Anti-CDCA5 antibody [EPR16331] - C-terminal

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (AB192237)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (AB192237)

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls : anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (AB192237)

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CDCA5 antibody [EPR16331] - C-terminal (AB192237)

Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

• WB

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Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (AB192237)

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Blocking and diluting buffer and concntration : 5% NFDM/TBST Imaging carried out using iBright CL 1000 Imaging System Exposure time : Lane 1 : 180 seconds, Lane 2 : 48 seconds.

All lanes:

Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 27 kDa

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