Anti-CDK12 antibody (ab246887) is a rabbit polyclonal antibody that is used to detect CDK12 in IHC-P, ICC/IF. Suitable for Human samples.
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- Nature 619:184-1922023Heritable transcriptional defects from aberrations of nuclear architecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStamatis Papathanasiou,Nikos A Mynhier,Shiwei Liu,Gregory Brunette,Ema Stokasimov,Etai Jacob,Lanting Li,Caroline Comenho,Bas van Steensel,Jason D Buenrostro,Cheng-Zhong Zhang,David PellmanPubMed 37286600
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CDK12 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NYV4
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Target data
Function
Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
Alternative names
CRK7, CRKRS, KIAA0904, CDK12, Cyclin-dependent kinase 12, Cell division cycle 2-related protein kinase 7, Cell division protein kinase 12, CrkRS, CDC2-related protein kinase 7, hCDK12
Recommended products
Anti-CDK12 antibody (ab246887) is a rabbit polyclonal antibody that is used to detect CDK12 in IHC-P, ICC/IF. Suitable for Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CDK12 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NYV4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CDK12 antibody (ab246887) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (ab246887)
Formalin-fixed, paraffin-embedded human endometrium tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-CDK12 antibody (ab246887)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CDK12 (green) using ab246887 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (ab246887)
Formalin-fixed, paraffin-embedded human testis tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (ab246887)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (ab246887)
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.
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