AB175393

Anti-CDKN3 antibody

Name: Anti-CDKN3 antibody (ab175393) | Abcam
Brand: Abcam Limited
SKU: AB175393
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal CDKN3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDKN3.

View Alternative Names

CDI1, CIP2, KAP, CDKN3, Cyclin-dependent kinase inhibitor 3, CDK2-associated dual-specificity phosphatase, Cyclin-dependent kinase interactor 1, Cyclin-dependent kinase-interacting protein 2, Kinase-associated phosphatase

2 Images

Supplier Data

Western blot - Anti-CDKN3 antibody (AB175393)

All lanes:

Western blot - Anti-CDKN3 antibody (ab175393) at 1/500 dilution

All lanes:

Mouse heart extract

Predicted band size: 24 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-CDKN3 antibody (AB175393)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDKN3 antibody (AB175393)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175393. Blue DAPI for nuclear staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDKN3.

Q16667

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein CDKN3 also known as Cyclin-Dependent Kinase Inhibitor 3 functions primarily as a phosphatase. It removes phosphate groups from molecules specifically targeting the phosphorylated threonine and serine residues. This action regulates cell cycle progression enabling proper cell division. CDKN3 has a molecular mass of approximately 42 kDa. This protein expresses widely in human tissues with significant levels found in the liver lung and heart. Its regulation and expression are important for maintaining normal cellular functions.

Biological function summary

CDKN3 plays a fundamental role in cell cycle control. It forms complexes with various cyclin-dependent kinases (CDKs) particularly CDK1 and CDK2 inhibiting their activity. By controlling these CDKs CDKN3 ensures proper transition through cell cycle phases. This regulation prevents unplanned cell proliferation and maintains genomic integrity. CDKN3's interaction with CDKs highlights its importance in controlling cell growth and preventing the cell cycle's derailment.

Pathways

CDKN3's role significantly impacts the cell cycle and DNA damage response pathways. It influences the G1/S and G2/M checkpoints through its interaction with CDK1 and CDK2 forming an integral part of these critical pathways that control cell division timing. CDKN3 also extends its influence to other proteins like Cyclin A and Cyclin B impacting their function and stability during cell cycle regulation. These interactions underpin CDKN3's involvement in ensuring that cells only proceed to divide when conditions are optimal.

CDKN3 has been implicated in the development of various cancers including liver and bladder cancer. Alterations in CDKN3 expression or function can lead to unchecked cell division and tumor progression. In the context of these cancers CDKN3 often interacts with other proteins like CDC25A which further influences its role in cell proliferation. By understanding these disease-related interactions researchers can better target CDKN3 for therapeutic interventions aimed at restoring normal cell cycle control in cancerous tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell cycle regulation. Dual specificity CC phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues (PubMed : 8127873, PubMed : 8242750). Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner (PubMed : 7569954).

See full target information CDKN3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 23:101-117 PubMed36406252

2022

Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaijie Li,Liyan Zhang,Chunyu Liu,Jua Kim,Kun Su,Tingli Chen,Limin Zhao,Xiaomei Lu,Hao Zhang,Yinglin Cui,Xu Cui,Feng Yuan,Haobo Pan

Developmental cell 57:1712-1727.e9 PubMed35809563

2022

A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.

Applications

Unspecified application

Species

Unspecified reactive species

Dilyana Georgieva Kirova,Kristyna Judasova,Julia Vorhauser,Thomas Zerjatke,Jacky Kieran Leung,Ingmar Glauche,Jörg Mansfeld

Biochemical pharmacology 178:114048 PubMed32446889

2020

Novel epoxy-tiglianes stimulate skin keratinocyte wound healing responses and re-epithelialization via protein kinase C activation.

Applications

Unspecified application

Species

Unspecified reactive species

Rachael L Moses,Glen M Boyle,Rachel A Howard-Jones,Rachel J Errington,Jenny P Johns,Victoria Gordon,Paul Reddell,Robert Steadman,Ryan Moseley

Oncology letters 19:542-548 PubMed31897169

2020

CDKN3 promotes cell proliferation, invasion and migration by activating the AKT signaling pathway in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxu Yu,Jun Yao,Mingyu Du,Jinjun Ye,Xia He,Li Yin

American journal of physiology. Lung cellular and molecular physiology 316:L918-L933 PubMed30628487

2019

Tumor-suppressive effects of microRNA-181d-5p on non-small-cell lung cancer through the CDKN3-mediated Akt signaling pathway in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ming Gao,Yue Zheng,Ping Wang,Lei Zheng,Wen-Li Zhang,Ya Di,Lan-Lan Chen,Xiao-Bo Yin,Qi Tian,Shan-Shan Shi,Shu-Feng Xu

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDKN3 antibody

Western blot - Anti-CDKN3 antibody (AB175393)

Immunocytochemistry/ Immunofluorescence - Anti-CDKN3 antibody (AB175393)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise