • Carrier free

    Anti-Cdc6 (phospho S106) antibody [EPR761(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR761(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc6 protein also known simply as Cdc6 plays an important role in the initiation of DNA replication. This protein with a mass of around 60 kDa is widely expressed notably in the nucleus of eukaryotic cells. Cdc6 is responsible for loading the mini-chromosome maintenance (MCM) complex onto DNA an essential step in the formation of the pre-replicative complex at the origins of replication. Acting as an ATPase Cdc6 coordinates with other replication factors during the G1 phase of the cell cycle.
Biological function summary

The Cdc6 protein serves as a regulator of the cell cycle and is part of the origin recognition complex (ORC) which is essential for proper DNA replication initiation. As an important element in the pre-replication complex Cdc6 interacts with Cdt1 to recruit the MCM helicase. This interaction ensures that DNA helicase loading is tightly controlled and regulated during the cell cycle preventing premature replication that could lead to genomic instability.

Pathways

Cdc6 protein plays a vital role in the DNA replication pathway primarily activating at the G1/S transition to initiate replication. Cdc6 associates with other proteins such as ORC and MCM to facilitate the unwinding of DNA and formation of replication forks. Furthermore Cdc6 is integrated into the checkpoint pathways that monitor and control DNA replication and repair processes contributing to maintenance of genomic integrity.

Alterations or dysregulation of the Cdc6 protein are linked to several types of cancers and may contribute to tumorigenesis. Overexpression of Cdc6 has been observed in cancers such as colorectal cancer where it might enhance cellular proliferation through interactions with cyclin proteins. Additionally Cdc6’s role in replication stress has been associated with the development of chromosomal instability syndromes leading to genomic instability and potentially contributing to cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
See full target information CDC6 phospho S106
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38866486

2024

Sex Differences in Dopamine Release in Nucleus Accumbens and Dorsal Striatum Determined by Chronic Fast-Scan Cyclic Voltammetry: Effects of Social Housing and Repeated Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ivette L Gonzalez,Christopher A Turner,Paras R Patel,Noah B Leonardo,Brandon D Luma,Julianna M Richie,Dawen Cai,Cynthia A Chestek,Jill B Becker

Science advances 5:eaav3660 PubMed31131319

2019

Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichi Iwai,Tadahiro Nambu,Ryo Dairiki,Momoko Ohori,Jie Yu,Kristine Burke,Masamitsu Gotou,Yukiko Yamamoto,Shunsuke Ebara,Sachio Shibata,Ryosuke Hibino,Satoru Nishizawa,Tohru Miyazaki,Misaki Homma,Yuya Oguro,Takashi Imada,Nobuo Cho,Noriko Uchiyama,Akifumi Kogame,Toshiyuki Takeuchi,Osamu Kurasawa,Kazunori Yamanaka,Huifeng Niu,Akihiro Ohashi

Frontiers in synaptic neuroscience 6:11 PubMed24822045

2014

D-amino acid oxidase is expressed in the ventral tegmental area and modulates cortical dopamine.

Applications

IF

Species

Unspecified reactive species

Jill F Betts,Judith V Schweimer,Katherine E Burnham,Philip W J Burnet,Trevor Sharp,Paul J Harrison
View all publications
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Product promise

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