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Mouse Recombinant Monoclonal CDK6 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

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Images

Western blot - Anti-Cdk6 antibody [98D] (AB241554), expandable thumbnail
  • Western blot - Anti-Cdk6 antibody [98D] (AB241554), expandable thumbnail
  • Western blot - Anti-Cdk6 antibody [98D] (AB241554), expandable thumbnail

Publications

  • Communications biology 6:10412023
    DNA damage induced by CDK4 and CDK6 blockade triggers anti-tumor immune responses through cGAS-STING pathway.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Huimin Fan,Wancheng Liu,Yanqiong Zeng,Ying Zhou,Meiling Gao,Liping Yang,Hao Liu,Yueyue Shi,Lili Li,Jiayuan Ma,Jiayin Ruan,Ruyun Cao,Xiaoxia Jin,Jian Chen,Genhong Cheng,Heng Yang
    PubMed 37833461
  • Frontiers in genetics 12:6945712021
    Upregulation of Linc00284 Promotes Lung Cancer Progression by Regulating the miR-205-3p/c-Met Axis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Wang Sheng,Weixi Guo,Fang Lu,Hongming Liu,Rongmu Xia,Feng Dong
    PubMed 34616424

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Not recommended
Mouse
Tested
Not recommended
Rat
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

0.518 µg/mL

Notes

-

Species

Rat

Dilution info

0.518 µg/mL

Notes

-

Species

Human

Dilution info

0.518 µg/mL

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).

      Alternative names

      Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6

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      Mouse Recombinant Monoclonal CDK6 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

      Alternative names

      Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6

      Key facts

      Isotype

      IgG1

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      98D

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

      Supplementary info

      Activity summary

      Cdk6 also known as Cyclin-dependent kinase 6 is a protein kinase involved in the regulation of the cell cycle. It has a molecular weight of approximately 40 kDa. Cdk6 is expressed in various tissues including lymphoid tissues and the brain. It plays a mechanical role by phosphorylating target proteins which impacts cell cycle progression particularly during the transition from G1 phase to S phase. Cdk6 can work in conjunction with D-type cyclins to form a complex that is critical for its activity.

      Biological function summary

      Cdk6 functions as a regulator of the cell cycle. It participates in a complex with cyclin D to control the G1 phase progression. This activity is essential for the regulation of cell division and proliferation. The association with cyclin D allows Cdk6 to phosphorylate the retinoblastoma protein (Rb) leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis.

      Pathways

      Cdk6 is an important component of the cell cycle control pathway. It interacts primarily with the retinoblastoma protein and Cyclin D1 in this context. The cell cycle pathway is critical for controlled cell proliferation and its dysregulation can lead to diseases like cancer. Another involved pathway is the PI3K/AKT pathway which can activate Cdk6 activity through upstream signaling events implicating Cdk6 in cellular responses to growth signals.

      Associated diseases and disorders

      Cdk6 has a significant role in cancer development due to its involvement in uncontrolled cell proliferation. Its overexpression or dysregulation is often linked to cancers such as leukemia and glioblastoma. In cancer the interaction with proteins like cyclin D1 can lead to unchecked progression through the cell cycle contributing to oncogenesis. Additionally Cdk6 inhibitors are being explored as therapeutic agents in cancer treatment due to their potential to restore control over cell cycle progression.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Western blot - Anti-Cdk6 antibody [98D] (ab241554), expandable thumbnail

        Western blot - Anti-Cdk6 antibody [98D] (ab241554)

        Lanes 1 - 2: Merged signal (red and green). Green - ab241554 observed at 38 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

        ab241554 was shown to react with Cdk6 in HeLa wild-type cells in Western blot with loss of signal observed in CDK6 knockout cell line Human CDK6 knockout HeLa cell line ab266059 (CDK6 knockout cell lysate Human CDK6 knockout HeLa cell lysate ab257088). Wild-type HeLa and CDK6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab241554 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

        All lanes: Western blot - Anti-Cdk6 antibody [98D] (ab241554) at 1/2000 dilution

        Lane 1: Wild-type HeLa cell lysate at 20 µg

        Lane 2: CDK6 knockout HeLa cell lysate at 20 µg

        Performed under reducing conditions.

        Predicted band size: 37 kDa

        Observed band size: 38 kDa

      • Western blot - Anti-Cdk6 antibody [98D] (ab241554), expandable thumbnail

        Western blot - Anti-Cdk6 antibody [98D] (ab241554)

        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure time: 3 minutes.

        Negative control: PANC-1, Saos-2. (PMID: 8114739).

        All lanes: Western blot - Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/mL

        Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

        Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

        Lane 3: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate at 20 µg

        Lane 4: Saos-2 (human osteosarcoma epithelial), whole cell lysate at 20 µg

        Secondary

        All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

        Predicted band size: 37 kDa

        Observed band size: 37 kDa

      • Western blot - Anti-Cdk6 antibody [98D] (ab241554), expandable thumbnail

        Western blot - Anti-Cdk6 antibody [98D] (ab241554)

        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure time: 3 minutes.

        All lanes: Western blot - Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/mL

        Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

        Lane 2: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

        Secondary

        All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

        Predicted band size: 37 kDa

        Observed band size: 37 kDa

      Downloads

      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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