AB26696

Anti-CDKN2A/p19ARF antibody [5-C3]

Name: Anti-CDKN2A/p19ARF antibody [5-C3] Rat monoclonal (ab26696) | Abcam
Brand: Abcam Limited
SKU: AB26696
Price: 465 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(23 Publications)

Rat Monoclonal p16INK4A antibody. Suitable for IP, WB, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 23 publications.

View Alternative Names

P16ink4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

ab26696 staining CDKN2A/p19ARF in MEF1 cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab26696 at 5μg/ml and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor^® 647), at 2μg/ml (shown in red). The secondary antibody (shown in green) was ab150165, Alexa Fluor® 488 Goat anti-Rat IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).

Unknown

Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.

All lanes:

Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

Predicted band size: 18 kDa

false

Lab

Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Blocking buffer : 2% BSA

Gel type : MES

All lanes:

Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696) at 2 µg/mL

All lanes:

MEF-1 whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

Predicted band size: 18 kDa

Observed band size: 19 kDa,85 kDa

false

Exposure time: 1min

Unknown

Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

All lanes:

Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

Lane 1:

Total cell lysate from NIH3T3 cells with Arf gene deleted

Lane 2:

MEF total cell lysate

Secondary

All lanes:

anti-rat IgG-HRP

Predicted band size: 18 kDa

Observed band size: 19 kDa

false

HRP

HRP Anti-CDKN2A/p19ARF antibody [5-C3]
Carrier free

Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

5-C3

Isotype

IgG2b

Carrier free

Reacts with

Mouse

Applications

WB, IHC-FoFr, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Hamster": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

See full target information Cdkn2a

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Kidney diseases (Basel, Switzerland) 8:408-423 PubMed36466073

2022

KYA1797K, a Novel Small Molecule Destabilizing β-Catenin, Is Superior to ICG-001 in Protecting against Kidney Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Mingsheng Zhu,Xian Ling,Shan Zhou,Ping Meng,Qiyan Chen,Shuangqin Chen,Kunyu Shen,Chao Xie,Yaozhong Kong,Maosheng Wang,Lili Zhou

Frontiers in pharmacology 13:836496 PubMed35308246

2022

AMPK Activator O304 Protects Against Kidney Aging Through Promoting Energy Metabolism and Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Mingsheng Zhu,Weiwei Shen,Jiemei Li,Nan Jia,Yabing Xiong,Jinhua Miao,Chao Xie,Qiyan Chen,Kunyu Shen,Ping Meng,Xiaolong Li,Qinyu Wu,Shan Zhou,Maosheng Wang,Yaozhong Kong,Lili Zhou

Frontiers in pharmacology 12:785403 PubMed34899349

2021

Pterostilbene Alleviates Cholestasis by Promoting SIRT1 Activity in Hepatocytes and Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanrui Ma,Jiaqing Xiang,Guixiao Huang,Yaxi Zhao,Xinyu Wang,Han Wu,Kewei Jiang,Zhen Liang,Lin Kang,Guangyan Yang,Shu Yang

Toxicology 441:152502 PubMed32473187

2020

Nicotine triggers islet β cell senescence to facilitate the progression of type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Lingli Sun,Xiaohua Wang,Tianye Gu,Baoying Hu,Jiashan Luo,Yi Qin,Chunhua Wan

Gene expression 20:39-52 PubMed32317048

2020

Loss of ARF/INK4A Promotes Liver Progenitor Cell Transformation Toward Tumorigenicity Supporting Their Role in Hepatocarcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Robyn P Strauss,Katherine M Audsley,Adam M Passman,Joanne H van Vuuren,Megan L Finch-Edmondson,Bernard A Callus,George C Yeoh

Nature cell biology 21:579-591 PubMed30962574

2019

ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chu,Ning Kon,Delin Chen,Tongyuan Li,Tong Liu,Le Jiang,Shujuan Song,Omid Tavana,Wei Gu

Oncotarget 8:4373-4386 PubMed27966456

2016

Ablating all three retinoblastoma family members in mouse lung leads to neuroendocrine tumor formation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Lázaro,Miriam Pérez-Crespo,Ana Belén Enguita,Pilar Hernández,Jesús Martínez-Palacio,Marta Oteo,Julien Sage,Jesús M Paramio,Mirentxu Santos

Oncogene 35:1988-95 PubMed26212014

2015

Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death.

Applications

Unspecified application

Species

Unspecified reactive species

C K Lee,Y Yang,C Chen,J Liu

Cancer research 75:3167-80 PubMed26001956

2015

TP53 Silencing Bypasses Growth Arrest of BRAFV600E-Induced Lung Tumor Cells in a Two-Switch Model of Lung Tumorigenesis.

Applications

Species

Unspecified reactive species

Anny Shai,David Dankort,Joseph Juan,Shon Green,Martin McMahon

Nature immunology 16:458-66 PubMed25799126

2015

The kinase Jnk2 promotes stress-induced mitophagy by targeting the small mitochondrial form of the tumor suppressor ARF for degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiao Zhang,Hong Kuang,Cong Chen,Jie Yan,Hanh Chi Do-Umehara,Xin-yuan Liu,Laura Dada,Karen M Ridge,Navdeep S Chandel,Jing Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDKN2A/p19ARF antibody [5-C3]

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (AB26696)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (23)

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