AB19393
Anti-CDK11/CDC2L2/CDK11A antibody
4
(5 Reviews)
|
(10 Publications)
Rabbit Polyclonal CDK11/CDC2L2/CDK11A antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications.
View Alternative Names
CDC2L2, CDC2L3, PITSLREB, CDK11A, Cyclin-dependent kinase 11A, Cell division cycle 2-like protein kinase 2, Cell division protein kinase 11A, Galactosyltransferase-associated protein kinase p58/GTA, PITSLRE serine/threonine-protein kinase CDC2L2
3 Images
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK11/CDC2L2/CDK11A antibody (AB19393)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human lung carcinoma tissue labelling CDK11/CDC2L2/CDK11A with ab19393 at 1/1000 (1μg/ml). Detection : DAB.
- IP
Supplier Data
Immunoprecipitation - Anti-CDK11/CDC2L2/CDK11A antibody (AB19393)
CDK11/CDC2L2/CDK11A was immunoprecipitated from HeLa whole cell lysate with 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab19393 at 1 μg/ml dilution.
Lane 1 : ab19393 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.
All lanes:
Immunoprecipitation - Anti-CDK11/CDC2L2/CDK11A antibody (ab19393)
Predicted band size: 91 kDa
false
- WB
Supplier Data
Western blot - Anti-CDK11/CDC2L2/CDK11A antibody (AB19393)
All lanes:
Western blot - Anti-CDK11/CDC2L2/CDK11A antibody (ab19393) at 1/10000 dilution
Lane 1:
HeLa Whole cell lysate at 15 µg
Lane 2:
HEK293T Whole cell lysate at 15 µg
Predicted band size: 91 kDa
false
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Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to CDK11/CDC2L2/CDK11A immobilized on solid support.Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data
Cyclin-dependent protein kinase that acts as a regulator of transcription and pre-mRNA splicing (PubMed : 12501247, PubMed : 32367068, PubMed : 36104565, PubMed : 40858114). Acts as a key regulator of pre-mRNA splicing by mediating phosphorylation of SF3B1, enabling the association between SF3B1 and U5 and U6 snRNAs in the activated spliceosome, thereby promoting spliceosome assembly (PubMed : 36104565, PubMed : 38059508). Also acts as a regulator of transcription by phosphorylating 'Ser-2' of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 32367068, PubMed : 40858114). Involved in replication-dependent transcription of histone genes : binds to histone genes and phosphorylates POLR2A at 'Ser-2' of the CTD to specifically control transcriptional elongation of histones and recruitment of 3'-end processing factors (PubMed : 32367068). Part of a transcription checkpoint upstream of CDK9, which regulates promoter-proximal pausing by RNA polymerase II, a transcription halt following transcription initiation, but prior to elongation (PubMed : 40858114). Probably regulates promoter-proximal pausing by mediating phosphorylation of POLR2A at 'Ser-2' of the CTD (PubMed : 40858114).. Isoform SV6. Isoform expressed in a non-cell cycle-dependent manner.. Isoform 4. Isoform specifically expressed during the G2-M phases of the cell cycle (PubMed : 12082095). Phosphorylates 'Ser-2' of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 38019613). Promotes centromeric transcription to maintain centromeric cohesion during mitosis (PubMed : 24436071, PubMed : 38019613).
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
Molecular biology of the cell 35:ar18 PubMed38019613
2023
CDK11 facilitates centromeric transcription to maintain centromeric cohesion during mitosis.
Applications
Unspecified application
Species
Unspecified reactive species
Qian Zhang,Yujue Chen,Zhen Teng,Zhen Lin,Hong Liu
Structure (London, England : 1993) 30:1615-1625.e4 PubMed36327972
2022
Crystal structure of the CDK11 kinase domain bound to the small-molecule inhibitor OTS964.
Applications
Unspecified application
Species
Unspecified reactive species
Susan Kelso,Siobhan O'Brien,Igor Kurinov,Stephane Angers,Frank Sicheri
Nature 609:829-834 PubMed36104565
2022
CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1.
Applications
Unspecified application
Species
Unspecified reactive species
Milan Hluchý,Pavla Gajdušková,Igor Ruiz de Los Mozos,Michal Rájecký,Michael Kluge,Benedict-Tilman Berger,Zuzana Slabá,David Potěšil,Elena Weiß,Jernej Ule,Zbyněk Zdráhal,Stefan Knapp,Kamil Paruch,Caroline C Friedel,Dalibor Blazek
Cellular and molecular life sciences : CMLS 77:4693-4708 PubMed32030451
2020
A cyclin-dependent kinase, CDK11/p58, represses cap-dependent translation during mitosis.
Applications
Unspecified application
Species
Unspecified reactive species
Sihyeon An,Oh Sung Kwon,Jinbae Yu,Sung Key Jang
The Journal of biological chemistry 294:18639-18649 PubMed31653703
2019
CDK11-cyclin L1β regulates abscission site assembly.
Applications
Unspecified application
Species
Unspecified reactive species
Matthew J Renshaw,Thomas C Panagiotou,Brigitte D Lavoie,Andrew Wilde
Leukemia 27:578-85 PubMed23044487
2012
GCN5 acetylates and regulates the stability of the oncoprotein E2A-PBX1 in acute lymphoblastic leukemia.
Applications
Unspecified application
Species
Unspecified reactive species
T Holmlund,M J Lindberg,D Grander,A E Wallberg
Free radical biology & medicine 53:1838-47 PubMed23000060
2012
Manganese superoxide dismutase interacts with a large scale of cellular and mitochondrial proteins in low-dose radiation-induced adaptive radioprotection.
Applications
WB
Species
Unspecified reactive species
Angela Eldridge,Ming Fan,Gayle Woloschak,David J Grdina,Brett A Chromy,Jian Jian Li
Cell cycle (Georgetown, Tex.) 10:3706-13 PubMed22037210
2011
Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11(p58) in mouse embryonic stem cells.
Applications
Unspecified application
Species
Unspecified reactive species
Satona Ohno,Masaki Shibayama,Mitsuharu Sato,Akinori Tokunaga,Nobuaki Yoshida
Autophagy 7:1295-301 PubMed21808150
2011
The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy.
Applications
WB
Species
Unspecified reactive species
Simon Wilkinson,Daniel R Croft,Jim O'Prey,Arenda Meedendorp,Margaret O'Prey,Christine Dufès,Kevin M Ryan
Nature 456:971-5 PubMed19011615
2008
Suppression of Myc oncogenic activity by ribosomal protein haploinsufficiency.
Applications
WB
Species
Mouse
Maria Barna,Aya Pusic,Ornella Zollo,Maria Costa,Nadya Kondrashov,Eduardo Rego,Pulivarthi H Rao,Davide Ruggero
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