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Rabbit Recombinant Monoclonal CDKL5 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (AB315221), expandable thumbnail
  • Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (AB315221), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (AB315221), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (AB315221), expandable thumbnail
  • Western blot - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (AB315221), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)ICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Expected
Expected
Tested
Not recommended
Rat
Tested
Expected
Expected
Expected
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Mediates phosphorylation of MECP2 (PubMed:15917271, PubMed:16935860). May regulate ciliogenesis (PubMed:29420175).

Alternative names

STK9, CDKL5, Cyclin-dependent kinase-like 5, Serine/threonine-protein kinase 9

Recommended products

Rabbit Recombinant Monoclonal CDKL5 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26906-28
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315221 is the carrier-free version of Anti-CDKL5 antibody [EPR26906-28] ab315220.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry (Intracellular) - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221)

    This data was developed using Anti-CDKL5 antibody [EPR26906-28] ab315220, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CDKL5 with Anti-CDKL5 antibody [EPR26906-28] ab315220 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221), expandable thumbnail

    Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221)

    This data was developed using Anti-CDKL5 antibody [EPR26906-28] ab315220, the same antibody clone in a different buffer formulation.

    CDKL5 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with Anti-CDKL5 antibody [EPR26906-28] ab315220 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CDKL5 antibody [EPR26906-28] ab315220 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-CDKL5 antibody [EPR26906-28] ab315220 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CDKL5 antibody [EPR26906-28] ab315220 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CDKL5 antibody [EPR26906-28] (Anti-CDKL5 antibody [EPR26906-28] ab315220) at 1/30 dilution

    All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 24s

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221)

    This data was developed using Anti-CDKL5 antibody [EPR26906-28] ab315220, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CDKL5 with Anti-CDKL5 antibody [EPR26906-28] ab315220 at 1/50 (10.12 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in Neuro-2a cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221)

    This data was developed using Anti-CDKL5 antibody [EPR26906-28] ab315220, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling CDKL5 with Anti-CDKL5 antibody [EPR26906-28] ab315220 at 1/50 (10.12 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in HepG2 cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Western blot - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221), expandable thumbnail

    Western blot - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221)

    This data was developed using Anti-CDKL5 antibody [EPR26906-28] ab315220, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-CDKL5 antibody [EPR26906-28] ab315220 detects both two isoforms of CDKL5, separately named as CDKL5a and CDKL5b, around 130 and 100 kDa in Western blot (PMID: 20861382).

    All lanes: Western blot - Anti-CDKL5 antibody [EPR26906-28] (Anti-CDKL5 antibody [EPR26906-28] ab315220) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 50 µg

    Lane 2: Mouse lung tissue lysate at 50 µg

    Lane 3: Mouse liver tissue lysate at 50 µg

    Lane 4: Rat brain tissue lysate at 50 µg

    Lane 5: Rat lung tissue lysate at 50 µg

    Lane 6: Rat lilver tissue lysate at 50 µg

    Lane 7: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

    Lane 8: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 9: C2C12 (mouse myoblast) whole cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 100 kDa, 130 kDa, 110, 130 kDa

    Exposure time: 1s

  • Western blot - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221), expandable thumbnail

    Western blot - Anti-CDKL5 antibody [EPR26906-28] - BSA and Azide free (ab315221)

    This data was developed using Anti-CDKL5 antibody [EPR26906-28] ab315220, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-CDKL5 antibody [EPR26906-28] ab315220 detects both two isoforms of CDKL5, separately named as CDKL5a and CDKL5b, around 130 and 100 kDa in Western blot (PMID: 20861382).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CDKL5 antibody [EPR26906-28] (Anti-CDKL5 antibody [EPR26906-28] ab315220) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

    Lane 2: HepG2 transfected with siRNA specifically targeting CDKL5 whole cell lysate at 50 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 4: Human lung tissue lysate at 50 µg

    Lane 5: Human liver tissue lysate at 50 µg

    Lane 6: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 7: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 100 kDa, 130 kDa, 110, 130 kDa, 36 kDa

    Exposure time: 1s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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