Rabbit Polyclonal CDKN2AIP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CDKN2AIP aa 1-50.
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Publications
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- Molecular cancer 21:922022Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Wang,Anqi Sun,Aihui Yan,Jian Yao,Haibo Huang,Ziming Gao,Tao Han,Jia Gu,Ni Li,Huizhe Wu,Kai LiPubMed 35366893
- Cell discovery 6:852020CARF promotes spermatogonial self-renewal and proliferation through Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhao Cui et. al.PubMed 33298864
- Carcinogenesis 35:138-442013Synergistic anti-breast cancer effect of a combined treatment with the methyl donor S-adenosyl methionine and the DNA methylation inhibitor 5-aza-2'-deoxycytidine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlora Chik,Ziv Machnes,Moshe SzyfPubMed 23985780
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CDKN2AIP aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NXV6
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Rabbit | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Rabbit | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Regulates DNA damage response in a dose-dependent manner through a number of signaling pathways involved in cell proliferation, apoptosis and senescence.
Alternative names
CARF, CDKN2AIP, CDKN2A-interacting protein, Collaborator of ARF
Recommended products
Rabbit Polyclonal CDKN2AIP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CDKN2AIP aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CDKN2AIP aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NXV6
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab140519 was affinity purified using an epitope specific to CDKN2AIP immobilized on a solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CDKN2AIP also known as CARF (CDKN2A interacting protein) is a protein involved in cell cycle regulation. It plays a mechanical role by stabilizing the tumor suppressor protein p53 influencing cell proliferation and senescence. CARF is a 12 kDa protein expressed in various tissues with notable levels in the brain and certain tumor tissues. The protein interacts with the CDKN2A locus which encodes the similar tumor suppressor proteins p16 and p14ARF leading to its alternate name association.
- Biological function summary
The protein CDKN2AIP/CARF participates in modulating cellular response to DNA damage. It forms complexes with p53 and other regulators like HDM2 to control cell cycle checkpoints and apoptosis. This regulatory mechanism ensures cells with damaged DNA do not progress through the cell cycle reducing the risk of malignant transformations. Additionally CDKN2AIP/CARF influences the expression of genes associated with cellular aging and stress response impacting how a cell responds to various biological signals.
- Pathways
CDKN2AIP/CARF influences the p53 signaling pathway a critical axis in maintaining genomic integrity. It partners with p53 essentially regulating cell cycle arrest senescence and apoptosis which is a protective measure against cancer development. CARF interacts closely with CDKN2A and other elements in the retinoblastoma protein (pRb) pathway highlighting its role in regulating cell division and ensuring proper cell cycle progression.
- Associated diseases and disorders
CDKN2AIP/CARF links to cancers such as glioblastoma and melanoma due to its influence on the p53 pathway. Its interaction with p53 and p14ARF suggests a role in tumor suppression mechanisms that when disrupted could lead to uncontrolled cell proliferation. Additionally dysregulation of CDKN2AIP/CARF expression may also relate to age-related disorders as its influence on cellular senescence impacts processes like aging and neurodegeneration. Because of its associations with p53 in these pathways it poses as a potential therapeutic target in cases where these mechanisms fail highlighting its relevance in disease intervention strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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2 product images
Western blot - Anti-CDKN2AIP antibody (ab140519)
All lanes: Western blot - Anti-CDKN2AIP antibody (ab140519) at 0.4 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30s
Immunoprecipitation - Anti-CDKN2AIP antibody (ab140519)
Immunoprecipitation of CDKN2AIP from 293T whole cell lysate using ab140519 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
For blotting immunoprecipitated CDKN2AIP, ab140519 was used at 1 µg/ml.
Detection: chemiluminescence with an exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-CDKN2AIP antibody (ab140519)
Predicted band size: 61 kDa
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