Rabbit Polyclonal CDK6 phospho Y24 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications.
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- Oncology letters 22:6282021Aqueous extracts of induce S-phase arrest and apoptosis in human melanoma A375 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaihen Yu,Shi Zhong,Yuqing Sun,Haiyan Sun,Weiguo Chen,Yougui Li,Jianxun Zhu,Longxi Lu,Jinxi HuoPubMed 34267820
- Molecular medicine reports 23:2021Knockdown of lncRNA DLEU1 inhibits the tumorigenesis of oral squamous cell carcinoma via regulation of miR‑149‑5p/CDK6 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianzhu Lv,Hongjing Liu,Yadong Wu,Wentao HuangPubMed 33880596
- American journal of cancer research 10:3267-32842020Targeting the polycomb repressive complex-2 related proteins with novel combinational strategies for nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunyu Zhu et. al.PubMed 33163269
- Cellular oncology (Dordrecht) :2020[10]-Gingerol improves doxorubicin anticancer activity and decreases its side effects in triple negative breast cancer models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Carolina Baptista Moreno Martin et. al.PubMed 32761561
- eLife 9:2020p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Che et. al.PubMed 32125276
- Artificial cells, nanomedicine, and biotechnology 47:2980-29882019Bilobalide protects H9c2 cell from oxygen-glucose-deprivation-caused damage through upregulation of miR-27a.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAilin Cao,Xiangting LiPubMed 31322008
- Pathology oncology research : POR 26:1301-13112019Upregulation of RUNX1 Suppresses Proliferation and Migration through Repressing VEGFA Expression in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Liu et. al.PubMed 31289995
- Thoracic cancer 9:939-9492018Long non-coding RNA OIP5-AS1 promotes proliferation of lung cancer cells and leads to poor prognosis by targeting miR-378a-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaolong Wang et. al.PubMed 29897167
- Cell cycle (Georgetown, Tex.) 14:3734-472015SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status.Applications:WBReactive species:Unspecified reactive speciesM Gordian Adam,Sonja Matt,Sven Christian,Holger Hess-Stumpp,Andrea Haegebarth,Thomas G Hofmann,Carolyn AlgirePubMed 26654769
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).
Alternative names
Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDKN6, CDK6
Recommended products
Rabbit Polyclonal CDK6 phospho Y24 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications.
Alternative names
Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDKN6, CDK6
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab131469 was purified by affinity-chromatography using epitope-specific phospho peptide. The antibody against non-phospho peptide was removed by chromatography using non-phospho peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cdk6 also known as Cyclin-dependent kinase 6 is a protein kinase involved in the regulation of the cell cycle. It has a molecular weight of approximately 40 kDa. Cdk6 is expressed in various tissues including lymphoid tissues and the brain. It plays a mechanical role by phosphorylating target proteins which impacts cell cycle progression particularly during the transition from G1 phase to S phase. Cdk6 can work in conjunction with D-type cyclins to form a complex that is critical for its activity.
- Biological function summary
Cdk6 functions as a regulator of the cell cycle. It participates in a complex with cyclin D to control the G1 phase progression. This activity is essential for the regulation of cell division and proliferation. The association with cyclin D allows Cdk6 to phosphorylate the retinoblastoma protein (Rb) leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis.
- Pathways
Cdk6 is an important component of the cell cycle control pathway. It interacts primarily with the retinoblastoma protein and Cyclin D1 in this context. The cell cycle pathway is critical for controlled cell proliferation and its dysregulation can lead to diseases like cancer. Another involved pathway is the PI3K/AKT pathway which can activate Cdk6 activity through upstream signaling events implicating Cdk6 in cellular responses to growth signals.
- Associated diseases and disorders
Cdk6 has a significant role in cancer development due to its involvement in uncontrolled cell proliferation. Its overexpression or dysregulation is often linked to cancers such as leukemia and glioblastoma. In cancer the interaction with proteins like cyclin D1 can lead to unchecked progression through the cell cycle contributing to oncogenesis. Additionally Cdk6 inhibitors are being explored as therapeutic agents in cancer treatment due to their potential to restore control over cell cycle progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Cdk6 (phospho Y24) antibody (ab131469)
All lanes: Western blot - Anti-Cdk6 (phospho Y24) antibody (ab131469) at 1/500 dilution
Lane 1: Extract from 293 cells; untreated
Lane 2: Extract from 293 cells; treated with starvation
Predicted band size: 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 (phospho Y24) antibody (ab131469)
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling Cdk6 (phospho Y24) with ab131469 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 (phospho Y24) antibody (ab131469)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling Cdk6 (phospho Y24) with ab131469 at 1/50 dilution. The image on the right is preincubated with blocking peptide.
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