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AB241554

Anti-Cdk6 antibody [98D]

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(6 Publications)

Mouse Recombinant Monoclonal CDK6 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

View Alternative Names

CDKN6, CDK6, Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE

3 Images
Western blot - Anti-Cdk6 antibody [98D] (AB241554)
  • WB

Supplier Data

Western blot - Anti-Cdk6 antibody [98D] (AB241554)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Negative control : PANC-1, Saos-2. (PMID : 8114739).

All lanes:

Western blot - Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/mL

Lane 1:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 2:

K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 3:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

Saos-2 (human osteosarcoma epithelial), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Western blot - Anti-Cdk6 antibody [98D] (AB241554)
  • WB

Lab

Western blot - Anti-Cdk6 antibody [98D] (AB241554)

Lanes 1 - 2 : Merged signal (red and green). Green - ab241554 observed at 38 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab241554 was shown to react with Cdk6 in HeLa wild-type cells in Western blot with loss of signal observed in CDK6 knockout cell line ab266059 (CDK6 knockout cell lysate ab257088). Wild-type HeLa and CDK6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab241554 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Cdk6 antibody [98D] (ab241554) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CDK6 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CDK6 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cdk6-knockout-hela-cell-line-ab266059'>ab266059</a>)

Predicted band size: 37 kDa

Observed band size: 38 kDa

false

Western blot - Anti-Cdk6 antibody [98D] (AB241554)
  • WB

Supplier Data

Western blot - Anti-Cdk6 antibody [98D] (AB241554)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/mL

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

  • Carrier free

    Anti-Cdk6 antibody [98D] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

98D

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.518 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.518 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.518 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdk6 also known as Cyclin-dependent kinase 6 is a protein kinase involved in the regulation of the cell cycle. It has a molecular weight of approximately 40 kDa. Cdk6 is expressed in various tissues including lymphoid tissues and the brain. It plays a mechanical role by phosphorylating target proteins which impacts cell cycle progression particularly during the transition from G1 phase to S phase. Cdk6 can work in conjunction with D-type cyclins to form a complex that is critical for its activity.
Biological function summary

Cdk6 functions as a regulator of the cell cycle. It participates in a complex with cyclin D to control the G1 phase progression. This activity is essential for the regulation of cell division and proliferation. The association with cyclin D allows Cdk6 to phosphorylate the retinoblastoma protein (Rb) leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis.

Pathways

Cdk6 is an important component of the cell cycle control pathway. It interacts primarily with the retinoblastoma protein and Cyclin D1 in this context. The cell cycle pathway is critical for controlled cell proliferation and its dysregulation can lead to diseases like cancer. Another involved pathway is the PI3K/AKT pathway which can activate Cdk6 activity through upstream signaling events implicating Cdk6 in cellular responses to growth signals.

Cdk6 has a significant role in cancer development due to its involvement in uncontrolled cell proliferation. Its overexpression or dysregulation is often linked to cancers such as leukemia and glioblastoma. In cancer the interaction with proteins like cyclin D1 can lead to unchecked progression through the cell cycle contributing to oncogenesis. Additionally Cdk6 inhibitors are being explored as therapeutic agents in cancer treatment due to their potential to restore control over cell cycle progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and negatively regulates cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed : 23918663).
See full target information CDK6
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 30:456 PubMed40762017

2025

Acute myeloid leukemia with plasmacytoid dendritic cell proliferation: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Pei,Yi Zhang,Han Xu,Pei Pei,Zhengyang Zhang,Hongxia Wang,Bei Zhang,Junjun Bai,Yingxin Zhao,Jingjing Gu,Zhiyu Fang,Miaomiao Liu,Qinglin Song

Communications biology 6:1041 PubMed37833461

2023

DNA damage induced by CDK4 and CDK6 blockade triggers anti-tumor immune responses through cGAS-STING pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Fan,Wancheng Liu,Yanqiong Zeng,Ying Zhou,Meiling Gao,Liping Yang,Hao Liu,Yueyue Shi,Lili Li,Jiayuan Ma,Jiayin Ruan,Ruyun Cao,Xiaoxia Jin,Jian Chen,Genhong Cheng,Heng Yang

Frontiers in genetics 12:694571 PubMed34616424

2021

Upregulation of Linc00284 Promotes Lung Cancer Progression by Regulating the miR-205-3p/c-Met Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Sheng,Weixi Guo,Fang Lu,Hongming Liu,Rongmu Xia,Feng Dong

Frontiers in genetics 12:673229 PubMed34322152

2021

Hsa_circ_0002062 Promotes the Proliferation of Pulmonary Artery Smooth Muscle Cells by Regulating the Hsa-miR-942-5p/CDK6 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yali Wang,Xiaoming Tan,Yunjiang Wu,Sipei Cao,Yueyan Lou,Liyan Zhang,Feng Hu

Journal of hepatocellular carcinoma 8:211-222 PubMed33854986

2021

High Expression of PIGC Predicts Unfavorable Survival in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xufeng Guo,Shan Tian,Pan Cao,Yishan Xie,Weiguo Dong

Drug design, development and therapy 14:1523-1534 PubMed32368011

2020

Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingbin Wu,Xiaochen Yuan,Bingwei Li,Ruiqin Han,Honggang Zhang,Ruijuan Xiu
View all publications
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Product promise

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