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AB157203

Anti-CDK5RAP3 antibody [EPR11294(B)]

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(10 Publications)

Rabbit Recombinant Monoclonal CDK5RAP3 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 10 publications.

View Alternative Names

IC53, LZAP, MSTP016, OK/SW-cl.114, PP1553, CDK5RAP3, CDK5 regulatory subunit-associated protein 3, CDK5 activator-binding protein C53, LXXLL/leucine-zipper-containing ARF-binding protein, Protein HSF-27

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

Intracellular Flow Cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • IP

Unknown

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Predicted band size: 57 kDa

false

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • IP

Unknown

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab157203 & HEK-293 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Predicted band size: 57 kDa

false

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • WB

Lab

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

Western blot : Anti-CDK5RAP3 antibody [EPR11294(B)] ab157203 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 62 kDa in Wild-type A549 cell lysates with no signal observed at this size in CDK5RAP3 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/2000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CDK5RAP3 knockout A549 at 20 µg

Lane 3:

Calu-3 at 20 µg

Lane 4:

SK-N-SH at 20 µg

Lane 5:

SK-OV-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 62 kDa,37 kDa

true

Exposure time: 60s

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • WB

Lab

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

All lanes:

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • WB

Lab

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

All lanes:

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution

Lane 1:

CTLL-2 (Mouse T lymphocyte) whole cell lysates at 20 µg

Lane 2:

EL4 (Mouse lymphoma T lymphocyte) whole cell lysates at 20 µg

Lane 3:

H9 (Human lymphoma T lymphocyte) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)
  • WB

Unknown

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (AB157203)

All lanes:

Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Lane 3:

A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Predicted band size: 57 kDa

false

  • Carrier free

    Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11294(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate adapter of E3 ligase complexes mediating ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, and which is involved in various processes, such as ribosome recycling and reticulophagy (also called ER-phagy) (PubMed : 23152784, PubMed : 30635284, PubMed : 32851973, PubMed : 36121123, PubMed : 36543799, PubMed : 37595036, PubMed : 38383785, PubMed : 38383789). As part of the UREL complex, plays a key role in ribosome recycling by promoting mono-ufmylation of RPL26/uL24 subunit of the 60S ribosome (PubMed : 38383785, PubMed : 38383789). Ufmylation of RPL26/uL24 occurs on free 60S ribosomes following ribosome dissociation : it weakens the junction between post-termination 60S subunits and SEC61 translocons, promoting release and recycling of the large ribosomal subunit from the endoplasmic reticulum membrane (PubMed : 38383785, PubMed : 38383789). Ufmylation of RPL26/uL24 and subsequent 60S ribosome recycling either take place after normal termination of translation or after ribosome stalling during cotranslational translocation at the endoplasmic reticulum (PubMed : 32851973, PubMed : 37595036, PubMed : 38383785, PubMed : 38383789). Within the UREL complex, CDK5RAP3 acts as a substrate adapter that constrains UFL1 ligase activity to mono-ufmylate RPL26/uL24 at 'Lys-134' (PubMed : 36121123, PubMed : 38383785, PubMed : 38383789). The UREL complex is also involved in reticulophagy in response to endoplasmic reticulum stress by promoting ufmylation of proteins such as CYB5R3, thereby promoting lysosomal degradation of ufmylated proteins (PubMed : 36543799). Also acts as a regulator of transcription : negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed : 17785205, PubMed : 20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed : 15790566, PubMed : 19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed : 16173922). May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed : 23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed : 21283629). Required for liver development (By similarity).. (Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.
See full target information CDK5RAP3
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:146 PubMed40188151

2025

Neuronal CDK5RAP3 deficiency leads to encephalo-dysplasia via upregulation of N-glycosylases and glycogen deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Fanghui Chen,Minghui Xiang,Zhipeng Wang,Fan Yang,Junzhi Zhou,Zihan Deng,Susu Wang,Ping Li,Jieqi Tew,Wei Zhang,Honglin Li,Yong Teng,Xiaobin Zhu,Yafei Cai

