AB140519

Anti-CDKN2AIP antibody

Name: Anti-CDKN2AIP antibody (ab140519) | Abcam
Brand: Abcam Limited
SKU: AB140519
Price: 520 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(3 Publications)

Rabbit Polyclonal CDKN2AIP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CDKN2AIP aa 1-50.

View Alternative Names

CARF, CDKN2AIP, CDKN2A-interacting protein, Collaborator of ARF

2 Images

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Immunoprecipitation - Anti-CDKN2AIP antibody (AB140519)

Immunoprecipitation of CDKN2AIP from 293T whole cell lysate using ab140519 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
For blotting immunoprecipitated CDKN2AIP, ab140519 was used at 1 µg/ml.
Detection : chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CDKN2AIP antibody (ab140519)

Predicted band size: 61 kDa

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Western blot - Anti-CDKN2AIP antibody (AB140519)

All lanes:

Western blot - Anti-CDKN2AIP antibody (ab140519) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 61 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CDKN2AIP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NXV6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab140519 was affinity purified using an epitope specific to CDKN2AIP immobilized on a solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDKN2AIP also known as CARF (CDKN2A interacting protein) is a protein involved in cell cycle regulation. It plays a mechanical role by stabilizing the tumor suppressor protein p53 influencing cell proliferation and senescence. CARF is a 12 kDa protein expressed in various tissues with notable levels in the brain and certain tumor tissues. The protein interacts with the CDKN2A locus which encodes the similar tumor suppressor proteins p16 and p14ARF leading to its alternate name association.

Biological function summary

The protein CDKN2AIP/CARF participates in modulating cellular response to DNA damage. It forms complexes with p53 and other regulators like HDM2 to control cell cycle checkpoints and apoptosis. This regulatory mechanism ensures cells with damaged DNA do not progress through the cell cycle reducing the risk of malignant transformations. Additionally CDKN2AIP/CARF influences the expression of genes associated with cellular aging and stress response impacting how a cell responds to various biological signals.

Pathways

CDKN2AIP/CARF influences the p53 signaling pathway a critical axis in maintaining genomic integrity. It partners with p53 essentially regulating cell cycle arrest senescence and apoptosis which is a protective measure against cancer development. CARF interacts closely with CDKN2A and other elements in the retinoblastoma protein (pRb) pathway highlighting its role in regulating cell division and ensuring proper cell cycle progression.

CDKN2AIP/CARF links to cancers such as glioblastoma and melanoma due to its influence on the p53 pathway. Its interaction with p53 and p14ARF suggests a role in tumor suppression mechanisms that when disrupted could lead to uncontrolled cell proliferation. Additionally dysregulation of CDKN2AIP/CARF expression may also relate to age-related disorders as its influence on cellular senescence impacts processes like aging and neurodegeneration. Because of its associations with p53 in these pathways it poses as a potential therapeutic target in cases where these mechanisms fail highlighting its relevance in disease intervention strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates DNA damage response in a dose-dependent manner through a number of signaling pathways involved in cell proliferation, apoptosis and senescence.

See full target information CDKN2AIP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 21:92 PubMed35366893

2022

Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Anqi Sun,Aihui Yan,Jian Yao,Haibo Huang,Ziming Gao,Tao Han,Jia Gu,Ni Li,Huizhe Wu,Kai Li

Cell discovery 6:85 PubMed33298864

2020

CARF promotes spermatogonial self-renewal and proliferation through Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhao Cui,Xiaoli He,Xiaohong Zhai,Huan Zhang,Yuanwei Zhang,Fei Jin,Xiaomin Song,Dianqing Wu,Qinghua Shi,Lin Li

Carcinogenesis 35:138-44 PubMed23985780

2013

Synergistic anti-breast cancer effect of a combined treatment with the methyl donor S-adenosyl methionine and the DNA methylation inhibitor 5-aza-2'-deoxycytidine.

Applications

Unspecified application

Species

Unspecified reactive species

Flora Chik,Ziv Machnes,Moshe Szyf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CDKN2AIP antibody

Immunoprecipitation - Anti-CDKN2AIP antibody (AB140519)

Western blot - Anti-CDKN2AIP antibody (AB140519)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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