JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB205551

Anti-CDNF antibody [6G5]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CDNF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDNF.

View Alternative Names

ARMETL1, CDNF, Cerebral dopamine neurotrophic factor, ARMET-like protein 1, Conserved dopamine neurotrophic factor

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CDNF antibody [6G5] (AB205551)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDNF antibody [6G5] (AB205551)

Immunofluorescent analysis of U2OS cells labeling CDNF with ab205551 at 1 μg/ml. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody. Counterstained with DAPI (blue).

Left panel : CDNF expressing U2OS cells.
Right panel : Negative control (non-transfected U2OS cells).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [6G5] (AB205551)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [6G5] (AB205551)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer's disease patients labeling CDNF with ab205551 at 5 μg/ml. Image on the right shows negative staining without primary antibody. Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium.

Western blot - Anti-CDNF antibody [6G5] (AB205551)
  • WB

Supplier Data

Western blot - Anti-CDNF antibody [6G5] (AB205551)

All lanes:

Western blot - Anti-CDNF antibody [6G5] (ab205551) at 0.5 µg/mL

All lanes:

CDNF protein expressed into the supernatant of CHO cell culture medium

Predicted band size: 21 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6G5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDNF.

Q49AH0

style
left-column

Complementary Products

Assay Kits

AB260071

Human CDNF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CDNF ELISA Kit (AB260071)

  • 0
  • 0
Assay Kits

AB260071

Human CDNF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CDNF ELISA Kit (AB260071)

  • 0
  • 0
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB254257

Anti-POMC antibody [EPR22534-165]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] (AB254257)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/300", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/300", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDNF also known as Cerebral Dopamine Neurotrophic Factor is a protein that helps in maintaining the proper function of neurons. It has a mass of approximately 18 kDa. This protein is widely expressed in various tissues especially in the brain. CDNF maintains endoplasmic reticulum homeostasis and is involved in the stabilization of dopaminergic neurons. It does this by reducing stress in the endoplasmic reticulum and promoting cell survival mechanisms.
Biological function summary

The protein ensures the survival of neurons by modulating cellular stress pathways. CDNF is not known to be part of a larger protein complex. Instead its activity relies on its own structural configuration to influence stress responses and neuron protection. As a secreted protein it directly interacts with and influences cellular mechanisms within the extracellular space contributing to a healthy neuronal environment.

Pathways

CDNF interacts with at least oxidative stress response pathways and neuroprotection pathways. These pathways underpin its role in counteracting cellular stress related to neurodegeneration. For example CDNF shares a functional connection with proteins like MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) another neurotrophic factor involved in similar neuroprotective pathways enhancing neuronal survival and recovery from cellular stress.

Neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease are major areas where CDNF shows relevance. In Parkinson's disease CDNF's neuroprotective role helps mitigate the loss of dopaminergic neurons. There is evidence linking CDNF with MANF which also exhibits protective roles in neuronal health. Both proteins aim to promote neuron survival amid progression of neurodegeneration associated with these diseases indicating their potential as therapeutic agents.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).
See full target information CDNF
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Infection and immunity 87: PubMed31182619

2019

Persistent Infection of the Brain Induced Neurodegeneration Associated with Activation of Complement and Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,Emily G Severance,Raphael P Viscidi,Robert H Yolken,Jianchun Xiao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com