Anti-CDKN2A/p16INK4a antibody [2D9A12]

• IHC-P

AbReview12857****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

ab54210 staining CDKN2A/p16INK4a in human cervix and ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000) for 20 minutes at 25°C. An undiluted HRP-conjugated mouse polymer was used as the secondary antibody.

Image is courtesy of an anonymous AbReview

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

ab54210 at a 1 : 500 staining CDKN2A/p16INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB staining) is shown.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

ab54210 at a 1 : 500 staining CDKN2A/p16INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB) is shown.

• IHC-P

AbReview63857****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

Zinc buffered formalin-fixed human cartilage tissue stained for CDKN2A/p16INK4a using ab54210 at a 1/200 dilution for 15 hours at 4°C. A Goat-anti-mouse polyclonal-biotin was used at the secondary at a 1/200 dilution. For further detail please see abreview.

Image is courtesy of an anonymous abreview by a verified customer

• Flow Cyt

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Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab54210 (red line).

The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions.

Acquisition of >5,000 events was performed.

This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

ab54210 at a 1 : 500 staining CDKN2A/p16INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB staining) is shown.

• IHC-P

AbReview67297****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

10% formalin-fixed rat kidney tissue stained for CDKN2A/p16INK4a using ab54210 at a 1/500 dilution for 16 hours at 4°C. A Cy3-conjugated donkey-anti-mouse was used at the secondary at a 1/200 dilution. For further detail please see abreview.

Image is courtesy of an anonymous abreview by a verified customer

• IHC-P

AbReview17273****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210)

ab54210 staining CDKN2A/p16INK4a in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and blocked with 2% BSA for 1 hour at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% NGS/TBS) for 20 hours at 4°C. ab6785 (1/800) was used as the secondary antibody.

Image courtesy of an anonymous AbReview