AB246887

Anti-CDK12 antibody

Name: Anti-CDK12 antibody (ab246887) | Abcam
Brand: Abcam Limited
SKU: AB246887
Price: 560 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Anti-CDK12 antibody (ab246887) is a rabbit polyclonal antibody detecting CDK12 in IHC-P, ICC/IF. Suitable for Human.

View Alternative Names

CRK7, CRKRS, KIAA0904, CDK12, Cyclin-dependent kinase 12, Cell division cycle 2-related protein kinase 7, Cell division protein kinase 12, CrkRS, CDC2-related protein kinase 7, hCDK12

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CDK12 antibody (AB246887)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDK12 antibody (AB246887)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CDK12 (green) using ab246887 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Formalin-fixed, paraffin-embedded human testis tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for CDK12 with ab246887 at a 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CDK12 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NYV4

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-CDK12 antibody (ab246887) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

See full target information CDK12

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101758 PubMed39368479

2024

CDK12 loss drives prostate cancer progression, transcription-replication conflicts, and synthetic lethality with paralog CDK13.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Ching-Yi Tien,Jie Luo,Yu Chang,Yuping Zhang,Yunhui Cheng,Xiaoju Wang,Jianzhang Yang,Rahul Mannan,Somnath Mahapatra,Palak Shah,Xiao-Ming Wang,Abigail J Todd,Sanjana Eyunni,Caleb Cheng,Ryan J Rebernick,Lanbo Xiao,Yi Bao,James Neiswender,Rachel Brough,Stephen J Pettitt,Xuhong Cao,Stephanie J Miner,Licheng Zhou,Yi-Mi Wu,Estefania Labanca,Yuzhuo Wang,Abhijit Parolia,Marcin Cieslik,Dan R Robinson,Zhen Wang,Felix Y Feng,Jonathan Chou,Christopher J Lord,Ke Ding,Arul M Chinnaiyan

International journal of biological sciences 20:1669-1687 PubMed38481813

2024

Tubule-specific cyclin-dependent kinase 12 knockdown potentiates kidney injury through transcriptional elongation defects.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lin Zhang,Tao-Tao Tang,Wei-Jie Ni,Zhong-Tang Li,Liang-Yun-Zi Jiang,Yao Wang,Xuan Zhou,Jing-Yuan Cao,Qing Yin,Wei Jiang,Ya-Jie Zhao,Wei-Hua Gan,Ai-Qing Zhang,Zuo-Lin Li,Yi Wen,Lin-Li Lv,Bi-Cheng Liu,Bin Wang

International journal of oncology 64: PubMed38186306

2024

CDK12 regulates angiogenesis of advanced prostate cancer by IGFBP3.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhong,Wenwu Luo,Nan Li,Xin Tan,Yuqing Li,Shiyuan Yin,Yuhang Huang,Linna Fang,Wei Ma,Yongping Cai,Yu Yin

Nature 619:184-192 PubMed37286600

2023

Heritable transcriptional defects from aberrations of nuclear architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Stamatis Papathanasiou,Nikos A Mynhier,Shiwei Liu,Gregory Brunette,Ema Stokasimov,Etai Jacob,Lanting Li,Caroline Comenho,Bas van Steensel,Jason D Buenrostro,Cheng-Zhong Zhang,David Pellman

International journal of molecular sciences 24: PubMed36613882

2022

Ladinin 1 Shortens Survival via Promoting Proliferation and Enhancing Invasiveness in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yuan Chang,Yung-Chi Huang,Hung-Hsing Chiang,Yu-Yuan Wu,Kuan-Li Wu,Yung-Yun Chang,Lian-Xiu Liu,Ying-Ming Tsai,Ya-Ling Hsu

Theranostics 12:4753-4766 PubMed35832084

2022

MiR-155 deficiency protects renal tubular epithelial cells from telomeric and genomic DNA damage in cisplatin-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Yin,Ya-Jie Zhao,Wei-Jie Ni,Tao-Tao Tang,Yao Wang,Jing-Yuan Cao,Di Yin,Yi Wen,Zuo-Lin Li,Yi-Lin Zhang,Wei Jiang,Yue Zhang,Xiao-Yu Lu,Ai-Qing Zhang,Wei-Hua Gan,Lin-Li Lv,Bi-Cheng Liu,Bin Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDK12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Immunocytochemistry/ Immunofluorescence - Anti-CDK12 antibody (AB246887)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK12 antibody (AB246887)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise