Anti-CDC7 antibody [EPR20337]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (AB229187)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling CDC7 with ab229187 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of human testis (PMID : 9250678) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (AB229187)

Immunohistochemical analysis of paraffin-embedded human normal breast tissue (panel A) and human breast cancer tissue (panel B) labeling CDC7 with ab229187 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Weak nuclear Cdc7 expression (arrow) found in human breast epithelial cells while strong nuclear Cdc7 expression found in human breast cancer cells (PMID : 19896697) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

• IP

Supplier Data

Immunoprecipitation - Anti-CDC7 antibody [EPR20337] (AB229187)

CDC7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab229187 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229187 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).
Lane 2 : ab229187 IP in HeLa whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229187 in HeLa whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CDC7 antibody [EPR20337] (ab229187)

Predicted band size: 64 kDa

Observed band size: 64 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (AB229187)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling CDC7 with ab229187 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of rat testis is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC7 antibody [EPR20337] (AB229187)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CDC7 with ab229187 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in germ cells of mouse testis (PMID : 9722556) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

• WB

Supplier Data

Western blot - Anti-CDC7 antibody [EPR20337] (AB229187)

Blocking/Dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument (PMID : 19318489).

All lanes:

Western blot - Anti-CDC7 antibody [EPR20337] (ab229187) at 1/1000 dilution

Lane 1:

WI-38 (human fetal lung fibroblast) serum deprived (0.1% FBS) for 72 hours, whole cell lysate at 10 µg

Lane 2:

WI-38 serum deprived (0.1% FBS) for 72 hours, then added 10% FBS for 18 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-CDC7 antibody [EPR20337] (AB229187)

Blocking/Dilution buffer : 5%NFDM/TBST.

Exposure times :

Lanes 1-2 : 3 minutes

Lane 3 : 32 seconds

Lane 3 was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-CDC7 antibody [EPR20337] (ab229187) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

true