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AB75809

Anti-Cdc6 (phospho S54) antibody [EPR759Y]

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(5 Publications)

Rabbit Recombinant Monoclonal CDC6 phospho S54 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 5 publications.

View Alternative Names

CDC18L, CDC6, Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, p62(cdc6), HsCdc18, HsCDC6

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)

Cell line 293T (Human embryonic kidney epithelial cell). Target AbID ab75809 anti- Cdc6 (phospho S54)

ab150077 AlexaFluor®488 Goat anti-Rabbit secondary. Counterstain AbID ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Fixative 4% Paraformaldehyde. Permeabilisation 0.1% tritonX-100. Nuclear counter stain DAPI. Datasheet Comments Confocal image showing the expression was increased after treatment with Hydroxyurea (3mM 20hour), the signal was decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h. Target 1oAb dilution 1 : 100 2.4 μg/ml, Target 2ndry Ab dilution 1 : 1000 2 μg/ml, Counterstain Ab dilution 1 : 200 2.5 μg/ml

Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)
  • WB

Lab

Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)

Blocking buffer and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/5000 dilution

Lane 1:

Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h) Whole cell lysates at 15 µg

Lane 3:

Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h) Whole cell lysates, Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

false

Exposure time: 30s

Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)
  • WB

Unknown

Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)

All lanes:

Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/2000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Raji + FBS cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

Dot Blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)
  • Dot

Supplier Data

Dot Blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (AB75809)

Primary antibody dilution 1 : 1000 dilution. Secondary antibody used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1 : 100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM /TBST. Lane 1 : Cdc6 (pS54) phospho peptide, Lane 2 : Cdc6 non-phospho peptide. Exposure time 3 minutes

  • Carrier free

    Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR759Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc6 protein also known simply as Cdc6 plays an important role in the initiation of DNA replication. This protein with a mass of around 60 kDa is widely expressed notably in the nucleus of eukaryotic cells. Cdc6 is responsible for loading the mini-chromosome maintenance (MCM) complex onto DNA an essential step in the formation of the pre-replicative complex at the origins of replication. Acting as an ATPase Cdc6 coordinates with other replication factors during the G1 phase of the cell cycle.
Biological function summary

The Cdc6 protein serves as a regulator of the cell cycle and is part of the origin recognition complex (ORC) which is essential for proper DNA replication initiation. As an important element in the pre-replication complex Cdc6 interacts with Cdt1 to recruit the MCM helicase. This interaction ensures that DNA helicase loading is tightly controlled and regulated during the cell cycle preventing premature replication that could lead to genomic instability.

Pathways

Cdc6 protein plays a vital role in the DNA replication pathway primarily activating at the G1/S transition to initiate replication. Cdc6 associates with other proteins such as ORC and MCM to facilitate the unwinding of DNA and formation of replication forks. Furthermore Cdc6 is integrated into the checkpoint pathways that monitor and control DNA replication and repair processes contributing to maintenance of genomic integrity.

Alterations or dysregulation of the Cdc6 protein are linked to several types of cancers and may contribute to tumorigenesis. Overexpression of Cdc6 has been observed in cancers such as colorectal cancer where it might enhance cellular proliferation through interactions with cyclin proteins. Additionally Cdc6’s role in replication stress has been associated with the development of chromosomal instability syndromes leading to genomic instability and potentially contributing to cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
See full target information CDC6 pS54
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 14:446-467 PubMed38047585

2023

INX-315, a Selective CDK2 Inhibitor, Induces Cell Cycle Arrest and Senescence in Solid Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Dietrich,Alec Trub,Antonio Ahn,Michael Taylor,Krutika Ambani,Keefe T Chan,Kun-Hui Lu,Christabella A Mahendra,Catherine Blyth,Rhiannon Coulson,Susanne Ramm,April C Watt,Sunil Kumar Matsa,John Bisi,Jay Strum,Patrick Roberts,Shom Goel

Oncogene 37:3763-3777 PubMed29636547

2018

BRD4 facilitates replication stress-induced DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Zhang,Austin M Dulak,Maureen M Hattersley,Brandon S Willis,Jenni Nikkilä,Anderson Wang,Alan Lau,Corinne Reimer,Michael Zinda,Stephen E Fawell,Gordon B Mills,Huawei Chen

Aging cell 16:575-584 PubMed28345297

2017

Residual Cdk1/2 activity after DNA damage promotes senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Müllers,Helena Silva Cascales,Kamila Burdova,Libor Macurek,Arne Lindqvist

Cell reports 18:1970-1981 PubMed28228262

2017

Targeting DNA Damage Response in Prostate Cancer by Inhibiting Androgen Receptor-CDC6-ATR-Chk1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Styliani Karanika,Theodoros Karantanos,Likun Li,Jianxiang Wang,Sanghee Park,Guang Yang,Xuemei Zuo,Jian H Song,Sankar N Maity,Ganiraju C Manyam,Bradley Broom,Ana M Aparicio,Gary E Gallick,Patricia Troncoso,Paul G Corn,Nora Navone,Wei Zhang,Shuhua Li,Timothy C Thompson

Carcinogenesis 37:799-809 PubMed27207654

2016

Role of Cdc6 in re-replication in cells expressing human papillomavirus E7 oncogene.

Applications

Unspecified application

Species

Unspecified reactive species

Xueli Fan,Yunying Zhou,Jason J Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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