JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154537

Anti-CDK5RAP1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal CDK5RAP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDK5RAP1 aa 300-600.

View Alternative Names

C20orf34, CGI-05, HSPC167, CDK5RAP1, Mitochondrial tRNA methylthiotransferase CDK5RAP1, CDK5 activator-binding protein C42, CDK5 regulatory subunit-associated protein 1, mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase, mt-tRNA-N6-(dimethylallyl)adenosine(37) methylthiotransferase

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP1 antibody (AB154537)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP1 antibody (AB154537)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CDK5RAP1 using ab154537 at 1/500 dilution. Right panel costained with Hoechst 33342.

Western blot - Anti-CDK5RAP1 antibody (AB154537)
  • WB

Supplier Data

Western blot - Anti-CDK5RAP1 antibody (AB154537)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CDK5RAP1 antibody (ab154537) at 1/500 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CDK5RAP1 aa 300-600. The exact immunogen used to generate this antibody is proprietary information.

Q96SZ6

style
left-column

Complementary Products

Proteins & Peptides

AB60761

Recombinant human CDK5 + p25 protein

Functional Studies - Recombinant human CDK5 + p25 protein (AB60761)

  • 4
  • 1
Primary Antibodies

AB204376

Anti-WASF2/WAVE2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASF2/WAVE2 antibody (AB204376)

  • 5
  • 1
Primary Antibodies

AB176594

Anti-ZZEF1 antibody

Immunoprecipitation - Anti-ZZEF1 antibody (AB176594)

  • 0
  • 0
Primary Antibodies

AB196562

Anti-UPD antibody

Immunocytochemistry/ Immunofluorescence - Anti-UPD antibody (AB196562)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp) (PubMed : 22422838, PubMed : 25738458, PubMed : 28981754). Essential for efficient and highly accurate protein translation by the ribosome (PubMed : 22422838, PubMed : 25738458, PubMed : 28981754). Specifically inhibits CDK5 activation by CDK5R1 (PubMed : 11882646). Essential for efficient mitochondrial protein synthesis and respiratory chain; shows pathological consequences in mitochondrial disease (PubMed : 25738458).
See full target information CDK5RAP1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 1:e201800070 PubMed30456350

2018

Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Hannelore Hartmann,Daniel Hornburg,Mareike Czuppa,Jakob Bader,Meike Michaelsen,Daniel Farny,Thomas Arzberger,Matthias Mann,Felix Meissner,Dieter Edbauer
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com