AB108290

Anti-CDKA1 / DOC1 antibody [Clone-R76]

RabMAb
Recombinant
KO Validated
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CDKA1 / DOC1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CDKAP1, DOC1, CDK2AP1, Cyclin-dependent kinase 2-associated protein 1, CDK2-associated protein 1, Deleted in oral cancer 1, Putative oral cancer suppressor, DOC-1

2 Images

Lab

Western blot - Anti-CDKA1 / DOC1 antibody [Clone-R76] (AB108290)

Western blot : Anti-CDKA1 / DOC1 antibody [Clone-R76] ab108290 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 14 kDa in Wild-type A549 cell lysates with no signal observed at this size in CDK2AP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CDKA1 / DOC1 antibody [Clone-R76] (ab108290) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CDK2AP1 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

Raji at 20 µg

Secondary

All lanes:

Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 14 kDa

false

Unknown

Western blot - Anti-CDKA1 / DOC1 antibody [Clone-R76] (AB108290)

All lanes:

Western blot - Anti-CDKA1 / DOC1 antibody [Clone-R76] (ab108290) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HUVEC cell lysate at 10 µg

Lane 4:

LNCaP cell lysate at 10 µg

Predicted band size: 12 kDa

false

Carrier free

Anti-CDKA1 / DOC1 antibody [Clone-R76] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Clone-R76

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of cyclin-dependent kinase CDK2 (By similarity). Also acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 20523938, PubMed : 28977666).

See full target information CDK2AP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 15:1929-1945 PubMed39113208

2024

Novel circular RNA hsa_circ_0036683 suppresses proliferation and migration by mediating the miR-4664-3p/CDK2AP2 axis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Han Zhang,Jiaxuan Xin,Shu-Yang Xie,Fei Jiao,You-Jie Li,Meng-Yuan Chu,Junming Qiu,Yun-Fei Yan

The Journal of biological chemistry 287:41103-17 PubMed23074223

2012

A novel regulatory factor recruits the nucleosome remodeling complex to wingless integrated (Wnt) signaling gene promoters in mouse embryonic stem cells.

Applications

Species

Mouse

Jeffrey J Kim,Omar Khalid,Sheynie Vo,Ho-hyun Sun,David T W Wong,Yong Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDKA1 / DOC1 antibody [Clone-R76]

Western blot - Anti-CDKA1 / DOC1 antibody [Clone-R76] (AB108290)

Western blot - Anti-CDKA1 / DOC1 antibody [Clone-R76] (AB108290)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise