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AB68265

Anti-CDK2 (phospho T14) antibody [EP2234Y]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • 20ul selling size
  • What is this?

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(13 Publications)

Rabbit Recombinant Monoclonal CDK2 phospho T14 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Chinese hamster samples. Cited in 13 publications.

View Alternative Names

CDKN2, CDK2, Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CDK2 with Purified 68265 at 1 : 1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CDK2 with Purified 68265 at 1 : 1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CDK2 with Purified 68265 at 1 : 1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)
  • WB

Unknown

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)

All lanes:

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

false

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)
  • WB

Unknown

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)

All lanes:

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution

Lane 1:

Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 4:

Untreated C2C12 (Mouse myoblasts myoblast) whole cell lysate at 15 µg

Lane 5:

C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate at 15 µg

Lane 6:

C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

false

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)
  • WB

Unknown

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (AB68265)

All lanes:

Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution

Lane 1:

CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate

Lane 2:

CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

false

  • Carrier free

    Anti-CDK2 (phospho T14) antibody [EP2234Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2234Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Chinese hamster

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects Cdk2 phosphorylated on Threonine 14.

The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p>The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p>The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p>The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chinese hamster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis (PubMed : 10499802, PubMed : 10884347, PubMed : 10995386, PubMed : 10995387, PubMed : 11051553, PubMed : 11113184, PubMed : 12944431, PubMed : 15800615, PubMed : 17495531, PubMed : 19966300, PubMed : 20935635, PubMed : 21262353, PubMed : 21596315, PubMed : 28216226, PubMed : 28666995). Phosphorylates CABLES1, CTNNB1, CDK2AP2, ERCC6, NBN, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, SUV39H1, EZH2 (PubMed : 10499802, PubMed : 10995386, PubMed : 10995387, PubMed : 11051553, PubMed : 11113184, PubMed : 12944431, PubMed : 15800615, PubMed : 19966300, PubMed : 20935635, PubMed : 21262353, PubMed : 21596315, PubMed : 24728993, PubMed : 28216226). Triggers duplication of centrosomes and DNA (PubMed : 11051553). Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus (PubMed : 18372919, PubMed : 19238148, PubMed : 19561645). Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in embryonic stem cells (ESCs) (PubMed : 18372919, PubMed : 19238148, PubMed : 19561645). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase (PubMed : 18372919, PubMed : 19238148, PubMed : 19561645). EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing (PubMed : 20935635). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC (PubMed : 19966300). Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis (PubMed : 15800615, PubMed : 20195506, PubMed : 21319273). In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation (PubMed : 15800615). Involved in regulation of telomere repair by mediating phosphorylation of NBN (PubMed : 28216226). Phosphorylation of RB1 disturbs its interaction with E2F1 (PubMed : 10499802). NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication (PubMed : 11051553). Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase (PubMed : 10995386, PubMed : 10995387). Required for vitamin D-mediated growth inhibition by being itself inactivated (PubMed : 20147522). Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner (PubMed : 20079829). USP37 is activated by phosphorylation and thus triggers G1-S transition (PubMed : 21596315). CTNNB1 phosphorylation regulates insulin internalization (PubMed : 21262353). Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed : 29203878). Acts as a regulator of the phosphatidylinositol 3-kinase/protein kinase B signal transduction by mediating phosphorylation of the C-terminus of protein kinase B (PKB/AKT1 and PKB/AKT2), promoting its activation (PubMed : 24670654).
See full target information CDK2 phospho T14
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 89:104451 PubMed36738481

2023

VPS35 promotes cell proliferation via EGFR recycling and enhances EGFR inhibitors response in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junxian Yu,Haoran Feng,Qingqing Sang,Fangyuan Li,Mengdi Chen,Beiqin Yu,Zhuoqing Xu,Tao Pan,Xiongyan Wu,Junyi Hou,Zhenggang Zhu,Chao Yan,Liping Su,Jianfang Li,Bingya Liu

OncoTargets and therapy 13:3595-3605 PubMed32425555

2020

Phosphoproteomics Reveals Key Regulatory Kinases and Modulated Pathways Associated with Ovarian Cancer Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Hu,Lejia Sun,Yinglan Zhang,Jinghe Lang,Jun Rao

International journal of cancer 147:1474-1484 PubMed32159854

2020

Adefovir dipivoxil induces DNA replication stress and augments ATR inhibitor-related cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Agata Patel,Elena Seraia,Daniel Ebner,Anderson Joseph Ryan

European journal of pharmacology 863:172658 PubMed31518562

2019

Flavopereirine induces cell cycle arrest and apoptosis via the AKT/p38 MAPK/ERK1/2 signaling pathway in human breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hsuan-Te Yeh,Yi-Sheng Tsai,Ming-Shan Chen,Yi-Zhen Li,Wen-Chun Lin,Ying-Ray Lee,Ya-Shih Tseng,Shew-Meei Sheu

Cellular signalling 54:10-16 PubMed30468767

2018

Regulation of the stability and activity of CDC25A and CDC25B by protein phosphatase PP2A and 14-3-3 binding.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri Kohama,Megumi Saito,Mizue Yada,Hiroshi Sakurai

American journal of physiology. Regulatory, integr 315:R461-R468 PubMed29718700

2018

Relationship between intermuscular adipose tissue infiltration and myostatin before and after aerobic exercise training.

Applications

Unspecified application

Species

Unspecified reactive species

Adam R Konopka,Christopher A Wolff,Miranda K Suer,Matthew P Harber

Molecular medicine reports 17:2572-2580 PubMed29207130

2017

Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53‑independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Gyeong Lim,Guen Tae Kim,Bo Min Kim,Eun Ji Kim,Sang-Yong Kim,Young Min Kim

Cell cycle (Georgetown, Tex.) 16:2058-2072 PubMed28880708

2017

Inhibition of E2F1 activity and cell cycle progression by arsenic via retinoblastoma protein.

Applications

Unspecified application

Species

Unspecified reactive species

Lynn A Sheldon

Oncotarget 7:85365-85380 PubMed27863419

2016

TSC loss distorts DNA replication programme and sensitises cells to genotoxic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Govind M Pai,Alexandra Zielinski,Dennis Koalick,Kristin Ludwig,Zhao-Qi Wang,Kerstin Borgmann,Helmut Pospiech,Ignacio Rubio

Cell cycle (Georgetown, Tex.) 12:3770-80 PubMed24107632

2013

SMG-1 suppresses CDK2 and tumor growth by regulating both the p53 and Cdc25A signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Evgenia Gubanova,Natalia Issaeva,Camilla Gokturk,Tatjana Djureinovic,Thomas Helleday
View all publications
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