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Rabbit Recombinant Monoclonal CDK5RAP3 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 6 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203), expandable thumbnail
  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203), expandable thumbnail
  • Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203), expandable thumbnail
  • Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203), expandable thumbnail
  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Expected
Expected
Tested
Not recommended
Expected

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Substrate adapter of E3 ligase complexes mediating ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, and which is involved in various processes, such as ribosome recycling and reticulophagy (also called ER-phagy) (PubMed:23152784, PubMed:30635284, PubMed:32851973, PubMed:36121123, PubMed:36543799, PubMed:37595036, PubMed:38383785, PubMed:38383789). As part of the UREL complex, plays a key role in ribosome recycling by promoting mono-ufmylation of RPL26/uL24 subunit of the 60S ribosome (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 occurs on free 60S ribosomes following ribosome dissociation: it weakens the junction between post-termination 60S subunits and SEC61 translocons, promoting release and recycling of the large ribosomal subunit from the endoplasmic reticulum membrane (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 and subsequent 60S ribosome recycling either take place after normal termination of translation or after ribosome stalling during cotranslational translocation at the endoplasmic reticulum (PubMed:32851973, PubMed:37595036, PubMed:38383785, PubMed:38383789). Within the UREL complex, CDK5RAP3 acts as a substrate adapter that constrains UFL1 ligase activity to mono-ufmylate RPL26/uL24 at 'Lys-134' (PubMed:36121123, PubMed:38383785, PubMed:38383789). The UREL complex is also involved in reticulophagy in response to endoplasmic reticulum stress by promoting ufmylation of proteins such as CYB5R3, thereby promoting lysosomal degradation of ufmylated proteins (PubMed:36543799). Also acts as a regulator of transcription: negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922). May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629). Required for liver development (By similarity).(Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CDK5RAP3 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 6 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR11294(B)

Purification technique

Affinity purification Protein A

Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab157203 & HEK-293 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab157203 in HEK-293 whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    Predicted band size: 57 kDa

  • Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    All lanes: Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/2000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa

    Observed band size: 57 kDa

  • Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    All lanes: Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution

    Lane 1: CTLL-2 (Mouse T lymphocyte) whole cell lysates at 20 µg

    Lane 2: EL4 (Mouse lymphoma T lymphocyte) whole cell lysates at 20 µg

    Lane 3: H9 (Human lymphoma T lymphocyte) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa

    Observed band size: 57 kDa

  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.

    All lanes: Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    Predicted band size: 57 kDa

  • Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    All lanes: Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

    Lane 3: A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

    Lane 4: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

    Predicted band size: 57 kDa

  • Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

    Intracellular Flow Cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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