AB32384

Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161]

Name: Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) | Abcam
Brand: Abcam Limited
SKU: AB32384
Price: 230 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(35 Publications)

Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) is a rabbit monoclonal antibody detecting CDK1 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
- Trusted since 2006

View Alternative Names

CDC2, CDC28A, CDKN1, P34CDC2, CDK1, Cyclin-dependent kinase 1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDKN2, CDK2, Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDKN3, CDK3, Cyclin-dependent kinase 3, Cell division protein kinase 3

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab32384 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Immunohistochemical staining of paraffin embedded human B cell lymphoma with purified ab32384 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunocytochemistry/ Immunofluorescence - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Immunofluorescence staining of HeLa cells with purified ab32384 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32384 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

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Immunoprecipitation - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

ab32384 (purified) at 1/50 immunoprecipitating CDK1 in 10 μg HEK293 (Lanes 1 and 2, observed at 34 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384)

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Lab

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Blocking/Diluting buffer 5% NFDM/TBST

34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.

All lanes:

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/2000 dilution

Lane 1:

Empty vector transfected 293T (human embryonic kidney) whole cell lysates at 15 µg

Lane 2:

CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates at 15 µg

Lane 3:

CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates at 15 µg

Lane 4:

CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates at 15 µg

Lane 5:

CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa,62 kDa

false

Exposure time: 15s

Unknown

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

All lanes:

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SKBR3 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

false

Lab

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Blocking/Diluting buffer

All lanes:

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/2000 dilution

Lane 1:

Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 15 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 3mM hydroxyurea for 20 hours whole cell lysates at 15 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 3mM hydroxyurea for 20 hours whole cell lysates, then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa

false

Exposure time: 30s

Lab

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/5000 dilution

All lanes:

Raw264.7 cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Observed band size: 34 kDa

false

Lab

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/10000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Observed band size: 34 kDa

false

Carrier free

Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] - BSA and Azide free
HRP

HRP Anti-CDK1 + CDK2 + CDK3 (phospho T14) antibody [E161]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E161

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CDK1?
Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) [E161] (ab32384) specifically detects a band for CDK1 (UniProt: P06493) at a molecular weight of 34kDa.

Trusted by the scientific community
Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) [E161] (ab32384) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [E161] also available for your convenience: ab32384, HRP - ab193834, Carrier free - ab219586, Alexa Fluor® 488 - ab314672, Alexa Fluor® 647 - ab314673

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase cyclins (PubMed : 16407259, PubMed : 16933150, PubMed : 17459720, PubMed : 18356527, PubMed : 19509060, PubMed : 19917720, PubMed : 20171170, PubMed : 20935635, PubMed : 20937773, PubMed : 21063390, PubMed : 2188730, PubMed : 23355470, PubMed : 2344612, PubMed : 23601106, PubMed : 23602554, PubMed : 25556658, PubMed : 26829474, PubMed : 27814491, PubMed : 30139873, PubMed : 30704899, PubMed : 40440427). Phosphorylates PARVA/actopaxin, APC, AMPH, APC, ASB7, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, UHRF1, KAT5, LMNA, LMNB, LBR, MKI67, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MLST8, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, TPPP, UL40/R2, RAB4A, RAP1GAP, RBBP8/CtIP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SAMHD1, SIRT2, CGAS and RUNX2 (PubMed : 16407259, PubMed : 16933150, PubMed : 17459720, PubMed : 18356527, PubMed : 19202191, PubMed : 19509060, PubMed : 19917720, PubMed : 20171170, PubMed : 20935635, PubMed : 20937773, PubMed : 21063390, PubMed : 2188730, PubMed : 22411829, PubMed : 23355470, PubMed : 2344612, PubMed : 23601106, PubMed : 23602554, PubMed : 25012651, PubMed : 25556658, PubMed : 26829474, PubMed : 27814491, PubMed : 30704899, PubMed : 32351706, PubMed : 34741373, PubMed : 40440427). CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs (PubMed : 18480403, PubMed : 20360007). Essential for early stages of embryonic development (PubMed : 18480403, PubMed : 20360007). During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation (PubMed : 18480403, PubMed : 20360007, PubMed : 2188730, PubMed : 2344612, PubMed : 30139873). Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis (PubMed : 18480403, PubMed : 20360007). Phosphorylates KRT5 during prometaphase and metaphase (By similarity). Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair (PubMed : 20360007). Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression (PubMed : 20395957). Catalyzes lamin (LMNA, LMNB1 and LMNB2) phosphorylation at the onset of mitosis, promoting nuclear envelope breakdown (PubMed : 2188730, PubMed : 2344612, PubMed : 37788673). In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons (PubMed : 18356527). The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis (PubMed : 16371510). NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation (PubMed : 19509060). In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis (PubMed : 20171170). The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis (PubMed : 19917720). In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis (PubMed : 20937773). This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes (PubMed : 20937773). EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing (PubMed : 20935635). CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity). CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed : 26549230). Regulates the amplitude of the cyclic expression of the core clock gene BMAL1 by phosphorylating its transcriptional repressor NR1D1, and this phosphorylation is necessary for SCF(FBXW7)-mediated ubiquitination and proteasomal degradation of NR1D1 (PubMed : 27238018). Phosphorylates EML3 at 'Thr-881' which is essential for its interaction with HAUS augmin-like complex and TUBG1 (PubMed : 30723163). Phosphorylates CGAS during mitosis, leading to its inhibition, thereby preventing CGAS activation by self DNA during mitosis (PubMed : 32351706). Phosphorylates SKA3 on multiple sites during mitosis which promotes SKA3 binding to the NDC80 complex and anchoring of the SKA complex to kinetochores, to enable stable attachment of mitotic spindle microtubules to kinetochores (PubMed : 28479321, PubMed : 31804178, PubMed : 32491969).. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry.

