Rabbit Polyclonal CDK7 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDK7 aa 200 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CDK7 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P50613
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Associated Products
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Target data
Function
Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts (PubMed:9852112). Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.
Alternative names
CAK, CAK1, CDKN7, MO15, STK1, CDK7, Cyclin-dependent kinase 7, 39 kDa protein kinase, CDK-activating kinase 1, Cell division protein kinase 7, Serine/threonine-protein kinase 1, TFIIH basal transcription factor complex kinase subunit, p39 Mo15
Recommended products
Rabbit Polyclonal CDK7 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDK7 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CDK7 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P50613
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cyclin-dependent kinase 7 (Cdk7) also known as CAK is a protein that functions as a serine/threonine kinase. It has a mass of approximately 37 kDa and is expressed in various tissues including the liver heart and testis. Cdk7 forms part of the CDK-activating complex required for the cell cycle's progression and transcription processes. By phosphorylating important sites on target proteins Cdk7 contributes to the regulation of the cell cycle and RNA polymerase II-mediated transcription.
- Biological function summary
Cdk7 interacts with other molecules playing a fundamental role in the regulation of cell division and transcription. Cdk7 is a component of the Cdk-activating kinase (CAK) complex alongside cyclin H and MAT1. This complex enables activation of other cyclin-dependent kinases through phosphorylation which is essential for DNA replication and repair. As part of the transcription complex TFIIH Cdk7 enhances RNA polymerase II's transcriptional activity influencing gene expression and cellular responses to external signals.
- Pathways
Cdk7 participates in significant regulatory pathways controlling cell cycle and transcription. It is integral to the p53 Signaling and Cell Cycle pathways where it facilitates the activation of CDK1 CDK2 and CDK4/6 key players in cell cycle progression from the G1 to S phase. Through these pathways Cdk7 maintains cellular control upon environmental stress responses and DNA damage providing connections to tumor suppressor proteins such as p53.
- Associated diseases and disorders
Cdk7 has associations with cancer and neurodegenerative diseases. Its dysregulation can lead to unchecked cell proliferation contributing to the development of various cancers like breast and lung cancer. Cdk7's role in transcription implicates it in disorders like Alzheimer's disease where altered transcriptional regulation is observed. Furthermore its interaction with proteins such as p53 links it to cancer as p53 mutations can result in the loss of control over the cell cycle exacerbating tumorigenic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody (ab262854)
Paraffin-embedded human testis tissue stained for Cdk7 using ab262854 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Cdk7 antibody (ab262854)
All lanes: Western blot - Anti-Cdk7 antibody (ab262854) at 0.4 µg/mL
Lane 1: Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody (ab262854)
Paraffin-embedded human cerebellum tissue stained for Cdk7 using ab262854 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody (ab262854)
Paraffin-embedded human placenta tissue stained for Cdk7 using ab262854 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody (ab262854)
Paraffin-embedded human liver tissue stained for Cdk7 using ab262854 at 1/500 dilution in immunohistochemical analysis.
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