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AB68266

Anti-Cdk4 antibody [EPR2513Y]

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(29 Publications)

Rabbit Recombinant Monoclonal CDK4 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 29 publications.

View Alternative Names

Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR2513Y] (AB68266)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR2513Y] (AB68266)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cdk4 with ab68266 at 1 : 100 (5.4 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain.

PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-Cdk4 antibody [EPR2513Y] (AB68266)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cdk4 antibody [EPR2513Y] (AB68266)

Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling Cdk4 with ab68266 at 1/50 dilution (1μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunoprecipitation - Anti-Cdk4 antibody [EPR2513Y] (AB68266)
  • IP

Unknown

Immunoprecipitation - Anti-Cdk4 antibody [EPR2513Y] (AB68266)

ab68266 at 1 : 30 dilution (2 μg) immunoprecipitating Cdk4 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
Lane 1 (input) : K562 whole cell lysate 10 μg
Lane 2 (+) : ab68266 & K562 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab68266 in K562 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cdk4 antibody [EPR2513Y] (ab68266)

Predicted band size: 34 kDa

false

Western blot - Anti-Cdk4 antibody [EPR2513Y] (AB68266)
  • WB

Lab

Western blot - Anti-Cdk4 antibody [EPR2513Y] (AB68266)

Exposure times : Lane 1-3 : 30 secs; Lane 4-5 : 180 secs.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cdk4 antibody [EPR2513Y] (ab68266) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 3:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 4:

Rat lung lysate at 20 µg

Lane 5:

Mouse lung lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cdk4 antibody [EPR2513Y]

  • 578 PE

    PE Anti-Cdk4 antibody [EPR2513Y]

  • Carrier free

    Anti-Cdk4 antibody [EPR2513Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2513Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdk4 also known as Cyclin-dependent kinase 4 is a serine/threonine kinase involved in cell cycle regulation. Cdk4 has a molecular weight of approximately 34 kDa. This protein mainly locates in the nucleus of the cell and its expression occurs in diverse tissues. Cdk4 partners with D-type cyclins to exert its function which is essential for the transition from the G1 phase to the S phase of the cell cycle. Researchers often study Cdk4 using techniques like immunohistochemistry (IHC) to determine its expression and localization in different tissues.
Biological function summary

Cdk4 plays a fundamental role in cell cycle regulation by forming a complex with D-type cyclins. Together they phosphorylate the retinoblastoma protein (Rb) resulting in the release of E2F transcription factors which promote the expression of genes necessary for DNA replication. The Cdk4/cyclin D complex regulates the cell's commitment to enter S phase and continue cell division. This regulation is key for normal cell proliferation and tissue homeostasis. Cdk4 activity is strictly controlled by INK4 family members acting as inhibitors and adding an extra regulation layer.

Pathways

Cdk4 is an integral part of important signaling pathways like the cell cycle and PI3K/AKT pathways. In the cell cycle pathway Cdk4 relays signals downstream that drive the transition from the G1 to S phase through its interaction with cyclin D and Rb. In the PI3K/AKT pathway signaling can influence cyclin D expression indirectly affecting Cdk4 activity. Proteins such as Cdk6 closely relate to Cdk4 and often compensate or partner with Cdk4 in these pathways to maintain proper cell cycle progression.

Abnormalities in Cdk4 function or regulation relate to cancer and certain metabolic syndromes. Hyperactivity of Cdk4 due to overexpression or mutation frequently occurs in several cancers including melanoma and breast cancer. In these malignancies Cdk4 aberrantly drives excessive cell proliferation. Mutations or alterations in proteins such as p16 an inhibitor of Cdk4 are often found in these cancers highlighting the critical role of Cdk4 in disease progression. Understanding the role of Cdk4 allows researchers to develop targeted therapies such as Cdk4/6 inhibitors offering new treatment options.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
See full target information CDK4
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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108247 PubMed39894218

2025

A BRAF-activated noncoding RNA attenuates clear cell renal cell carcinoma via repression of glucose-6-phosphate dehydrogenase.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Liu,Yueli Ni,Honggang Bai,Xiangjie Liu,Asif Shahzad,Kun Cui,Qiuxin Duan,Ziyuan Bai,Yurong Dong,Zihan Yi,Buqing Sai,Yingmin Kuang,Chen Guo,Yuechun Zhu,Qiao Zhang,Zhe Yang

