Rat Monoclonal p16INK4A antibody. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 23 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--immunocytochemistry-immunofluorescence-img169356.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)")
![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--immunocytochemistry-immunofluorescence-img71016.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)")
![Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--immunoprecipitation-img16136.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)")
![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--western-blot-img176883.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)")
![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--western-blot-img42024.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)")
Publications
Filter by
- Kidney diseases (Basel, Switzerland) 8:408-4232022KYA1797K, a Novel Small Molecule Destabilizing β-Catenin, Is Superior to ICG-001 in Protecting against Kidney Aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingsheng Zhu,Xian Ling,Shan Zhou,Ping Meng,Qiyan Chen,Shuangqin Chen,Kunyu Shen,Chao Xie,Yaozhong Kong,Maosheng Wang,Lili ZhouPubMed 36466073
- Frontiers in pharmacology 13:8364962022AMPK Activator O304 Protects Against Kidney Aging Through Promoting Energy Metabolism and Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingsheng Zhu,Weiwei Shen,Jiemei Li,Nan Jia,Yabing Xiong,Jinhua Miao,Chao Xie,Qiyan Chen,Kunyu Shen,Ping Meng,Xiaolong Li,Qinyu Wu,Shan Zhou,Maosheng Wang,Yaozhong Kong,Lili ZhouPubMed 35308246
- Frontiers in pharmacology 12:7854032021Pterostilbene Alleviates Cholestasis by Promoting SIRT1 Activity in Hepatocytes and Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanrui Ma,Jiaqing Xiang,Guixiao Huang,Yaxi Zhao,Xinyu Wang,Han Wu,Kewei Jiang,Zhen Liang,Lin Kang,Guangyan Yang,Shu YangPubMed 34899349
- Toxicology 441:1525022020Nicotine triggers islet β cell senescence to facilitate the progression of type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingli Sun et. al.PubMed 32473187
- Gene expression 20:39-522020Loss of ARF/INK4A Promotes Liver Progenitor Cell Transformation Toward Tumorigenicity Supporting Their Role in Hepatocarcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobyn P Strauss et. al.PubMed 32317048
- Nature cell biology 21:579-5912019ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Chu,Ning Kon,Delin Chen,Tongyuan Li,Tong Liu,Le Jiang,Shujuan Song,Omid Tavana,Wei GuPubMed 30962574
- Oncotarget 8:4373-43862016Ablating all three retinoblastoma family members in mouse lung leads to neuroendocrine tumor formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Lázaro,Miriam Pérez-Crespo,Ana Belén Enguita,Pilar Hernández,Jesús Martínez-Palacio,Marta Oteo,Julien Sage,Jesús M Paramio,Mirentxu SantosPubMed 27966456
- Oncogene 35:1988-952015Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC K Lee,Y Yang,C Chen,J LiuPubMed 26212014
- Cancer research 75:3167-802015TP53 Silencing Bypasses Growth Arrest of BRAFV600E-Induced Lung Tumor Cells in a Two-Switch Model of Lung Tumorigenesis.
- Nature immunology 16:458-662015The kinase Jnk2 promotes stress-induced mitophagy by targeting the small mitochondrial form of the tumor suppressor ARF for degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiao Zhang et. al.PubMed 25799126
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-FoFr | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Tested | Expected | Tested |
| Hamster | Not recommended | Not recommended | Not recommended | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster, Human | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
1 product for Alternative Product
Target data
Function
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Alternative names
P16ink4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4
Recommended products
Rat Monoclonal p16INK4A antibody. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 23 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 5-C3
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--immunocytochemistry-immunofluorescence-img169356.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)")
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
ab26696 staining CDKN2A/p19ARF in MEF1 cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab26696 at 5μg/ml and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2μg/ml (shown in red). The secondary antibody (shown in green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Alexa Fluor® 488 Goat anti-Rat IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--immunocytochemistry-immunofluorescence-img71016.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)")
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
![Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--immunoprecipitation-img16136.jpg "Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)")
Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.
All lanes: Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Predicted band size: 18 kDa
![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--western-blot-img176883.jpg "Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)")
Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Blocking buffer: 2% BSA
Gel type: MES
All lanes: Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696) at 2 µg/mL
All lanes: MEF-1 whole cell lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa, 85 kDa
Exposure time: 1min
![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696), expandable thumbnail](https://content.abcam.com/products/images/cdkn2a-p19arf-antibody-5-c3-ab26696--western-blot-img42024.jpg "Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)")
Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
All lanes: Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Lane 1: Total cell lysate from NIH3T3 cells with Arf gene deleted
Lane 2: MEF total cell lysate
SecondaryAll lanes: anti-rat IgG-HRP
Predicted band size: 18 kDa
Observed band size: 19 kDa
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