See full target information CDK1

Additional targets

CDK2,

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

International journal of cell biology 2024:6798897 PubMed38716144

2024

Mitotic Kinases Aurora-A, Plk1, and Cdk1 Interact with Elk-1 Transcription Factor through the N-Terminal Domain.

Applications

Unspecified application

Species

Unspecified reactive species

Oya Arı Uyar,Yigit Koray Babal,Bayram Yılmaz,Isil Aksan Kurnaz

Frontiers in cell and developmental biology 10:760466 PubMed35186916

2022

The Circular RNA CircCOL1A1 Functions as a miR-149-5p Sponge to Regulate the Formation of Superior-Quality Brush Hair the CMTM3/AR Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Xi Wu,Xiaomei Sun,Liuming Zhang,Qiang Wang,Jingwen Qu,Yanhu Wang,Yongjun Li

Proteomics 22:e2100141 PubMed34932872

2022

Comprehensive kinomic study via a chemical proteomic approach reveals kinome reprogramming in hepatocellular carcinoma tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Wang,Xinzheng Wang,Xin Yang,Feng Liu,Jin Li,Weihua Li,Zhaofang Bai,Hongbo Wang,Jie Mao,Tingting Li,Kun He,Hongxia Wang

Cancers 13: PubMed34359707

2021

ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status.

Applications

Unspecified application

Species

Unspecified reactive species

Gisel Barés,Aida Beà,Luís Hernández,Raul Navaridas,Isidre Felip,Cristina Megino,Natividad Blasco,Ferran Nadeu,Elías Campo,Marta Llovera,Xavier Dolcet,Daniel Sanchis

Cellular and molecular gastroenterology and hepatology 12:633-651 PubMed33848642

2021

Mettl14-Mediated mA Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyue Cao,Yuke Shu,Yuwei Chen,Qing Xu,Gang Guo,Zhenru Wu,Mingyang Shao,Yongjie Zhou,Menglin Chen,Yuping Gong,Chuan Li,Yujun Shi,Hong Bu

Molecular cancer research : MCR 19:1156-1167 PubMed33753551

2021

AP-2α Regulates S-Phase and Is a Marker for Sensitivity to PI3K Inhibitor Buparlisib in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anna C Beck,Edward Cho,Jeffrey R White,Lily Paemka,Tiandao Li,Vivian W Gu,Dakota T Thompson,Kelsey E Koch,Christopher Franke,Matthew Gosse,Vincent T Wu,Shannon R Landers,Anthony J Pamatmat,Mikhail V Kulak,Ronald J Weigel

Redox biology 37:101736 PubMed33032073

2020

Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Natividad Blasco,Aida Beà,Gisel Barés,Cristina Girón,Raúl Navaridas,Andrea Irazoki,Guillermo López-Lluch,Antonio Zorzano,Xavier Dolcet,Marta Llovera,Daniel Sanchis

Cellular oncology (Dordrecht) : PubMed32761561

2020

[10]-Gingerol improves doxorubicin anticancer activity and decreases its side effects in triple negative breast cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Carolina Baptista Moreno Martin,Rebeka Tomasin,Liany Luna-Dulcey,Angélica Elen Graminha,Marina Araújo Naves,Ramon Handerson Gomes Teles,Vinicius Duval da Silva,James Almada da Silva,Paulo Cezar Vieira,Borhane Annabi,Márcia Regina Cominetti

Cell cycle (Georgetown, Tex.) 19:2007-2017 PubMed32646340

2020

Circular RNA circDLGAP4 is involved in lung cancer development through modulating microRNA-143/CDK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Peiyan Hua,Bin Zhao,Jindong Li,Yan Zhang

CNS neuroscience & therapeutics : PubMed32602622

2020

Allopregnanolone restores the tyrosine hydroxylase-positive neurons and motor performance in a 6-OHDA-injected mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Chi Chen,Tong-Tong Wang,Wei Bian,Xin Ye,Meng-Yi Li,Juan-Juan Du,Peng Zhou,Huai-Rui Cui,Yu-Qiang Ding,Yan-Hua Ren,Shuang-Shuang Qi,Yuan-Yuan Yuan,Min Liao,Chen-You Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Immunocytochemistry/ Immunofluorescence - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Immunoprecipitation - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Western blot - Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (AB32384)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (35)

Product promise