Skip to main content

Rabbit Recombinant Monoclonal CDKL5 antibody. Suitable for WB, IP, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry (Intracellular) - Anti-CDKL5 antibody [EPR26906-28] (AB315220), expandable thumbnail
  • Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] (AB315220), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] (AB315220), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] (AB315220), expandable thumbnail
  • Western blot - Anti-CDKL5 antibody [EPR26906-28] (AB315220), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)ICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Expected
Expected
Tested
Not recommended
Rat
Tested
Expected
Expected
Expected
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Mediates phosphorylation of MECP2 (PubMed:15917271, PubMed:16935860). May regulate ciliogenesis (PubMed:29420175).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CDKL5 antibody. Suitable for WB, IP, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26906-28

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry (Intracellular) - Anti-CDKL5 antibody [EPR26906-28] (ab315220), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CDKL5 antibody [EPR26906-28] (ab315220)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CDKL5 with ab315220 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] (ab315220), expandable thumbnail

    Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] (ab315220)

    CDKL5 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab315220 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315220 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 2: ab315220 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315220 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] (ab315220) at 1/30 dilution

    All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 24s

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] (ab315220), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] (ab315220)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CDKL5 with ab315220 at 1/50 (10.12 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in Neuro-2a cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] (ab315220), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] (ab315220)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CDKL5 with ab315220 at 1/50 (10.12 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in HepG2 cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Western blot - Anti-CDKL5 antibody [EPR26906-28] (ab315220), expandable thumbnail

    Western blot - Anti-CDKL5 antibody [EPR26906-28] (ab315220)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ab315220 detects both two isoforms of CDKL5, separately named as CDKL5a and CDKL5b, around 130 and 100 kDa in Western blot (PMID: 20861382).

    All lanes: Western blot - Anti-CDKL5 antibody [EPR26906-28] (ab315220) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 50 µg

    Lane 2: Mouse lung tissue lysate at 50 µg

    Lane 3: Mouse liver tissue lysate at 50 µg

    Lane 4: Rat brain tissue lysate at 50 µg

    Lane 5: Rat lung tissue lysate at 50 µg

    Lane 6: Rat lilver tissue lysate at 50 µg

    Lane 7: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

    Lane 8: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 9: C2C12 (mouse myoblast) whole cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 100 kDa, 130 kDa, 110, 130 kDa

    Exposure time: 1s

  • Western blot - Anti-CDKL5 antibody [EPR26906-28] (ab315220), expandable thumbnail

    Western blot - Anti-CDKL5 antibody [EPR26906-28] (ab315220)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ab315220 detects both two isoforms of CDKL5, separately named as CDKL5a and CDKL5b, around 130 and 100 kDa in Western blot (PMID: 20861382).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CDKL5 antibody [EPR26906-28] (ab315220) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

    Lane 2: HepG2 transfected with siRNA specifically targeting CDKL5 whole cell lysate at 50 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 4: Human lung tissue lysate at 50 µg

    Lane 5: Human liver tissue lysate at 50 µg

    Lane 6: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 7: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 100 kDa, 130 kDa, 110, 130 kDa, 36 kDa

    Exposure time: 1s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com