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AB246958

Anti-CDCA7L/HR1 antibody

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(2 Publications )

Rabbit Polyclonal CDCA7L/HR1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDCA7L aa 50 to C-terminus.
4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CDCA7L/HR1 antibody (AB246958)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDCA7L/HR1 antibody (AB246958)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CDCA7L/HR1 (green) using ab246958 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA7L/HR1 antibody (AB246958)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA7L/HR1 antibody (AB246958)

Paraffin-embedded human appendix tissue stained for CDCA7L/HR1 using ab246958 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-CDCA7L/HR1 antibody (AB246958)
  • WB

Unknown

Western blot - Anti-CDCA7L/HR1 antibody (AB246958)

All lanes:

Western blot - Anti-CDCA7L/HR1 antibody (ab246958) at 0.4 µg/mL

All lanes:

NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate

Predicted band size: 52 kDa

false

Western blot - Anti-CDCA7L/HR1 antibody (AB246958)
  • WB

Unknown

Western blot - Anti-CDCA7L/HR1 antibody (AB246958)

All lanes:

Western blot - Anti-CDCA7L/HR1 antibody (ab246958) at 0.4 µg/mL

All lanes:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CDCA7L aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96GN5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. Involved in apoptotic signaling pathways; May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.
See full target information CDCA7L

Alternative Names

HR1, JPO2, R1, CDCA7L, Cell division cycle-associated 7-like protein, Protein JPO2, Transcription factor RAM2
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Autophagy 20:114-130 PubMed37615625

2023

Tumor-derived lactate promotes resistance to bevacizumab treatment by facilitating autophagy enhancer protein RUBCNL expression through histone H3 lysine 18 lactylation (H3K18la) in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weihao Li,Chi Zhou,Long Yu,Zhenlin Hou,Huashan Liu,Lingheng Kong,Yanbo Xu,Jiahua He,Jin Lan,Qingjian Ou,Yujing Fang,Zhenhai Lu,Xiaojun Wu,Zhizhong Pan,Jianhong Peng,Junzhong Lin

Cell cycle (Georgetown, Tex.) 19:870-883 PubMed32178558

2020

LncRNA LEF1-AS1 silencing diminishes EZH2 expression to delay hepatocellular carcinoma development by impairing CEBPB-interaction with CDCA7.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Gao,Chao Dai,Xin Yu,Xiang-Bao Yin,Fan Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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