AB169531

Anti-CDKAL1 antibody [EPR11621-26]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal CDKAL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Threonylcarbamoyladenosine tRNA methylthiotransferase, CDK5 regulatory subunit-associated protein 1-like 1, tRNA-t(6)A37 methylthiotransferase, CDKAL1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (AB169531)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (AB169531)

Immunofluorescent analysis of HeLa cells labeling CDKAL1 with ab169531 at 1/50 dilution. DAPI nuclear staining (blue).

Unknown

Western blot - Anti-CDKAL1 antibody [EPR11621-26] (AB169531)

All lanes:

Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

MDA-MB-435 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 65 kDa

false

Unknown

Western blot - Anti-CDKAL1 antibody [EPR11621-26] (AB169531)

All lanes:

Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from HepG2 cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 65 kDa

false

Carrier free

Anti-CDKAL1 antibody [EPR11621-26] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11621-26

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.

See full target information CDKAL1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 24:508 PubMed39103807

2024

Identification and validation of genes associated with prognosis of cisplatin-resistant ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dajiang Liu,Ruiyun Li,Yidan Wang,Dan Li,Leilei Li

Clinical and experimental pharmacology & physiology 50:307-315 PubMed36628934

2023

FTO demethylates m6A modifications in CDKAL1 mRNA and promotes gastric cancer chemoresistance by altering mitochondrial dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Na Liu,Chang Liu,Zixuan Wang,Longqing Wang,Jiang Wang,Jing Kong

Scientific reports 7:7326 PubMed28779110

2017

CDK5 Regulatory Subunit-Associated Protein 1-like 1 Negatively Regulates Adipocyte Differentiation through Activation of Wnt Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumi Take,Hironori Waki,Wei Sun,Takahito Wada,Jing Yu,Masahiro Nakamura,Tomohisa Aoyama,Toshimasa Yamauchi,Takashi Kadowaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDKAL1 antibody [EPR11621-26]

Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (AB169531)

Western blot - Anti-CDKAL1 antibody [EPR11621-26] (AB169531)

Western blot - Anti-CDKAL1 antibody [EPR11621-26] (AB169531)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (3)

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