Rabbit Recombinant Monoclonal CDK1 phospho Y15 antibody. Suitable for IHC-P, ELISA, Dot, WB and reacts with Human, Rat, Mouse, Recombinant fragment samples. Cited in 14 publications.
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Publications
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- Oncogenesis 9:882020Overexpression of Cyclin E1 or Cdc25A leads to replication stress, mitotic aberrancies, and increased sensitivity to replication checkpoint inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYannick P Kok et. al.PubMed 33028815
- Cell reports 32:1078582020Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen Gray,Emerson R Santiago,Joshua S Chappie,Paula E CohenPubMed 32640224
- Clinical cancer research : an official journal of the American Association for Cancer Research 26:1667-16772019Identification of Therapeutic Vulnerabilities in Small-cell Neuroendocrine Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra N Corella,Ma Victoria Andrea Cabiliza Ordonio,Ilsa Coleman,Jared M Lucas,Arja Kaipainen,Holly M Nguyen,Daniel Sondheim,Lisha G Brown,Lawrence D True,John K Lee,David MacPherson,Paul Nghiem,Roman Gulati,Colm Morrissey,Eva Corey,Peter S NelsonPubMed 31806643
- Cell reports 28:2345-2357.e52019Modeling of Cisplatin-Induced Signaling Dynamics in Triple-Negative Breast Cancer Cells Reveals Mediators of Sensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Margriet Heijink,Marieke Everts,Megan E Honeywell,Ryan Richards,Yannick P Kok,Elisabeth G E de Vries,Michael J Lee,Marcel A T M van VugtPubMed 31461651
- Cells 8:2019C/EBPβ Is a Transcriptional Regulator of Wee1 at the G₂/M Phase of the Cell Cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Hae Lee et. al.PubMed 30754676
- Molecular medicine reports 17:5095-51012018Paeoniflorin induces G2/M cell cycle arrest and caspase-dependent apoptosis through the upregulation of Bcl-2 X-associated protein and downregulation of B-cell lymphoma 2 in human osteosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Bin Jin et. al.PubMed 29363721
- Cellular physiology and biochemistry : internation 44:423-4352017Roscovitine, a CDK5 Inhibitor, Alleviates Sevoflurane-Induced Cognitive Dysfunction via Regulation Tau/GSK3β and ERK/PPARγ/CREB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhui Liu et. al.PubMed 29141245
- PloS one 11:e01626332016Cell Division Cycle 6 Promotes Mitotic Slippage and Contributes to Drug Resistance in Paclitaxel-Treated Cancer Cells.
- PloS one 11:e01588442016Identification of Phosphorylated Cyclin-Dependent Kinase 1 Associated with Colorectal Cancer Survival Using Label-Free Quantitative Analyses.
- Autophagy 12:1472-862016Atg5-mediated autophagy deficiency in proximal tubules promotes cell cycle G2/M arrest and renal fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiyan Li et. al.PubMed 27304991
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ELISA | Dot | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Expected | Expected | Expected | Tested |
| Recombinant fragment | Not recommended | Tested | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/75 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 - 1/75 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/50 - 1/75 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Target data
Function
Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase cyclins (PubMed:16407259, PubMed:16933150, PubMed:17459720, PubMed:18356527, PubMed:19509060, PubMed:19917720, PubMed:20171170, PubMed:20935635, PubMed:20937773, PubMed:21063390, PubMed:2188730, PubMed:23355470, PubMed:2344612, PubMed:23601106, PubMed:23602554, PubMed:25556658, PubMed:26829474, PubMed:27814491, PubMed:30139873, PubMed:30704899). Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, KAT5, LMNA, LMNB, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MLST8, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, TPPP, UL40/R2, RAB4A, RAP1GAP, RBBP8/CtIP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SAMHD1, SIRT2, CGAS and RUNX2 (PubMed:16407259, PubMed:16933150, PubMed:17459720, PubMed:18356527, PubMed:19202191, PubMed:19509060, PubMed:19917720, PubMed:20171170, PubMed:20935635, PubMed:20937773, PubMed:21063390, PubMed:2188730, PubMed:23355470, PubMed:2344612, PubMed:23601106, PubMed:23602554, PubMed:25556658, PubMed:26829474, PubMed:27814491, PubMed:30704899, PubMed:32351706, PubMed:34741373). CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs (PubMed:18480403, PubMed:20360007). Essential for early stages of embryonic development (PubMed:18480403, PubMed:20360007). During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation (PubMed:18480403, PubMed:20360007, PubMed:2188730, PubMed:2344612, PubMed:30139873). Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis (PubMed:18480403, PubMed:20360007). Phosphorylates KRT5 during prometaphase and metaphase (By similarity). Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair (PubMed:20360007). Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression (PubMed:20395957). Catalyzes lamin (LMNA, LMNB1 and LMNB2) phosphorylation at the onset of mitosis, promoting nuclear envelope breakdown (PubMed:2188730, PubMed:2344612, PubMed:37788673). In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons (PubMed:18356527). The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis (PubMed:16371510). NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation (PubMed:19509060). In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis (PubMed:20171170). The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis (PubMed:19917720). In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis (PubMed:20937773). This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes (PubMed:20937773). EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing (PubMed:20935635). CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity). CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed:26549230). Regulates the amplitude of the cyclic expression of the core clock gene BMAL1 by phosphorylating its transcriptional repressor NR1D1, and this phosphorylation is necessary for SCF(FBXW7)-mediated ubiquitination and proteasomal degradation of NR1D1 (PubMed:27238018). Phosphorylates EML3 at 'Thr-881' which is essential for its interaction with HAUS augmin-like complex and TUBG1 (PubMed:30723163). Phosphorylates CGAS during mitosis, leading to its inhibition, thereby preventing CGAS activation by self DNA during mitosis (PubMed:32351706). Phosphorylates SKA3 on multiple sites during mitosis which promotes SKA3 binding to the NDC80 complex and anchoring of the SKA complex to kinetochores, to enable stable attachment of mitotic spindle microtubules to kinetochores (PubMed:28479321, PubMed:31804178, PubMed:32491969). (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry.
Alternative names
CDC2, CDC28A, CDKN1, P34CDC2, CDK1, Cyclin-dependent kinase 1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase
Recommended products
Rabbit Recombinant Monoclonal CDK1 phospho Y15 antibody. Suitable for IHC-P, ELISA, Dot, WB and reacts with Human, Rat, Mouse, Recombinant fragment samples. Cited in 14 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7875
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody will cross-react with phosphorylated CDK1 (pY15), CDK2 (pY15), CDK3 (pY15) and CDK5 (pY15) but not with non-phosphorylated CDK1, CDK2, CDK3 and CDK5. Please see our ELISA results on the images section.
- Dissociation constant
- 4.1 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463) at 1/1000 dilution
All lanes: HeLa cell lysate treated with UV at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Observed band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Immunohistochemical staining of paraffin embedded human colon with purified ab133463 at a working dilution of 1 in 75. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
ELISA - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Direct ELISA antibody dose-response curve using purified ab133463 at 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
ELISA - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Direct ELISA antibody dose-response curve using purified ab133463 at 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463) at 1/2000 dilution
All lanes: C6 cell lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Observed band size: 34 kDa
Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463) at 1/2000 dilution
All lanes: NIH/3T3 cell lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Observed band size: 34 kDa
Dot Blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Dot blot analysis of CDK1+CDK2+CDK3+CDK5 (pY15) phospho peptide (lane 1) and CDK1+CDK2+CDK3+CDK5 non-phospho peptide (lane 2) labelling CDK1+CDK2+CDK3+CDK5 (phospho Y15) with unpurified ab133463 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Saso2 cells were incubated at 37°C for 24 hours with vehicle control (0 μM) and different concentrations of Amifostine (Amifostine, cytoprotective agent ab141060). Decreased expression of CDK1 (phospho Y15) (unpurified ab133463) in Saso2 cells correlates with an increase in Amifostine concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab133463 at 1 μg/ml and Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] ab32384 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 and visualised using ECL development solution.
All lanes: Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 34 kDa
Exposure time: 4min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labelling CDK1+CDK2+CDK3+CDK5 with unpurified ab133463 at 1/50 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463) at 1/1500 dilution
All lanes: NIH/3T3 cell lysate at 1/1000 dilution
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Observed band size: 34 kDa
Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463) at 1/1500 dilution
All lanes: C6 cell lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Observed band size: 34 kDa
OI-RD Scanning - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
All lanes: Western blot - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HeLa cell lysate treated with UV at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Observed band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho Y15) + CDK2 (phospho Y15) + CDK3 (phospho Y15) + CDK5 (phospho Y15) antibody [EPR (ab133463)
Immunohistochemical staining of paraffin embedded human colon with unpurified ab133463 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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