AB108332

Anti-CDC7 antibody [EPR4730]

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal CDC7 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CDC7L1, CDC7, Cell division cycle 7-related protein kinase, CDC7-related kinase, HsCdc7, huCdc7

3 Images

Flow Cytometry (Intracellular) - Anti-CDC7 antibody [EPR4730] (AB108332)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CDC7 antibody [EPR4730] (AB108332)

ab108332, at 1/100 dilution, staining CDC7 in permeabilized Jurkat cells by intracellular flow cytometry (red). Rabbit IgG (green).

Unknown

Western blot - Anti-CDC7 antibody [EPR4730] (AB108332)

All lanes:

Western blot - Anti-CDC7 antibody [EPR4730] (ab108332) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

PC3 cell lysate at 10 µg

Predicted band size: 64 kDa

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CiteAb

Western blot - Anti-CDC7 antibody [EPR4730] (AB108332)

CDC7 western blot using anti-CDC7 antibody [EPR4730] ab108332. Publication image and figure legend from Zhen, H., Zhang, Y., et al., 2014, Evid Based Complement Alternat Med, PubMed 24511319.

ab108332 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108332 please see the product overview.

Effect of the Toona sinensis and Moschus decoction on the expression of p27, CDK7, CDC7, and Cyclin E1 in HeLa cells by western blot. GAPDH was used as the loading control.

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Carrier free

Anti-CDC7 antibody [EPR4730] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4730

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kinase involved in initiation of DNA replication. Phosphorylates critical substrates that regulate the G1/S phase transition and initiation of DNA replication, such as MCM proteins and CLASPIN.

See full target information CDC7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 19: PubMed30126925

2018

Functional interplay between c-Myc and Max in B lymphocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Mercedes Pérez-Olivares,Alfonsina Trento,Sara Rodriguez-Acebes,Daniel González-Acosta,David Fernández-Antorán,Sara Román-García,Dolores Martinez,Tania López-Briones,Carlos Torroja,Yolanda R Carrasco,Juan Méndez,Ignacio Moreno de Alborán

Translational oncology 11:300-306 PubMed29413763

2018

CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Li,Wanfu Xie,Ning Wang,Chuankun Li,Maode Wang

Evidence-based complementary and alternative medicine : eCAM 2014:121276 PubMed24511319

2014

Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells.

Applications

Species

Human

Hong Zhen,Yifei Zhang,Zhijia Fang,Zhiwei Huang,Chongge You,Ping Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDC7 antibody [EPR4730]

Flow Cytometry (Intracellular) - Anti-CDC7 antibody [EPR4730] (AB108332)

Western blot - Anti-CDC7 antibody [EPR4730] (AB108332)

Western blot - Anti-CDC7 antibody [EPR4730] (AB108332)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Product promise