International journal of biological sciences 20:2440-2453 PubMed38725860

2024

TSPAN6 reinforces the malignant progression of glioblastoma via interacting with CDK5RAP3 and regulating STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Fei-Hua Du,Rou-Xin Wang,Wen-Bo Han,Xing Lv,Ling-Hui Zeng,Guo-Qing Chen

Cancer gene therapy 30:22-37 PubMed35999359

2022

CDK5RAP3 acts as a tumour suppressor in gastric cancer through the infiltration and polarization of tumour-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Bin Wang,You-Xin Gao,Yin-Hua Ye,Tong-Xing Lin,Ping Li,Jian-Xian Lin,Qi-Yue Chen,Long-Long Cao,Mi Lin,Ru-Hong Tu,Ju-Li Lin,Ze-Ning Huang,Hua-Long Zheng,Jian-Wei Xie,Chao-Hui Zheng,Chang-Ming Huang

Journal of Cancer 11:3172-3179 PubMed32231721

2020

TBX2 Expression predicts Tumor Recurrence and Adjuvant Chemotherapy Benefits in Gastric Cancer Patients following R0 Resection: a proposed approach for risk stratification.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Lu,Yu Xu,Yao-Hui Wang,Xiao-Yan Huang,Yuan Wu,Jian-Wei Xie,Jia-Bin Wang,Jian-Xian Lin,Ping Li,Chao-Hui Zheng,Ai-Min Huang,Chang-Ming Huang

Bioscience reports 40: PubMed31829413

2019

Ubiquitin fold modifier 1 activates NF-κB pathway by down-regulating LZAP expression in the macrophage of diabetic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolei Hu,Hengyan Zhang,Yuan Song,Langen Zhuang,Qingqing Yang,Minglin Pan,Fengling Chen

Cancer cell international 19:282 PubMed31728130

2019

CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Xian Lin,Xiong-Feng Weng,Xin-Sheng Xie,Ning-Zi Lian,Sheng-Liang Qiu,Jia-Bin Wang,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Ru-Hong Tu,Ying-Hong Yang,Si-Jia Liu,Min Hu,Yi-Ke Lin,Chang-Ming Huang,Chao-Hui Zheng,Ping Li,Jian-Wei Xie

Journal of Cancer 10:4488-4498 PubMed31528213

2019

CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Yue Chen,Li-Chao Liu,Jia-Bin Wang,Jian-Wei Xie,Jian-Xian Lin,Jun Lu,Long-Long Cao,Mi Lin,Ru-Hong Tu,Chang-Ming Huang,Ping Li,Chao-Hui Zheng

Development (Cambridge, England) 146: PubMed30635284

2019

CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Yang,Huanmin Wang,Boxi Kang,Bin Chen,Yaoyao Shi,Shuchun Yang,Lihong Sun,Yufang Liu,Weidi Xiao,Tao Zhang,Juntao Yang,Ye Zhang,Mingzhao Zhu,Ping Xu,Yongsheng Chang,Yuyan Jia,Yue Huang

World journal of gastroenterology 24:3898-3907 PubMed30228783

2018

Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Xian Lin,Xin-Sheng Xie,Xiong-Feng Weng,Chao-Hui Zheng,Jian-Wei Xie,Jia-Bin Wang,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Ru-Hong Tu,Ping Li,Chang-Ming Huang

Journal of experimental & clinical cancer research 37:59 PubMed29540196

2018

CDK5RAP3 suppresses Wnt/β-catenin signaling by inhibiting AKT phosphorylation in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Hui Zheng,Jia-Bin Wang,Man-Qiang Lin,Peng-Yang Zhang,Li-Chao Liu,Jian-Xian Lin,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Ru-Hong Tu,Jian-Wei Xie,Ping Li,Chang-Ming Huang
View all publications
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Product promise

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