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Knockout Tested Rabbit Recombinant Monoclonal CDK6 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 107 publications.


Images

Western blot - Anti-Cdk6 antibody [EPR4515] (AB124821), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (AB124821), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 antibody [EPR4515] (AB124821), expandable thumbnail
  • Western blot - Anti-Cdk6 antibody [EPR4515] (AB124821), expandable thumbnail
  • Western blot - Anti-Cdk6 antibody [EPR4515] (AB124821), expandable thumbnail

Publications

  • Nature communications 14:66992023
    Broad-spectrum kinome profiling identifies CDK6 upregulation as a driver of lenvatinib resistance in hepatocellular carcinoma.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Carmen Oi Ning Leung,Yang Yang,Rainbow Wing Hei Leung,Karl Kam Hei So,Hai Jun Guo,Martina Mang Leng Lei,Gregory Kenneth Muliawan,Yuan Gao,Qian Qian Yu,Jing Ping Yun,Stephanie Ma,Qian Zhao,Terence Kin Wah Lee
    PubMed 37872167
  • Clinical, cosmetic and investigational dermatology 16:2119-21282023
    LncRNA FENDRR Suppresses Melanoma Growth via Influencing c-Myc mRNA Level.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Changying Niu,Shenxing Tan
    PubMed 37581008

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50000 - 1/200000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

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    5 products for Alternative Version

      Target data

      Function

      Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).

      Alternative names

      Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6

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      Knockout Tested Rabbit Recombinant Monoclonal CDK6 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 107 publications.

      Alternative names

      Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR4515

      Purification technique

      Affinity purification Protein A

      Specificity

      Based on the immunogen sequence, we do not expect the antibody to cross-react with other CDK family members. No cross-reactivity testing has been performed.

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Stable for 12 months at -20°C

      Notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      Cdk6 also known as Cyclin-dependent kinase 6 is a protein kinase involved in the regulation of the cell cycle. It has a molecular weight of approximately 40 kDa. Cdk6 is expressed in various tissues including lymphoid tissues and the brain. It plays a mechanical role by phosphorylating target proteins which impacts cell cycle progression particularly during the transition from G1 phase to S phase. Cdk6 can work in conjunction with D-type cyclins to form a complex that is critical for its activity.

      Biological function summary

      Cdk6 functions as a regulator of the cell cycle. It participates in a complex with cyclin D to control the G1 phase progression. This activity is essential for the regulation of cell division and proliferation. The association with cyclin D allows Cdk6 to phosphorylate the retinoblastoma protein (Rb) leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis.

      Pathways

      Cdk6 is an important component of the cell cycle control pathway. It interacts primarily with the retinoblastoma protein and Cyclin D1 in this context. The cell cycle pathway is critical for controlled cell proliferation and its dysregulation can lead to diseases like cancer. Another involved pathway is the PI3K/AKT pathway which can activate Cdk6 activity through upstream signaling events implicating Cdk6 in cellular responses to growth signals.

      Associated diseases and disorders

      Cdk6 has a significant role in cancer development due to its involvement in uncontrolled cell proliferation. Its overexpression or dysregulation is often linked to cancers such as leukemia and glioblastoma. In cancer the interaction with proteins like cyclin D1 can lead to unchecked progression through the cell cycle contributing to oncogenesis. Additionally Cdk6 inhibitors are being explored as therapeutic agents in cancer treatment due to their potential to restore control over cell cycle progression.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      8 product images

      • Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821)

        Lanes 1- 2: Merged signal (red and green). Green - ab124821 observed at 37 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.

        ab124821 was shown to react with Cdk6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human CDK6 knockout HeLa cell line ab266059 (knockout cell lysate Human CDK6 knockout HeLa cell lysate ab257088) was used. Wild-type HeLa and Cdk6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124821 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/1000 dilution

        Lane 1: Wild-type HeLa cell lysate at 20 µg

        Lane 2: CDK6 knockout HeLa cell lysate at 20 µg

        Performed under reducing conditions.

        Predicted band size: 37 kDa

        Observed band size: 37 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (ab124821)

        ab124821 staining Cdk6 in wild-type HAP1 cells (top panel) and Cdk6 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124821 at 1/500 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 antibody [EPR4515] (ab124821)

        ab124821 at 1/100 dilution, staining Cdk6 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.

        Heat mediated antigen retrieval was performed with 0.01M citrate buffer, pH 6.0.

      • Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821)

        Lanes 1 and 2: Green signal from target - ab124821 observed at 37 kDa
        Lanes 3 and 4: Red signal from loading control - Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 observed at 42 kDa
        Lanes 5 and 6: Merged (red and green) signal
        ab124821 was shown to specifically react with CDK6 when CDK6 knockout samples were used. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab124821 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta actin) were diluted at 1/10 000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

        Lanes 1 - 2: Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/10000 dilution

        Lanes 3 - 4: Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/1000 dilution

        Lanes 5 - 6: Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821)

        Lanes 1, 3 and 5: Wild-type HAP1 cell lysate at 20 µg

        Lanes 2, 4 and 6: CDK6 knockout HAP1 cell lysate at 20 µg

        Predicted band size: 37 kDa

      • Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821)

        All lanes: Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/1000 dilution

        Lane 1: Jurkat cell lysate at 10 µg

        Lane 2: K562 cell lysate at 10 µg

        Lane 3: HeLa cell lysate at 10 µg

        Lane 4: 293T cell lysate at 10 µg

        Secondary

        All lanes: Goat-anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 37 kDa

        Observed band size: 37 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (ab124821)

        ICC/IF image of ab124821 at 1/100 dilution, staining Cdk6 in HeLa cells.

      • Flow Cytometry (Intracellular) - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-Cdk6 antibody [EPR4515] (ab124821)

        Overlay histogram showing HeLa cells stained with ab124821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124821, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      • Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821), expandable thumbnail

        Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821)

        Western blot: Anti-CDK6 antibody [EPR4515] (ab124821) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab124821 was shown to bind specifically to CDK6. A band was observed at 37 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in CDK6 knockout cell line. To generate this image, wild-type and CDK6 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

        All lanes: Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/1000 dilution

        Lane 1: Wild-type HCT 116 cell lysate at 20 µg

        Lane 2: CDK6 knockout HCT 116 cell lysate at 20 µg

        Lane 3: Wild-type HeLa ab255929 cell lysate at 20 µg

        Lane 4: CDK6 knockout HeLa ab260923 cell lysate at 20 µg

        Secondary

        All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

        Performed under reducing conditions.

        Predicted band size: 37 kDa

        Observed band size: 37 kDa

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