AB327115

Anti-CDK5RAP2 Antibody [CL3392]

Name: Anti-CDK5RAP2 Antibody [CL3392] (ab327115) | Abcam
Brand: Abcam Limited
SKU: AB327115
Price: 600 USD
Availability: InStock

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Mouse Monoclonal CDK5RAP2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDK5RAP2.

View Alternative Names

CEP215, KIAA1633, CDK5RAP2, CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunofluorescence staining of U251 cells using ab327115 at 2–10 µg/ml, showing specific staining of the centrosome in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunofluorescence staining of U2OS cells using ab327115 at 2–10 µg/ml, showing specific staining of the centrosome in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunofluorescence staining of A431 cells using ab327115 at 2–10 µg/ml, showing specific staining of the centrosome in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunofluorescence staining of HeLa cells using ab327115 at 2–10 µg/ml, showing specific staining of the centrosome in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunohistochemical staining of human kidney using ab327115 at 1 : 200–1 : 500 dilution, showing centrosome-like positivity in renal cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunohistochemical staining of human testis using ab327115 at 1 : 200–1 : 500 dilution, showing centrosome-like immunoreactivity in a subset of seminiferous epithelium cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunohistochemical staining of human tonsil using ab327115 at 1 : 200–1 : 500 dilution, showing centrosome-like positivity in lymphoid cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP2 Antibody [CL3392] (AB327115)

Immunohistochemical staining of human breast cancer using ab327115 at 1 : 200–1 : 500 dilution, showing centrosome-like immunoreactivity in tumor cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3392

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CDK5RAP2.

Q96SN8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Potential regulator of CDK5 activity via its interaction with CDK5R1 (PubMed : 15164053). Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter (PubMed : 19282672). Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends (PubMed : 18042621, PubMed : 17959831, PubMed : 19553473). Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gTuRC) onto centrosomes (PubMed : 18042621, PubMed : 17959831, PubMed : 26485573, PubMed : 39321809). In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery (PubMed : 29162697). Required for the recruitment of AKAP9 to centrosomes (PubMed : 29162697). Plays a role in neurogenesis (By similarity).

See full target information CDK5RAP2

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Product promise

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