Rabbit Polyclonal CDK5RAP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDK5RAP1 aa 300-600.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CDK5RAP1 aa 300-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q96SZ6
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Target data
Function
Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp) (PubMed:22422838, PubMed:25738458, PubMed:28981754). Essential for efficient and highly accurate protein translation by the ribosome (PubMed:22422838, PubMed:25738458, PubMed:28981754). Specifically inhibits CDK5 activation by CDK5R1 (PubMed:11882646). Essential for efficient mitochondrial protein synthesis and respiratory chain; shows pathological consequences in mitochondrial disease (PubMed:25738458).
Alternative names
Mitochondrial tRNA methylthiotransferase CDK5RAP1, CDK5 activator-binding protein C42, CDK5 regulatory subunit-associated protein 1, mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase, mt-tRNA-N6-(dimethylallyl)adenosine(37) methylthiotransferase, HSPC167, CDK5RAP1, C20orf34, CGI-05
Recommended products
Rabbit Polyclonal CDK5RAP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDK5RAP1 aa 300-600.
Alternative names
Mitochondrial tRNA methylthiotransferase CDK5RAP1, CDK5 activator-binding protein C42, CDK5 regulatory subunit-associated protein 1, mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase, mt-tRNA-N6-(dimethylallyl)adenosine(37) methylthiotransferase, HSPC167, CDK5RAP1, C20orf34, CGI-05
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CDK5RAP1 aa 300-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q96SZ6
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-CDK5RAP1 antibody (ab154537)
7.5% SDS PAGE
All lanes: Western blot - Anti-CDK5RAP1 antibody (ab154537) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 68 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP1 antibody (ab154537)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CDK5RAP1 using ab154537 at 1/500 dilution. Right panel costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP1 antibody (ab154537)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma, labeling CDK5RAP1 using ab154537 at 1/250 dilution.
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