JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB252788

Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free

5

(2 Reviews)

|

(1 Publication)

Rat Recombinant Monoclonal p16INK4A antibody. Carrier free. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

P16ink4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (AB252788)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (AB252788)

Positive staining on paraffin-embedded mouse papilloma. The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The dilution of the recombinant antibody was 1 : 160 and the secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Counterstained with hematoxylin.

The image was kindly provided by Spanish National Cancer Research Centre (CNIO).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (AB252788)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (AB252788)

Image A : Positive staining on the wild-type mouse fibrosarcoma.

Image B : No staining on the p16 knock-out mouse fibrosarcoma.

The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The dilution of the recombinant antibody was 1 : 160 and the secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Paraffin-embedded sections. Counterstained with hematoxylin.

The image was kindly provided by Spanish National Cancer Research Centre (CNIO).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

PABLO33B

Isotype

IgG2a

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/160", "IHCP-species-notes": "<p></p>" } } }

Product details

ab252788 is the carrier-free version of ab241543.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
See full target information Cdkn2a

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Current protocols 4:e1030 PubMed38923763

2024

Applications of Surface Plasmon Resonance (SPR) to the Study of Diverse Protein-Ligand Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Dana M Burris,Samuel W Gillespie,Emma Joy Campbell,S Nick Ice,Vikas Yadav,William D Picking,Christian L Lorson,Kamal Singh
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com