Anti-CDK13 antibody [EPR29353-534] (ab323865)

Rabbit Recombinant Monoclonal CDK13 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (AB323865), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB
Human	Tested	Tested
Mouse	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Target data

See full target information CDK13

Function

Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis. In case of infection by HIV-1 virus, interacts with HIV-1 Tat protein acetylated at 'Lys-50' and 'Lys-51', thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein Nef.

Additional Targets

Cdk13

Alternative names

CDC2L, CDC2L5, CHED, KIAA1791, CDK13, Cyclin-dependent kinase 13, CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, Cell division protein kinase 13, Cholinesterase-related cell division controller, hCDK13

Rabbit Recombinant Monoclonal CDK13 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR29353-534
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis tissue using rabbit Anti-CDK13 antibody
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling CDK13 with ab323865 at 1/1000 (0.52 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human breast carcinoma tissue using rabbit Anti-CDK13 antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling CDK13 with ab323865 at 1/100 (5.2 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human breast carcinoma (PMID: 33292020).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum tissue using rabbit Anti-CDK13 antibody
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CDK13 with ab323865 at 1/100 (5.2 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Western blot staining using rabbit Anti-CDK13 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
To minimize protein degradation, the blot was lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
Exposure time: Lane1-2: 26 seconds, lane 3: 48 seconds
Loading control: Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) 1:10000 (124Kda).
All lanes: Western blot - Anti-CDK13 antibody [EPR29353-534] (ab323865) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 200 kDa, 124 kDa
Exposure time: 10s
Western blot - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Western blot staining using rabbit Anti-CDK13 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16721827).
In Western blot, Anti-alpha Actinin 4 antibody [EPR2533 (2)] - Loading Control (Anti-alpha Actinin 4 antibody [EPR2533(2)] ab108198) staining at 1/200000 dilution (104 kDa).
All lanes: Western blot - Anti-CDK13 antibody [EPR29353-534] (ab323865) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeting CDK13 whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 200 kDa, 104 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse breast cancer tissue using rabbit Anti-CDK13 antibody
Immunohistochemical analysis of paraffin-embedded mouse breast cancer tissue labeling CDK13 with ab323865 at 1/1000 (0.52 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse breast cancer.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse lung tissue using rabbit Anti-CDK13 antibody
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CDK13 with ab323865 at 1/1000 (0.52 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse lung (PMID: 31440507).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum tissue using rabbit Anti-CDK13 antibody
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CDK13 with ab323865 at 1/1000 (0.52 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK13 antibody [EPR29353-534] (ab323865)
CDK13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil tissue using rabbit Anti-CDK13 antibody
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CDK13 with ab323865 at 1/100 (5.2 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins