Rabbit Polyclonal CDKL5 antibody. Suitable for WB and reacts with Mouse samples. Cited in 5 publications.
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Publications
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- Nature communications 13:6682022Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Clemm von Hohenberg,Sandra Müller,Sibylle Schleich,Matthias Meister,Jonathan Bohlen,Thomas G Hofmann,Aurelio A TelemanPubMed 35115540
- Nature communications 11:19242020A kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Young Kim et. al.PubMed 32317630
- The EMBO journal 37:2018Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucas L Baltussen et. al.PubMed 30266824
- Proceedings of the National Academy of Sciences of 109:21516-212012Loss of CDKL5 disrupts kinome profile and event-related potentials leading to autistic-like phenotypes in mice.
- Clinical genetics 76:357-712009Mutational spectrum of CDKL5 in early-onset encephalopathies: a study of a large collection of French patients and review of the literature.Applications:WB, ICC/IFReactive species:HumanC Nemos,L Lambert,F Giuliano,B Doray,A Roubertie,A Goldenberg,B Delobel,V Layet,M A N'guyen,A Saunier,F Verneau,P Jonveaux,C PhilippePubMed 19793311
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-3.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Mediates phosphorylation of MECP2. May regulate ciliogenesis.
Alternative names
Cyclin-dependent kinase-like 5, Cdkl5
Recommended products
Rabbit Polyclonal CDKL5 antibody. Suitable for WB and reacts with Mouse samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Swissprot predicts mouse CDKL5 protein to be ~105 kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118 kDa on WB. On this basis we think that the ~120 kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120 kDa band is blocked by the immunising peptide. The larger ~200 kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomWestern blot - Anti-CDKL5 antibody (ab22453)
Swissprot predicts mouse CDKL5 protein to be ~105kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118kDa on WB. On this basis we think that the ~120kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120kDa band is blocked by the immunising peptide. The larger >150kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.
All lanes: Western blot - Anti-CDKL5 antibody (ab22453) at 3 µg/mL
Lane 1: mouse cortex at 20 µg
Lane 2: mouse cortex at 20 µg with Human CDKL5 peptide (ab25756)
Predicted band size: 116 kDa
Observed band size: 120 kDa, 200 kDa
Exposure time: 10min
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