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Rabbit Recombinant Monoclonal CDCA5 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328), expandable thumbnail
  • Western blot - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (AB240328), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested

Expected
Expected

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.

Alternative names

Sororin, Cell division cycle-associated protein 5, p35, CDCA5

Recommended products

Rabbit Recombinant Monoclonal CDCA5 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16331
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240328 is the carrier-free version of Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    Negative controls: anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237).

  • Flow Cytometry (Intracellular) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328)

    Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5 with Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328)

    Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237 at 1/100 (5 μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237 at 1/100 (5 μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240328).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328), expandable thumbnail

    Western blot - Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237).

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    Blocking and diluting buffer and concntration: 5% NFDM/TBST

    Imaging carried out using iBright CL 1000 Imaging System

    Exposure time: Lane 1: 180 seconds, Lane 2: 48 seconds.

    All lanes: Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (Anti-CDCA5 antibody [EPR16331] - C-terminal ab192237) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 28 kDa

    Observed band size: 27 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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