AB70213

Anti-CDK5RAP2 antibody

Name: Anti-CDK5RAP2 antibody (ab70213) | Abcam
Brand: Abcam Limited
SKU: AB70213
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CDK5RAP2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CDK5RAP2.

View Alternative Names

CEP215, KIAA1633, CDK5RAP2, CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215

2 Images

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Western blot - Anti-CDK5RAP2 antibody (AB70213)

All lanes:

Western blot - Anti-CDK5RAP2 antibody (ab70213) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 215 kDa

Observed band size: 238 kDa,330 kDa

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Exposure time: 30s

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Immunoprecipitation - Anti-CDK5RAP2 antibody (AB70213)

Detection of Human CDK5RAP2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 1/4 of IP loaded) using affinity purified rabbit anti-CDK5RAP2 antibody ab70213 at 3 µg/mg of lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note : bands between 55 and 71 kDa represent the IgG chains.

All lanes:

Immunoprecipitation - Anti-CDK5RAP2 antibody (ab70213)

Predicted band size: 215 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CDK5RAP2. The exact immunogen used to generate this antibody is proprietary information.

Q96SN8

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "3-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/15000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gamma-TuRC) onto centrosomes (PubMed : 26485573). In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery (PubMed : 29162697). Required for the recruitment of AKAP9 to centrosomes (PubMed : 29162697). Plays a role in neurogenesis (By similarity).

See full target information CDK5RAP2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102658 PubMed36356903

2022

Cartwheel disassembly regulated by CDK1-cyclin B kinase allows human centriole disengagement and licensing.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Huang,Xiaowei Xu,Guangwei Xin,Boyan Zhang,Qing Jiang,Chuanmao Zhang

The Journal of cell biology 220: PubMed33351100

2020

PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations.

Applications

Unspecified application

Species

Unspecified reactive species

Wangfei Chi,Gang Wang,Guangwei Xin,Qing Jiang,Chuanmao Zhang

Journal of cell science 130:3676-3684 PubMed28883092

2017

Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2.

Applications

Unspecified application

Species

Unspecified reactive species

Elsa A Tungadi,Ami Ito,Tomomi Kiyomitsu,Gohta Goshima

Proceedings of the National Academy of Sciences of 113:9304-9 PubMed27486244

2016

Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins.

Applications

WB, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

Igor Cervenka,Jana Valnohova,Ondrej Bernatik,Jakub Harnos,Matej Radsetoulal,Katerina Sedova,Katerina Hanakova,David Potesil,Miroslava Sedlackova,Alena Salasova,Zachary Steinhart,Stephane Angers,Gunnar Schulte,Ales Hampl,Zbynek Zdrahal,Vitezslav Bryja

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDK5RAP2 antibody

Western blot - Anti-CDK5RAP2 antibody (AB70213)

Immunoprecipitation - Anti-CDK5RAP2 antibody (AB70213)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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