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Rat Monoclonal p16INK4A antibody. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 23 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail

Publications

  • Kidney diseases (Basel, Switzerland) 8:408-4232022
    KYA1797K, a Novel Small Molecule Destabilizing β-Catenin, Is Superior to ICG-001 in Protecting against Kidney Aging.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Mingsheng Zhu,Xian Ling,Shan Zhou,Ping Meng,Qiyan Chen,Shuangqin Chen,Kunyu Shen,Chao Xie,Yaozhong Kong,Maosheng Wang,Lili Zhou
    PubMed 36466073
  • Frontiers in pharmacology 13:8364962022
    AMPK Activator O304 Protects Against Kidney Aging Through Promoting Energy Metabolism and Autophagy.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Mingsheng Zhu,Weiwei Shen,Jiemei Li,Nan Jia,Yabing Xiong,Jinhua Miao,Chao Xie,Qiyan Chen,Kunyu Shen,Ping Meng,Xiaolong Li,Qinyu Wu,Shan Zhou,Maosheng Wang,Yaozhong Kong,Lili Zhou
    PubMed 35308246

Key facts

Isotype

IgG2b

Host species

Rat

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FoFrICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Expected
Tested
Hamster
Not recommended
Not recommended
Not recommended
Not recommended

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Hamster, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Not recommended
Not recommended

Species

Hamster, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Hamster, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Hamster

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

    3 products for Alternative Product

      Target data

      Function

      Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

      Alternative names

      Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4, Cdkn2a, P16ink4a

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      Rat Monoclonal p16INK4A antibody. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 23 publications.

      Alternative names

      Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4, Cdkn2a, P16ink4a

      Key facts

      Isotype

      IgG2b

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      5-C3

      Purification technique

      Affinity purification Protein G

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.

      Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

      If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

      Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

        ab26696 staining CDKN2A/p19ARF in MEF1 cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab26696 at 5μg/ml and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2μg/ml (shown in red). The secondary antibody (shown in green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Alexa Fluor® 488 Goat anti-Rat IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

      • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

        Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).

      • Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

        Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

        Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.

        All lanes: Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

        Predicted band size: 18 kDa

      • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

        Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

        Blocking buffer: 2% BSA

        Gel type: MES

        All lanes: Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696) at 2 µg/mL

        All lanes: MEF-1 whole cell lysate at 20 µg

        Secondary

        All lanes: Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

        Predicted band size: 18 kDa

        Observed band size: 19 kDa, 85 kDa

        Exposure time: 1min

      • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

        Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

        All lanes: Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

        Lane 1: Total cell lysate from NIH3T3 cells with Arf gene deleted

        Lane 2: MEF total cell lysate

        Secondary

        All lanes: anti-rat IgG-HRP

        Predicted band size: 18 kDa

        Observed band size: 19 kDa

      Downloads

      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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