Skip to main content

Rabbit Recombinant Monoclonal p14ARF antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] (AB216602), expandable thumbnail
  • Western blot - Anti-CDKN2A/p14ARF antibody [SP271] (AB216602), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] (AB216602), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] (AB216602), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] (AB216602), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/400
Notes

(Incubate for 1 hour at room temperature).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400 - 1/420
Notes

(Incubate for 30 minutes at 4°C).

Tested
Tested

Species
Human
Dilution info
1/100
Notes

(Primary incubation for 10 minutes at room temperature).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Plays a role in inhibiting ribosome biogenesis, perhaps by binding to the nucleolar localization sequence of transcription termination factor TTF1, and thereby preventing nucleolar localization of TTF1 (By similarity). Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform smARF. May be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.

Alternative names

CDKN2, MLM, CDKN2A, Tumor suppressor ARF, Alternative reading frame, Cyclin-dependent kinase inhibitor 2A, p14ARF, ARF

Recommended products

Rabbit Recombinant Monoclonal p14ARF antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SP271
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602)

    "Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/500 (0.86 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control."

  • Western blot - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602), expandable thumbnail

    Western blot - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602)

    All lanes: Western blot - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602) at 1/400 dilution

    All lanes: HeLa cell lysate

    Predicted band size: 14 kDa

  • Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/420 dilution (1.02μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical squamous cell carcinoma labeling CDKN2A/p14ARF with ab216602 at 1/100 dilution.

  • Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] (ab216602)

    Intracellular flow cytometric analysis of HEK293 cells labeling CDKN2A/p14ARFwith ab216602 at 1/400 dilution (green) compared to negative control rabbit IgG (blue) .

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com