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Rabbit Polyclonal CDKL5 antibody. Suitable for WB and reacts with Mouse samples. Cited in 5 publications.


Images

Western blot - Anti-CDKL5 antibody (AB22453), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Predicted
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

1.00000-3.00000 µg/mL

Notes

-

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Alternative names

Recommended products

Rabbit Polyclonal CDKL5 antibody. Suitable for WB and reacts with Mouse samples. Cited in 5 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

Swissprot predicts mouse CDKL5 protein to be ~105 kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118 kDa on WB. On this basis we think that the ~120 kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120 kDa band is blocked by the immunising peptide. The larger ~200 kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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1 product image

  • Western blot - Anti-CDKL5 antibody (ab22453), expandable thumbnail
    This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    Western blot - Anti-CDKL5 antibody (ab22453)

    Swissprot predicts mouse CDKL5 protein to be ~105kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118kDa on WB. On this basis we think that the ~120kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120kDa band is blocked by the immunising peptide. The larger >150kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.

    All lanes: Western blot - Anti-CDKL5 antibody (ab22453) at 3 µg/mL

    All lanes: mouse cortex at 20 µg

    Predicted band size: 116 kDa

    Observed band size: 120 kDa, 200 kDa

    Exposure time: 10min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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