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AB271999

Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free

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Rabbit Recombinant Monoclonal p14ARF antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CDKN2, MLM, CDKN2A, Tumor suppressor ARF, Alternative reading frame, Cyclin-dependent kinase inhibitor 2A, p14ARF, ARF

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical squamous cell carcinoma labeling CDKN2A/p14ARF with ab216602 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).

Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)

Intracellular flow cytometric analysis of HEK293 cells labeling CDKN2A/p14ARF with ab216602 at 1/400 dilution (green) compared to negative control rabbit IgG (blue) .

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).

Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/420 dilution (1.02 μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (AB271999)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/500 (0.86 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).

  • Unconjugated

    Anti-CDKN2A/p14ARF antibody [SP271]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CDKN2A/p14ARF antibody [SP271]

  • 578 PE

    PE Anti-CDKN2A/p14ARF antibody [SP271]

  • 660 APC

    APC Anti-CDKN2A/p14ARF antibody [SP271]

  • HRP

    HRP Anti-CDKN2A/p14ARF antibody [SP271]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CDKN2A/p14ARF antibody [SP271]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CDKN2A/p14ARF antibody [SP271]

  • Alkaline Phosphatase

    Alkaline Phosphatase Anti-CDKN2A/p14ARF antibody [SP271]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CDKN2A/p14ARF antibody [SP271]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CDKN2A/p14ARF antibody [SP271]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CDKN2A/p14ARF antibody [SP271]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP271

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>(Incubate for 1 hour at room temperature).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>(Primary incubation for 10 minutes at room temperature).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>(Incubate for 30 minutes at 4°C).</p>" } } }
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Product details

ab271999 is the carrier-free version of ab216602.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Plays a role in inhibiting ribosome biogenesis, perhaps by binding to the nucleolar localization sequence of transcription termination factor TTF1, and thereby preventing nucleolar localization of TTF1 (By similarity). Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.. Isoform smARF. May be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.
See full target information CDKN2A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com