Anti-CDKN2A/p19ARF antibody (ab80) is a rabbit polyclonal antibody detecting CDKN2A/p19ARF in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Mouse.
- Over 220 publications
- Trusted since 2002
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Publications
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- Aging and disease 14:2215-22372023IFNγ Transcribed by IRF1 in CD4+ Effector Memory T Cells Promotes Senescence-Associated Pulmonary Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyun Chen,Qiuyi Wang,Jie Li,Yuan Li,Ao Chen,Jiawen Zhou,Jingyu Zhao,Zhiyuan Mao,Zihao Zhou,Jin'ge Zhang,Yue Wang,Rong Wang,Qing Li,Yongjie Zhang,Runqiu Jiang,Dengshun Miao,Jianliang JinPubMed 37199578
- Metabolites 12:2022-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNguyen Khanh Hoang,Eiji Maegawa,Shigeru Murakami,Stephen W Schaffer,Takashi ItoPubMed 35448536
- The Journal of clinical investigation 132:2022Age-related GSK3β overexpression drives podocyte senescence and glomerular aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYudong Fang,Bohan Chen,Zhangsuo Liu,Athena Y Gong,William T Gunning,Yan Ge,Deepak Malhotra,Amira F Gohara,Lance D Dworkin,Rujun GongPubMed 35166234
- JID innovations : skin science from molecules to population health 2:1000762022A Series of BRAF- and NRAS-Driven Murine Melanoma Cell Lines with Inducible Gene Modulation Capabilities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlah Bok,Ariana Angarita,Stephen M Douglass,Ashani T Weeraratna,Florian A KarrethPubMed 35146482
- Aging 14:195-2242022Endothelial and systemic upregulation of miR-34a-5p fine-tunes senescence in progeria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristina Manakanatas,Santhosh Kumar Ghadge,Azra Agic,Fatih Sarigol,Petra Fichtinger,Irmgard Fischer,Roland Foisner,Selma Osmanagic-MyersPubMed 35020601
- Laboratory investigation; a journal of technical methods and pathology 102:391-4002021A murine mesenchymal stem cell model for initiating events in osteosarcomagenesis points to CDK4/CDK6 inhibition as a therapeutic target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatasja Franceschini,Raffaele Gaeta,Paul Krimpenfort,Inge Briaire-de Bruijn,Alwine B Kruisselbrink,Karoly Szuhai,Ieva Palubeckaitė,Anne-Marie Cleton-Jansen,Judith V M G BovéePubMed 34921235
- PLoS genetics 17:e10099482021Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPooja Flora,Meng-Yen Li,Phillip M Galbo,Maider Astorkia,Deyou Zheng,Elena EzhkovaPubMed 34905545
- Science advances 7:2021Differential H4K16ac levels ensure a balance between quiescence and activation in hematopoietic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCecilia Pessoa Rodrigues,Asifa AkhtarPubMed 34362741
- NPJ precision oncology 5:142021Stabilization of p18 by deubiquitylase CYLD is pivotal for cell cycle progression and viral replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYueshuo Li,Feng Shi,Jianmin Hu,Longlong Xie,Lin Zhao,Min Tang,Xiangjian Luo,Mao Ye,Hui Zheng,Min Zhou,Na Liu,Ann M Bode,Jia Fan,Jian Zhou,Qiang Gao,Shuangjian Qiu,Weizhong Wu,Xin Zhang,Weihua Liao,Ya CaoPubMed 33654169
- Cancer research 81:2442-24562021The Common Germline Mutation Is Hypomorphic and Confers Incomplete Penetrance and Late Tumor Onset in a Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn R Jeffers,Emilia M Pinto,Jerold E Rehg,Michael R Clay,Jinling Wang,Geoffrey Neale,Richard J Heath,Guillermina Lozano,Enzo Lalli,Bonald C Figueiredo,Alberto S Pappo,Carlos Rodriguez-Galindo,Wenan Chen,Stanley Pounds,Raul C Ribeiro,Gerard P ZambettiPubMed 33637564
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC | ELISA | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Expected | Tested | Expected | Expected | Expected |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle). |
Species Human | Dilution info - | Notes Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle). |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples. |
Species Human | Dilution info - | Notes Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples. |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Capable of inducing cell cycle arrest in G1 and G2 phases (PubMed:8521522, PubMed:9393858). Acts as a tumor suppressor (PubMed:15601844, PubMed:17936562, PubMed:8521522, PubMed:9393858). Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus (PubMed:10359817, PubMed:9529248). This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis (PubMed:10359817). Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes (PubMed:15361884). Binds to BCL6 and down-regulates BCL6-induced transcriptional repression (PubMed:15567177). Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression (By similarity). Binds to TOP1/TOPOI and stimulates its activity (By similarity). This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation (By similarity). Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing (By similarity). Plays a role in inhibiting ribosome biogenesis, perhaps by binding to the nucleolar localization sequence of transcription termination factor TTF1, and thereby preventing nucleolar localization of TTF1 (PubMed:20513429). Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination (By similarity). Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1 (By similarity). Binds to HUWE1 and represses its ubiquitin ligase activity (By similarity). May play a role in controlling cell proliferation and apoptosis during mammary gland development (By similarity). Isoform smARF. May be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.
Alternative names
Tumor suppressor ARF, Alternative reading frame, Cyclin-dependent kinase inhibitor 2A, p19ARF, ARF, Cdkn2a
Recommended products
Anti-CDKN2A/p19ARF antibody (ab80) is a rabbit polyclonal antibody detecting CDKN2A/p19ARF in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Mouse.
- Over 220 publications
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
On Western blot the antibody reveals IVT murine p19ARF and a band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs.
Replenishment batches of ab80 are tested in WB. Previous batches were additionally validated in ELISA, ICC, ICC/IF, IHC-Fr and IHC-P. These applications are still expected to work and are covered by our Abcam product promise.
- Concentration
- Purification notes
The serum has been purified on a peptide-NHS-activated Sepharose column. Specific antibodies have been eluted with 100 mM glycine pH 2.5. The purified antibodies have been dialysed against PBS pH 7.4.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CDKN2A/p19ARF antibody (ab80) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Mouse samples.
What is the molecular weight of CDKN2A/p19ARF?
Anti-CDKN2A/p19ARF (ab80) specifically detects a band for CDKN2A/p19ARF (UniProt: Q64364) at a molecular weight of 17kDa.
Trusted by the scientific community
Anti-CDKN2A/p19ARF (ab80) was first used in a scientific publication in 2002 and has been cited over 220 times in peer-reviewed journals.
Reviewed by scientists
Anti-CDKN2A/p19ARF (ab80) has over 15 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
This image is courtesy of Charles SherrWestern blot - Anti-CDKN2A/p19ARF antibody (ab80)
Western blot of primary Mouse Embryo Fibroplasts (MEFs) using ab80 at 1 μg/ml. (30 μg of protein from total cell extracts per lane.)
All lanes: Western blot - Anti-CDKN2A/p19ARF antibody (ab80) at 1 µg/mL
Lanes 1 - 2: mouse embryonic fibroblast total cell extract. at 30 µg
Lane 3: mouse embryonic fibroblast total cell extract, with the p19ARF gene knocked out as a negative control. at 30 µg
Performed under reducing conditions.
Predicted band size: 18 kDa
Pictures were generously provided by Dr. David BertwistleImmunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80)
ab80 antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+).
Cells grown on coverslips were fixed with a 1:1 mixture of methanol:acetone at -20C for 10 mins. After drying, rehydrating and blocking, the co
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