Name: Anti-CDKN2A/p16INK4a antibody [2D9A12]
SKU: ab54210
Rating: 4 (19 reviews)

Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) is a mouse monoclonal antibody detecting CDKN2A/p16INK4a in Flow Cytometry, IHC-P. Suitable for Human, Rat.

- Over 140 publications

- Trusted since 2007

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (AB54210), expandable thumbnail

Publications

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: Preservative: 0.03% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human CDKN2A. The exact immunogen used to generate this antibody is proprietary information. Database link P42771

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt	WB
Human	Tested	Tested	Not recommended
Rat	Tested	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/200.00000 - 1/1000.00000	Notes Recommended antigen retrieval:Place slides in a special glass slide holder and fill in the rack with slides to ensure even heating.Rinse rack in 1L of Citrate buffer in ordinary pressure cooker,(0.01M,pH6.0) to immerse all slides.Cover the cap and heat for 15-20 minutes on electric cooker until water boils.Begin timing, only 2 minutes in high-pressureRemove the pressure cooker, Allow it to cool(Cool the slides at room temperature)Rinse the slides in PBS for 5 minutes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/200.00000 - 1/1000.00000	Notes Recommended antigen retrieval:Place slides in a special glass slide holder and fill in the rack with slides to ensure even heating.Rinse rack in 1L of Citrate buffer in ordinary pressure cooker,(0.01M,pH6.0) to immerse all slides.Cover the cap and heat for 15-20 minutes on electric cooker until water boils.Begin timing, only 2 minutes in high-pressureRemove the pressure cooker, Allow it to cool(Cool the slides at room temperature)Rinse the slides in PBS for 5 minutes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

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Target data

See full target information CDKN2A

Function

The protein expressed by the gene CDKN2A acts as a negative regulator of normal cell proliferation by strongly interacting with CDK4 and CDK6. This interaction inhibits the ability of CDK4 and CDK6 to interact with cyclins D and to phosphorylate the retinoblastoma protein. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CDKN2, MTS1, CDKN2A, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, CDK4I, MTS-1, p16-INK4, p16INK4A

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: Preservative: 0.03% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human CDKN2A. The exact immunogen used to generate this antibody is proprietary information. Database link P42771
Clone number: 2D9A12
Purification technique: Affinity purification Protein G
Concentration
Purification notes: Purified from tissue culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) is a mouse monoclonal antibody and is validated for use in Flow Cyt, IHC-P in human, rat samples.
Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) specifically detects CDKN2A/p16INK4a (UniProt ID: P42771; Molecular weight: 17kDa) and is sold in 100 µL selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) has high sensitivity and specificity.
Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) has been cited over 142 times in peer reviewed journals and is trusted by the scientific community.
Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) has 18 independent reviews from customers.

Target Information

CDKN2A, also known as cyclin-dependent kinase inhibitor 2A, is a crucial tumor suppressor gene located on chromosome 9p211. It encodes two proteins, p16INK4a and p14ARF, both of which play significant roles in regulating the cell cycle. p16INK4a inhibits cyclin-dependent kinases 4 and 6 (CDK4 and CDK6), preventing the progression from the G1 to S phase of the cell cycle. This inhibition is vital for controlling cell proliferation and preventing tumor growth. Mutations or deletions in CDKN2A are frequently associated with various cancers, including melanoma, pancreatic cancer, and head and neck squamous cell carcinoma.

Product promise

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8 product images

Image is courtesy of an anonymous customer review
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
ab54210 staining CDKN2A/p16INK4a in human cervix and ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000) for 20 minutes at 25°C. An undiluted HRP-conjugated mouse polymer was used as the secondary antibody.
Image is courtesy of an anonymous customer review by a verified customer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
Zinc buffered formalin-fixed human cartilage tissue stained for CDKN2A/p16INK4a using ab54210 at a 1/200 dilution for 15 hours at 4°C. A Goat-anti-mouse polyclonal-biotin was used at the secondary at a 1/200 dilution.
For further detail please see abreview.
Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab54210 (red line).
The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x10⁶ cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Image courtesy of an anonymous customer review
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
ab54210 staining CDKN2A/p16INK4a in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 2% BSA for 1 hour at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% NGS/TBS) for 20 hours at 4°C. Goat Anti-Mouse IgG H&L (FITC) ab6785 (1/800) was used as the secondary antibody.
Image is courtesy of an anonymous customer review by a verified customer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
10% formalin-fixed rat kidney tissue stained for CDKN2A/p16INK4a using ab54210 at a 1/500 dilution for 16 hours at 4°C. A Cy3-conjugated donkey-anti-mouse was used at the secondary at a 1/200 dilution.
For further detail please see abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
ab54210 at a 1:500 staining CDKN2A/p16INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB) is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
ab54210 at a 1:500 staining CDKN2A/p16INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB staining) is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
ab54210 at a 1:500 staining CDKN2A/p16INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB staining) is shown.