Anti-CDKN2A/p16INK4a antibody is a rabbit polyclonal antibody that is used to detect CDKN2A/p16INK4a in ICC/IF, western blot and IHC. Suitable for human and mouse samples.
- Cited in >70 publications
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- Nature communications 14:76192023Mid-old cells are a potential target for anti-aging interventions in the elderly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung Hwa Kim,Young-Kyoung Lee,Soon Sang Park,So Hyun Park,So Yeong Eom,Young-Sam Lee,Wonhee John Lee,Juhee Jang,Daeha Seo,Hee Young Kang,Jin Cheol Kim,Su Bin Lim,Gyesoon Yoon,Hong Seok Kim,Jang-Hee Kim,Tae Jun ParkPubMed 37993434
- Biomolecules 13:2023Early Resveratrol Treatment Mitigates Joint Degeneration and Dampens Pain in a Mouse Model of Pseudoachondroplasia (PSACH).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacqueline T Hecht,Alka C Veerisetty,Debabrata Patra,Mohammad G Hossain,Frankie Chiu,Claire Mobed,Francis H Gannon,Karen L PoseyPubMed 37892235
- Aging cell 22:e140002023Carnitine acetyltransferase deficiency mediates mitochondrial dysfunction-induced cellular senescence in dermal fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Ji Song,Chi-Hyun Park,Haesoo Kim,Sangbum Han,Si Hyung Lee,Dong Hun Lee,Jin Ho ChungPubMed 37828898
- Aging and disease 14:1778-17982023Clearance of Stress-Induced Premature Senescent Cells Alleviates the Formation of Abdominal Aortic Aneurysms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingfang Xie,Zhenquan Tang,Qiqi Chen,Xiaoqian Jia,Chuling Li,Ming Jin,Guoquan Wei,Hao Zheng,Xinzhong Li,Yanmei Chen,Wangjun Liao,Yulin Liao,Jianping Bin,Senlin HuangPubMed 37196124
- Theranostics 13:5207-52222023Upregulated FOXM1 stimulates chondrocyte senescence in Acot12Nudt7 double knockout mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinsoo Song,Ee Hyun Kim,Jun-Ho Yang,Donghyeon Kim,Akhmad Irhas Robby,Se-Ah Kim,Sung Young Park,Ji Hyun Ryu,Eun-Jung JinPubMed 37908734
- Aging 15:6658-66892023Inhibiting NLRP3 signaling in aging podocytes improves their life- and health-span.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalya Kaverina,R Allen Schweickart,Gek Cher Chan,Joseph C Maggiore,Diana G Eng,Yuting Zeng,Sierra R McKinzie,Hannah S Perry,Adilijiang Ali,Christopher O'Connor,Beatriz Maria Veloso Pereira,Ashleigh B Theberge,Joshua C Vaughan,Carol J Loretz,Anthony Chang,Neil A Hukriede,Markus Bitzer,Jeffrey W Pippin,Oliver Wessely,Stuart J ShanklandPubMed 37487005
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 71:301-3202023Developmental Expression of the Cell Cycle Regulator p16 in Retinal Glial Cells: A Novel Marker for Immature Ocular Astrocytes?Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Martinez-Fernandez de la Camara,Tina Storm,Ahmed Salman,Thomas Burgoyne,Martin Qvist Rasmussen,Harry O Orlans,Angela J Russell,Stephen G Davies,Alun R Barnard,Robert E MacLarenPubMed 37350564
- JCI insight 8:2023Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyao Li,Man J Livingston,Zhengwei Ma,Xiaoru Hu,Lu Wen,Han-Fei Ding,Daohong Zhou,Zheng DongPubMed 36917180
- Materials today. Bio 19:1006002023iPSC-sEVs alleviate microglia senescence to protect against ischemic stroke in aged mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Niu,Yuguo Xia,Lei Luo,Yu Chen,Ji Yuan,Juntao Zhang,Xianyou Zheng,Qing Li,Zhifeng Deng,Yang WangPubMed 36936398
- Hepatology international 17:377-3922022HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohan Cui,Xiao Yun,Meiling Sun,Renzhi Li,Xiajie Lyu,Yuanxiang Lao,Xihu Qin,Wenbin YuPubMed 36508088
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CDKN2A. The exact immunogen used to generate this antibody is proprietary information. Database link P42771
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Alternative names
CDKN2, MTS1, CDKN2A, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, CDK4I, MTS-1, p16-INK4, p16INK4A
Recommended products
Anti-CDKN2A/p16INK4a antibody is a rabbit polyclonal antibody that is used to detect CDKN2A/p16INK4a in ICC/IF, western blot and IHC. Suitable for human and mouse samples.
- Cited in >70 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CDKN2A. The exact immunogen used to generate this antibody is proprietary information. Database link P42771
- Purification technique
- Affinity purification Immunogen
- Specificity
Expression levels of the CDKN2A/p16INK4a protein may vary with sample type. It's barely expressed in normal tissue, and mostly expressed in some tumour tissues, such as cervical cancer, breast cancer and so on. Moreover, only expressed in some cell lines. Please see images for recommended positive controls.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse samples.
Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) specifically detects CDKN2A/p16INK4a (UniProt ID: P42771; Molecular weight: 17kDa) and is sold in 50 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) has high sensitivity and specificity.
Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) has been cited over 72 times in peer reviewed journals and is trusted by the scientific community.
Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) has 4 independent reviews from customers.
Target Information
CDKN2A, also known as cyclin-dependent kinase inhibitor 2A, is a crucial tumor suppressor gene located on chromosome 9p211. It encodes two proteins, p16INK4a and p14ARF, both of which play significant roles in regulating the cell cycle. p16INK4a inhibits cyclin-dependent kinases 4 and 6 (CDK4 and CDK6), preventing the progression from the G1 to S phase of the cell cycle. This inhibition is vital for controlling cell proliferation and preventing tumor growth. Mutations or deletions in CDKN2A are frequently associated with various cancers, including melanoma, pancreatic cancer, and head and neck squamous cell carcinoma.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CDKN2A/p16INK4a with ab189034 at 5 μg/ml. Heat mediated antigen retrieval was performed.
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)
Immunofuorescent analysis of human colon cells labeling CDKN2A using ab189034 at 20 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)
Immunohistochemical analysis of formalin fixed, paraffin embedded human colon tissue labeling CDKN2A using ab189034 at 5 μg/ml.
Western blot - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)
Western blot: Anti-CDKN2A antibody (ab189034) staining at 2 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab189034 was shown to bind specifically to CDKN2A however we recommend using Anti-CDKN2A/p16INK4a antibody [EPR24167-43] ab270058 for more specific binding in Western blot. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) at 2 µg/mL
Lane 1: HeLa cell lysate at 20 µg
Lane 2: HEK-293 cell lysate at 20 µg
Lane 3: Saos-2 cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 17 kDa
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