Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (AB249450)

This data was developed using ab168353, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution.

• WB

Unknown

Western blot - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (AB249450)

This data was developed using ab168353, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CDK5RAP3 antibody [EPR11293] (<a href='/en-us/products/primary-antibodies/cdk5rap3-antibody-epr11293-ab168353'>ab168353</a>) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from 293T cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 57 kDa

false

• WB

Unknown

Western blot - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (AB249450)

This data was developed using ab168353, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CDK5RAP3 antibody [EPR11293] (<a href='/en-us/products/primary-antibodies/cdk5rap3-antibody-epr11293-ab168353'>ab168353</a>) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

Lane 4:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

false

• WB

Lab

Western blot - Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (AB249450)

This data was developed using ab168353, the same antibody clone in a different buffer formulation.

Western blot : Anti-CDK5RAP3 antibody [EPR11293] ab168353 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 62 kDa in Wild-type A549 cell lysates with no signal observed at this size in CDK5RAP3 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CDK5RAP3 antibody [EPR11293] (<a href='/en-us/products/primary-antibodies/cdk5rap3-antibody-epr11293-ab168353'>ab168353</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CDK5RAP3 knockout A549 at 20 µg

Lane 3:

Calu-3 at 20 µg

Lane 4:

SK-N-SH at 20 µg

Lane 5:

SK-OV-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 62 kDa,37 kDa

true

Exposure time: 30s