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Rat Monoclonal p16INK4A antibody. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 23 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail
  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Rat
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FoFrICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Expected
Tested
Hamster
Not recommended
Not recommended
Not recommended
Not recommended

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Hamster, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Not recommended
Not recommended

Species
Hamster, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Hamster, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Hamster
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

1 product for Alternative Product

Target data

Function

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

Alternative names

Recommended products

Rat Monoclonal p16INK4A antibody. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 23 publications.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
5-C3
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    ab26696 staining CDKN2A/p19ARF in MEF1 cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab26696 at 5μg/ml and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2μg/ml (shown in red). The secondary antibody (shown in green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Alexa Fluor® 488 Goat anti-Rat IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).

  • Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

    Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.

    All lanes: Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Predicted band size: 18 kDa

  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

    Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Blocking buffer: 2% BSA

    Gel type: MES

    All lanes: Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696) at 2 µg/mL

    All lanes: MEF-1 whole cell lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

    Predicted band size: 18 kDa

    Observed band size: 19 kDa, 85 kDa

    Exposure time: 1min

  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail

    Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    All lanes: Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Lane 1: Total cell lysate from NIH3T3 cells with Arf gene deleted

    Lane 2: MEF total cell lysate

    Secondary

    All lanes: anti-rat IgG-HRP

    Predicted band size: 18 kDa

    Observed band size: 19 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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