AB115155

Anti-Cdk8 antibody

Name: Anti-Cdk8 antibody (ab115155) | Abcam
Brand: Abcam Limited
SKU: AB115155
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal CDK8 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CDK8 aa 350 to C-terminus.

View Alternative Names

Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35, CDK8

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Immunoprecipitation - Anti-Cdk8 antibody (AB115155)

Immunoprecipitation of Cdk8 (3 μg of antibody per mg of HeLa cells lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdk8 antibody (ab115155); Lane 2-Immunoprecipitation with an anti-Cdk8 antibody that recognizes an upstream epitope; Lane 3- Immunoprecipitation with IgG control. An anti-Cdk8 antibody used in lane 2 was used at 0.4 μg/ml for western blot. Detection : Chemiluminescence with exposure time of 30 sec.

All lanes:

Immunoprecipitation - Anti-Cdk8 antibody (ab115155)

Predicted band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CDK8 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49336

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Medaka fish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Tilapia": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified using an epitope specific to CDK8 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdk8 also known as Cyclin-dependent kinase 8 forms an important component of the transcription regulation machinery. It has a molecular mass of about 66 kDa and is widely expressed in various tissues though its abundance can vary. Cdk8 operates largely in the nucleus of cells where it participates in regulating gene expression. Cdk8 acts as a kinase by modifying other proteins through phosphorylation which alters their activity and function.

Biological function summary

Cdk8 plays a critical role in modulating transcriptional responses. It does this by becoming part of the Mediator complex which is essential for transcription regulation by RNA Polymerase II. The Mediator complex where Cdk8 acts as a kinase influences the initiation and regulation of gene expression. Cdk8 phosphorylates several transcription factors and other components affecting their ability to activate or repress transcription.

Pathways

Cyclin-dependent kinase 8 functions prominently in the Wnt/β-catenin signaling and Notch signaling pathways both essential for development and cell differentiation. In the Wnt pathway it interacts closely with proteins like β-catenin to influence gene activation. In the Notch pathway it modulates transcription factors related to cellular differentiation processes. These interactions underline its role as a regulator in carefully managing cellular responses to signaling cues.

Cdk8 has been implicated in cancer and congenital disorders. Its overactivity can lead to uncontrolled cell growth contributing to tumor progression notably in colorectal cancer where it affects genes in the Wnt pathway. Beyond cancer its dysfunction links to congenital heart defects where it affects cardiogenesis-associated proteins such as NOTCH1. Cdk8’s involvement in these pathways highlights its part in both regular development and disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.

See full target information CDK8

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 20:305 PubMed33093914

2020

lncRNA UASR1 sponges miR-107 in colorectal cancer to upregulate oncogenic CDK8 and promote cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Qizhi Zhang,Zhaosheng Chen

mBio 10: PubMed30723126

2019

Didehydro-Cortistatin A Inhibits HIV-1 by Specifically Binding to the Unstructured Basic Region of Tat.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Mediouni,Krishna Chinthalapudi,Mary K Ekka,Ippei Usui,Joseph A Jablonski,Mark A Clementz,Guillaume Mousseau,Jason Nowak,Venkat R Macherla,Jacob N Beverage,Eduardo Esquenazi,Phil Baran,Ian Mitchelle S de Vera,Douglas Kojetin,Erwann P Loret,Kendall Nettles,Souvik Maiti,Tina Izard,Susana T Valente

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdk8 antibody

Immunoprecipitation - Anti-Cdk8 antibody (AB115155)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise