Anti-CDNF antibody [EPR22334-99]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] (AB253238)

Immunohistochemistry analysis of Human skeletal muscle using ab253238 at 1/100 dilution. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. Positive staining on human skeletal muscle. The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Unknown

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (AB253238)

CDNF was immunoprecipitated from 0.35 mg human testis tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

Lane 1 : Human testis tissue lysate 10 μg (input)
Lane 2 : ab253238 IP in human testis tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253238 in human testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)

Predicted band size: 21 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (AB253238)

CDNF was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

Lane 1 : Human skeletal muscle tissue lysate 10 μg (input)
Lane 2 : ab253238 IP in human skeletal muscle tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253238 in human skeletal muscle lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)

Predicted band size: 21 kDa

false

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] (AB253238)

Immunohistochemistry analysis of (A) Heart tissue from wild-type C57BL/6JGpt mice (B) Heart tissue from CDNF knockout mice using ab253238 at 1/100. Positive staining on (A) Heart tissue from wild-type C57BL/6JGpt mice and no staining on (B) Heart tissue from CDNF knockout mice. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CDNF-KO homozygous mice (Strain ID : T031749). The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] (AB253238)

Immunohistochemistry analysis of rat heart using ab253238 at 1/100 dilution. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. Positive staining on rat heart. The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] (AB253238)

Immunohistochemistry analysis of (A) Testis tissue from wild-type C57BL/6JGpt mice (B) Testis tissue from CDNF knockout mice using ab253238 at 1/100. Positive staining on (A) Heart tissue from wild-type C57BL/6JGpt mice and no staining on (B) Heart tissue from CDNF knockout mice. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. Positive staining on (A) Testis tissue from wild-type C57BL/6JGpt mice and no staining on (B) Testis tissue from CDNF knockout mice. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CDNF-KO homozygous mice (Strain ID : T031749). The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumenteval Solution2) for 20 mins

• WB

Unknown

Western blot - Anti-CDNF antibody [EPR22334-99] (AB253238)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Kidney and brain are CDNF low expressing tissues (PMID : 17611540).

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 21 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Anti-CDNF antibody [EPR22334-99] (AB253238)

Exposure times : Lanes 1 and 2 : 37 seconds; Lanes 3 and 4 : 3 minutes.

Blocking/diluting buffer and concentration : 5% NFDM/TBST

The expression profile and molecular weight observed are consistent with literature (PMID : 17611540).

All lanes:

Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle lysate at 20 µg

Lane 3:

Human skeletal muscle lysate at 20 µg

Lane 4:

Human testis lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 4:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 17 kDa

false

• WB

Lab

Western blot - Anti-CDNF antibody [EPR22334-99] (AB253238)

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CDNF-KO homozygous mice (Strain ID : T031749).

All lanes:

Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

Lane 1:

CDNF knockout mouse brain tissue lysate

Lane 2:

Wild-type mouse brain tissue lysate

Lane 3:

CDNF knockout mouse gastrocnemius muscle tissue lysate

Lane 4:

Wild-type mouse gastrocnemius muscle tissue lysate

Lane 5:

CDNF knockout mouse heart tissue lysate

Lane 6:

Wild-type mouse heart tissue lysate

Lane 7:

CDNF knockout mouse soleus muscle tissue lysate

Lane 8:

Wild-type mouse soleus muscle tissue lysate

Lane 9:

CDNF knockout mouse testis tissue lysate

Lane 10:

Wild-type mouse testis tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 26s

• WB

Unknown

Western blot - Anti-CDNF antibody [EPR22334-99] (AB253238)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

All lanes:

Recombinant Human CDNF Protein (aa25-187) 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 17 kDa

false

Exposure time: 3min