Anti-CDK5RAP2 antibody [RP23040190]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 antibody [RP23040190] (AB313477)

ImmunoFluorscent analysis of HeLa cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous CDK5RAP2 with ab313477 at 2 µg/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of CDK5RAP2 (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating specific localization of CDK5RAP2 in the centrosomes. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-CDK5RAP2 Antibody [RP23040190] (AB313477)

All lanes:

Western blot - Anti-CDK5RAP2 Antibody [RP23040190] (ab313477) at 1 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

A549 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 215 kDa

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