Molecular therapy. Nucleic acids 36:102400 PubMed39759876

2025

A sort and sequence approach to dissect heterogeneity of response to a self-amplifying RNA vector in a novel human muscle cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel D Barton,John S Tregoning,Ziyin Wang,Daniel Gonçalves-Carneiro,Radhika Patel,Paul F McKay,Robin J Shattock

Journal of cellular physiology 239:e31330 PubMed38828927

2024

LncRNA MEG3 regulates ferroptosis of lens epithelial cells via PTBP1/GPX4 axis to participate in age-related cataract.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyuan Zhang,Chuanfei Zheng,Jiuhong Zhao,Xiaoming Xu,Jun Yao

Nature communications 14:6699 PubMed37872167

2023

Broad-spectrum kinome profiling identifies CDK6 upregulation as a driver of lenvatinib resistance in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Oi Ning Leung,Yang Yang,Rainbow Wing Hei Leung,Karl Kam Hei So,Hai Jun Guo,Martina Mang Leng Lei,Gregory Kenneth Muliawan,Yuan Gao,Qian Qian Yu,Jing Ping Yun,Stephanie Ma,Qian Zhao,Terence Kin Wah Lee

Islets 15:2219105 PubMed37265106

2023

7-Ketocholesterol accelerates pancreatic β-cell senescence by inhibiting the SIRT1/CDK4-Rb-E2F1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuping Zhu,Wei Wang,Nan Wu,Sunyue He,Xihua Lin,Wenjing Zhang,Jiaqiang Zhou

American journal of translational research 14:8539-8551 PubMed36628219

2022

Sanguinarine promotes apoptosis of hepatocellular carcinoma cells via regulating the miR-497-5p/CDK4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Quanhua Ding,Weina Zhu,Shenghao Zhu,Xinfeng Zhou

NPJ breast cancer 8:126 PubMed36446794

2022

Crystal structure of active CDK4-cyclin D and mechanistic basis for abemaciclib efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Severine Isabelle Gharbi,Laura A Pelletier,Alfonso Espada,Jesus Gutiérrez,Sonia Maria Gutiérrez Sanfeliciano,Charles T Rauch,Maria Patricia Ganado,Carmen Baquero,Elisabet Zapatero,Aiping Zhang,Jordi Benach,Anna-Maria Russell,Leticia Cano,Sandra Gomez,Howard Broughton,Nicholas Pulliam,Carmen Maria Perez,Raquel Torres,Marjoke F Debets,Alfonso de Dios,Oscar Puig,Mark T Hilgers,Maria Jose Lallena

Bioengineered 13:9411-9424 PubMed35412947

2022

Long non-coding RNA-PCGEM1 contributes to prostate cancer progression by sponging microRNA miR-129-5p to enhance chromatin licensing and DNA replication factor 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiao Fu,Fangfang Wang,Jun Yang,Wei Sun,Zhi Hu,Lv Xu,Hao Chu,Xiao Wang,Wei Zhang

ACS omega 6:32253-32261 PubMed34870045

2021

Gallium-68 Labeling of the Cyclin-Dependent Kinase 4/6 Inhibitors as Positron Emission Tomography Radiotracers for Tumor Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Liu,Ziyi Yang,Mingyu Liu,Xiangwei Wang,Shaoli Song,Xiaoping Xu,Zhongyi Yang

Virus research 302:198469 PubMed34090962

2021

The folate antagonist methotrexate diminishes replication of the coronavirus SARS-CoV-2 and enhances the antiviral efficacy of remdesivir in cell culture models.

Applications

Unspecified application

Species

Unspecified reactive species

Kim M Stegmann,Antje Dickmanns,Sabrina Gerber,Vella Nikolova,Luisa Klemke,Valentina Manzini,Denise Schlösser,Cathrin Bierwirth,Julia Freund,Maren Sitte,Raimond Lugert,Gabriela Salinas,Toni Luise Meister,Stephanie Pfaender,Dirk Görlich,Bernd Wollnik,Uwe Groß,Matthias Dobbelstein
View all publications
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