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Rabbit Recombinant Monoclonal CDK4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.


Images

Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (AB108355), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR4513-54-3] (AB108355), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR4513-54-3] (AB108355), expandable thumbnail
  • Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (AB108355), expandable thumbnail
  • Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (AB108355), expandable thumbnail

Publications

  • Endocrine journal 69:1117-11292022
    Overexpression of lncRNA HCP5 in human umbilical cord mesenchymal stem cell-derived exosomes promoted the proliferation and inhibited the apoptosis of ovarian granulosa cells via the musashi RNA-binding protein 2/oestrogen receptor alpha 1 axis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yu-Ting Sun,Jun-Hong Cai,Shan Bao
    PubMed 35545536
  • Molecular medicine reports 24:2021
    Overexpression of EphB4 promotes neurogenesis, but inhibits neuroinflammation in mice with acute ischemic stroke.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Jin Wang,Zun Zhang,Shaojing Fu,Xiaojie Li,Xinhui Li,Shaobin Wang,Lihe Yuan
    PubMed 34476505

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

This product gave a positive signal in HAP1 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Associated Products

Select an associated product type

14 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

      Alternative names

      Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

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      Rabbit Recombinant Monoclonal CDK4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

      Alternative names

      Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR4513-54-3

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Stable for 12 months at -20°C

      Notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      Cdk4 also known as Cyclin-dependent kinase 4 is a serine/threonine kinase involved in cell cycle regulation. Cdk4 has a molecular weight of approximately 34 kDa. This protein mainly locates in the nucleus of the cell and its expression occurs in diverse tissues. Cdk4 partners with D-type cyclins to exert its function which is essential for the transition from the G1 phase to the S phase of the cell cycle. Researchers often study Cdk4 using techniques like immunohistochemistry (IHC) to determine its expression and localization in different tissues.

      Biological function summary

      Cdk4 plays a fundamental role in cell cycle regulation by forming a complex with D-type cyclins. Together they phosphorylate the retinoblastoma protein (Rb) resulting in the release of E2F transcription factors which promote the expression of genes necessary for DNA replication. The Cdk4/cyclin D complex regulates the cell's commitment to enter S phase and continue cell division. This regulation is key for normal cell proliferation and tissue homeostasis. Cdk4 activity is strictly controlled by INK4 family members acting as inhibitors and adding an extra regulation layer.

      Pathways

      Cdk4 is an integral part of important signaling pathways like the cell cycle and PI3K/AKT pathways. In the cell cycle pathway Cdk4 relays signals downstream that drive the transition from the G1 to S phase through its interaction with cyclin D and Rb. In the PI3K/AKT pathway signaling can influence cyclin D expression indirectly affecting Cdk4 activity. Proteins such as Cdk6 closely relate to Cdk4 and often compensate or partner with Cdk4 in these pathways to maintain proper cell cycle progression.

      Associated diseases and disorders

      Abnormalities in Cdk4 function or regulation relate to cancer and certain metabolic syndromes. Hyperactivity of Cdk4 due to overexpression or mutation frequently occurs in several cancers including melanoma and breast cancer. In these malignancies Cdk4 aberrantly drives excessive cell proliferation. Mutations or alterations in proteins such as p16 an inhibitor of Cdk4 are often found in these cancers highlighting the critical role of Cdk4 in disease progression. Understanding the role of Cdk4 allows researchers to develop targeted therapies such as Cdk4/6 inhibitors offering new treatment options.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355), expandable thumbnail

        Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355)

        Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 μg)
        Lanes 2, 4 and 6: CDK4 knockout HAP1 cell lysate (20 μg)
        Lanes 1 and 2: Green signal from target - ab108355 observed at 34 kDa
        Lanes 3 and 4: Red signal from loading control - Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 observed at 42 kDa
        Lanes 5 and 6: Merged (red and green) signal
        ab108355 was shown to specifically react with CDK4 when CDK4 knockout samples were used. Wild-type and CDK4 knockout samples were subjected to SDS-PAGE. ab108355 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta actin) were both diluted at 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

        All lanes: Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355)

        Predicted band size: 162 kDa, 34 kDa, 41 kDa, 43 kDa, 51 kDa, 53 kDa, 55 kDa, 68 kDa, 84 kDa, 89 kDa, 93 kDa

        Observed band size: 62 kDa, 69 kDa, 89 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355)

        ab108355 staining Cdk4 in wild-type HAP1 cells (top panel) and Cdk4 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab108355 at 1/400 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1μg/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) (shown in pseudo colour red). Nuclear DNA was labelled in blue with DAPI.

      • Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355)

        Immunofluorescence staining of MCF7 cells with purified ab108355 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108355 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

      • Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355), expandable thumbnail
        This image is courtesy of a customer review submitted by Sonia Rocha

        Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355)

        All lanes: Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355) at 1/1000 dilution

        Lane 1: Human osteosarcoma cells - control, non-targeting siRNA at 20 µg

        Lane 2: Human osteosarcoma cells - siRNA for CDK4 at 20 µg

        Secondary

        All lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 34 kDa

        Observed band size: 34 kDa

        Exposure time: 5s

      • Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355), expandable thumbnail

        Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355)

        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

        All lanes: Western blot - Anti-Cdk4 antibody [EPR4513-54-3] (ab108355) at 1/5000 dilution

        Lane 1: HeLa cell lysate at 10 µg

        Lane 2: K562 cell lysate at 10 µg

        Lane 3: Ramos cell lysate at 10 µg

        Secondary

        All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

        Predicted band size: 34 kDa

        Observed band size: 34 kDa

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      Product protocols

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