Anti-Cdk6 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 antibody (AB151247)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue stained for CDK6 protein at cytoplasm and nucleus with ab151247 at a 1/500 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody (AB151247)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling CDK6 protein at nucleus using ab151247 at a 1/500 dilution (Green). Alpha Tubulin, Cytoskeleton Marker was counterstained in red.

• WB

Supplier Data

Western blot - Anti-Cdk6 antibody (AB151247)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cdk6 antibody (ab151247) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-Cdk6 antibody (AB151247)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cdk6 antibody (ab151247) at 1/500 dilution

All lanes:

Raw264.7 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-Cdk6 antibody (AB151247)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cdk6 antibody (ab151247) at 1/1000 dilution

All lanes:

Rat2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-Cdk6 antibody (AB151247)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cdk6 antibody (ab151247) at 1/500 dilution

All lanes:

C6 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 39 kDa

false

• WB

CiteAb

Western blot - Anti-Cdk6 antibody (AB151247)

Cdk6 western blot using anti-Cdk6 antibody ab151247. Publication image and figure legend from Ma, L., Zhou, Y., et al., 2017, Oncotarget, PubMed 27911852.

ab151247 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab151247 please see the product overview.

XIST inhibition decreased HGC-27 cell proliferation and invasion(A) XIST expression in HGC-27 cells transduced with control shRNA vector (sh-ctrl) or XIST shRNA vector (sh-XIST). (B) The MTT assay revealed that XIST down-regulation significantly decreased cell proliferation. (C) Colony formation assay demonstrated that oncogenic survival was significantly decreased in sh-XIST cells compared with sh-ctrl cells. (D) sh-XIST cells displayed a significantly higher frequency of cells at the G1 phase and a lower frequency of cells at S phase. (E) The number of cells incorporating EdU was significantly decreased in the sh-XIST group when compared with the sh-ctrl group. (F) XIST down-regulation affected the expression of G1/S phase checkpoint proteins. (G) XIST down-regulation increased the proportion of apoptosis in HGC-27 cells. (H) The alteration of pro-apoptotic proteins (Bax and Bim) and anti-apoptotic proteins (Bcl-2 and Mcl-1) in sh-XIST cells. (I) XIST down-regulation decreased the HGC-27 cell invasion ability, as revealed by the boyden assay. (J) The expression levels of MMP-2, MMP-3, MMP-9, N-cadherin and Vimentin protein were lower in sh-XIST cells. The expression level of E-cadherin was higher.

false